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Study on the Growth and Survival Rate of Capsulated Microalgae and Macroalgae Zoospores

미세조류 및 해조류 유주자의 피막화를 통한 생장 및 생존율 연구

  • Jung, Sang-Mok (Dept. of Life Science and Biotechnology, Soonchunhyang Univ.) ;
  • Lee, Han-Seong (Korea Coast Guard) ;
  • Kang, Seul-Gi (Dept. of Life Science and Biotechnology, Soonchunhyang Univ.) ;
  • Son, Ji-Su (Dept. of Life Science and Biotechnology, Soonchunhyang Univ.) ;
  • Jeon, Jae-Hyuk (Dept. of Life Science and Biotechnology, Soonchunhyang Univ.) ;
  • Lee, Han-Joo (Dept. of Life Science and Biotechnology, Soonchunhyang Univ.) ;
  • Shin, Hyun-Woung (Dept. of Life Science and Biotechnology, Soonchunhyang Univ.)
  • 정상목 (순천향대학교 생명시스템학과) ;
  • 이한성 (해양경비안전본부) ;
  • 강슬기 (순천향대학교 생명시스템학과) ;
  • 손지수 (순천향대학교 생명시스템학과) ;
  • 전재혁 (순천향대학교 생명시스템학과) ;
  • 이한주 (순천향대학교 생명시스템학과) ;
  • 신현웅 (순천향대학교 생명시스템학과)
  • Received : 2016.01.14
  • Accepted : 2016.02.17
  • Published : 2016.02.29

Abstract

The purpose of this study was to improve growth and survival rate of marine microalgae and macroalga zoospores using with eco-friendly capsulation materials. The capsulation materials were chosen an alginic acid which extracted from marine brown algae combining with starch and calcium chloride. The capsulated microalgae, Nannochloropsis salina and macroalga zoospores, Ulva australis were evaluated with growth and survival rate. When the mixed ratio of alginic acid was less than 50%, capsule formation was not performed. When the ratio of 50% alginic acid and 50% starch, the microalgae was shown the highest growth and survival rate increasing up to $8.74{\times}10^5cells\;mL^{-1}$ while 100% of alginic acid was the lowest rate up to $4.92{\times}10^5cells\;mL^{-1}$. The increasing starch ratio improved to their growth and survival rate, however decreasing alginic acid make physical capsule formation weaken. By applying on a surface of artificial reef, capsulated algal zoospores were germinated 99 individuals $cm^{-2}$. This attempt will be provided to basic core technology for marine afforestation in coastal area.

본 연구는 미세조류 및 해조류 유주자의 피막화가 이들의 생장 및 생존에 미치는 영향을 파악할 목적으로 수행되었다. 갈조류의 세포벽을 구성하는 녹말과 알긴산을 다양한 비율로 혼합하여 친환경 피막소재로 이용하였으며, 염화칼슘수용액에 적하(form a drop)한 후 피막의 형성 여부를 확인하였고, 미세조류 Nannochloropsis salina와 해조류 Ulva australis의 유주자를 피막화하여 생존율 및 성장률을 측정하였다. 미세조류와 해조류 피막은 녹말과 알긴산의 배합비(무게비)가 5:5, 2:8, 0:10일 때에만 형성되었으며, 10:0과 8:2에서는 형성되지 않았다. 형성된 피막 중 녹말과 알긴산의 5:5 배합비에서 미세조류 개체수가 $8.74{\times}10^5cells\;mL^{-1}$로 최대 증가를 보였으나, 알긴산만을 이용한 피막에서는 $4.92{\times}10^5cells\;mL^{-1}$로 가장 낮았다. 따라서, 녹말과 알긴산의 혼합으로 구성된 피막에서 녹말의 함량이 증가할수록 미세조류 및 해조류 유주자의 생존 및 생장율은 증가되지만 알긴산이 50% 이하인 배합비에서는 물성이 약한 것으로 나타났다. 또한, 인공어초 표면에 응용한 결과, 99 개체 $cm^{-2}$가 발아하는 것으로 나타났다. 본 연구는 해양생태계 복원을 위한 해조류 유주자의 생존율 및 생장률을 향상 시킬 수 있는 다양한 기초자료가 될 것으로 사료되어진다.

Keywords

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