Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Isolation and Characterization of Thermophilic Bacillus licheniformis YJ09 from Spent Mushroom (Pleurotus ostreatus) Substrates

느타리 수확후배지로부터 분리된 고온성 Bacillus licheniformis YJ09의 특성

  • Kim, Hye Soo (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Kim, Chul Hwan (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Cho, Soo Jeong (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 김혜수 (경남과학기술대학교 재약공학과) ;
  • 김철환 (경남과학기술대학교 재약공학과) ;
  • 조수정 (경남과학기술대학교 재약공학과)
  • Received : 2016.12.05
  • Accepted : 2016.12.19
  • Published : 2016.12.31
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Abstract

In order to isolate thermophilic bacteria with high activity of CMCase and xylanase, spent mushroom substrates was collected from an oyster mushroom cultivation farm in Jinju, Gyeongnam, Korea. Among the isolates, one strain designated as YJ09 was selected by agar diffusion method. The isolate YJ09 was identified as a member of Bacillus licheniformis based on biochemical characteristics using Bacillus ID kit and MicroLog system. Comparative 16S rDNA sequence analysis showed that isolate YJ09 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus licheniformis with sequence similarity of 98.9%. Based on its physiological properties, biochemical characteristics and phylogenetic distinctiveness, the isolate YJ09 was classified as Bacillus licheniformis. The CMCase and xylanase activity of B. licheniformis YJ09 was slightly increased corresponding to the bacterial population from exponential phase to stationary phase in the growth curve of B. licheniformis YJ09.

CMCase와 xylanase 분비능이 우수한 고온성 세균을 분리하기 위하여 진주 인근지역의 느타리 재배농장으로부터 수확후배지를 수집하였다. 느타리 수확후배지로부터 17종의 균주를 분리하였으며 이 중 CMCase와 xylanase 활성이 큰 균주 YJ09를 선발하였다. Bacillus ID kit와 MicroLog system을 이용하여 분리균 YJ09의 생리적 생화학적 특성을 조사한 결과 분리균 YJ09은 B. licheniformis와 유사한 특징을 나타내었으며 16S rDNA 염기서열 분석 결과에서도 B. licheniformis와 98.9%의 상동성을 나타내었다. 이와 같은 결과를 종합하여 분리균 YJ09은 B. licheniformis YJ09로 동정되었다. 분리균이 분비하는 CMCase와 xylanase 활성은 분리균이 증식함에 따라 대수증식기 중반부터 급격히 증가하였고 정지기에 진입하면 효소활성이 더 이상 증가하지 않는 것으로 나타났으며 xylanase 활성은 대수증식기 초기부터 지속적으로 증가하여 대수증식기 중반에 최대활성을 나타내었다.

Keywords

References

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