Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Antiplatelet Effects of Cordycepin-Enriched WIB-801CE from Cordyceps militaris: Involvement of Thromboxane A2, Serotonin, Cyclooxygenase-1, Thromboxane A2 Synthase, Cytosolic Phospholipase A2

  • Ok, Woo Jeong (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University) ;
  • Nam, Gi Suk (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University) ;
  • Kim, Min Ji (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University) ;
  • Kwon, Hyuk-Woo (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University) ;
  • Kim, Hyun-Hong (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University) ;
  • Shin, Jung-Hae (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University) ;
  • Lim, Deok Hwi (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University) ;
  • Kwon, Ho-Kyun (Central Research Center, Whanin Pharm. Co., Ltd.) ;
  • Lee, Chang-Hwan (Central Research Center, Whanin Pharm. Co., Ltd.) ;
  • Chung, Soo-Hak (Central Research Center, Whanin Pharm. Co., Ltd.) ;
  • Kim, Jong-Lae (Central Research Center, Whanin Pharm. Co., Ltd.) ;
  • Park, Hwa-Jin (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University)
  • Received : 2016.09.04
  • Accepted : 2016.11.14
  • Published : 2016.12.31
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Abstract

A species of the fungal genus Cordyceps has been used as an ingredient of traditional Chinese medicine. In this study, we prepared cordycepin-enriched WIB-801CE, an ethanol extract from culture solution of Cordyceps militaris-hypha, and evaluated its antiplatelet effects on human platelet aggregation. WIB-801CE dose-dependently inhibited ADP-, collagen-, and thrombin-induced platelet aggregation. These antiplatelet effects by WIB-801CE were associated with the attenuation of thromboxane $A_2$ ($TXA_2$) production and serotonin release by ADP, collagen, and thrombin. The inhibition of $TXA_2$ production by WIB-801CE was due to the inhibition of cyclooxygenase-1, $TXA_2$ synthase, and cytosolic phospholipase $A_2$ activity. Therefore, these data suggest that WIB-801CE may be a beneficial component against protection from platelet aggregation-mediated thrombotic disease.

Keywords

References

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