한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제33권6호
  • /
  • Pages.346-350
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  • 2016
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지
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PCR-based Detection of Bovine Papillomavirus DNA from the Cutaneous Papillomas and Surrounding Environments in the Korean Native Cattle, Hanwoo

  • Han, Sang-Hyun (Educational Science Research Institute, Jeju National University) ;
  • Park, Yong-Sang (Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University) ;
  • Seo, Jong-Pil (Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University) ;
  • Kang, Tae-Young (Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University)
  • 투고 : 2016.05.17
  • 심사 : 2016.08.17
  • 발행 : 2016.12.30
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초록

Two 1-year old calves of Korean Native cattle (Hanwoo) presented cutaneous papillomas on the face and neck. Type 2 bovine papillomavirus (BPV-2) was identified in the cutaneous papillomas based on BPV-specific PCR and subsequent DNA sequencing analysis results. Using DNA samples extracted from two affected calves and unaffected animals reared in the same stable, BPV-2 was not only detected in the cutaneous papillomas of affected animals based on BPV-specific PCR analysis, but also detected in normal skins, hairs, and their environments based on nested PCR analysis. BPV-2 was also detected in DNA samples isolated from animals and environments of that distinct stable with affected calves. However, no BPV-2 was detected in the drinking water of both stables (infected and unaffected). These findings concluded that BPV-2 was transmitted by direct or indirect contact, not by drinking water. This is the first report to show molecular evidence of BPV-2 infection. Rapid and precise molecular identification can be used to screen BPV-2 in cattle farms to understand the biological roles of BPV in animal diseases.

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