Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지
DOI QR코드

DOI QR Code

Protective Effects of Blue Honeysuckle on Rat Hypothyroidism Induced by Propylthiouracil

  • Lee, Woo-Yeol (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Yi, Seong-Joon (Department of Veterinary Anatomy, College of Veterinary Medicine, Kyungpook National University) ;
  • Yun, Sungho (Department of Veterinary Surgery, College of Veterinary Medicine, Kyungpook National University) ;
  • Lim, Mee-Kyung (School of Healthcare and Biotechnology, Kyungwoon University) ;
  • Lee, Keun-Woo (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University)
  • Received : 2016.07.04
  • Accepted : 2016.09.21
  • Published : 2016.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of the present study is to determine whether blue honeysuckle lyophilized concentrated powder (BH) has favorable effects on hypothyroidism and related reproductive organ damage. Hypothyroidism was induced by 9 subcutaneous administration of propylthiouracil (PTU) for 28 days. Levothyroxine (LT4)-treated group was intraperitoneally injected with LT4 for the same period, while for BH (125, 250, and 500 mg/kg) or Flos Lonicerae lyophilized aqueous extract (LF, 250 mg/kg)-treated groups, the test materials were orally administrated for 42 days: two weeks before PTU injection and during PTU administration. The changes in serum thyroid hormone levels, serum male sex hormone levels, and testis antioxidant defense system were observed by histopathology of the thyroid gland, epididymis, prostate, and testis. The oral administrations of 125, 250, and 500 mg/kg of BH showed favorable effects compared to LF on hypothyroidism and related damages of reproductive organs through augmentation of the antioxidant defense system in the testis. In conclusion, BH is a promising new potent thyroid gland protecting agent.

Keywords

References

  1. Aitken RJ. A Free-Radical Theory of Male-Infertility. Reprod Fertil Dev 1994; 6: 19-24. https://doi.org/10.1071/RD9940019
  2. Brown RS. Autoimmune Thyroiditis in Childhood. J Clin Res Pediatr Endocrinol 2013; 5: 45-49.
  3. Chakera AJ, Pearce SHS, Vaidya B. Treatment for primary hypothyroidism: current approaches and future possibilities. Drug Des Devel Ther 2011; 6: 1-11.
  4. Chaovanalikit A, Thompson MM, Wrolstad RE. Characterization and quantification of Anthocyanins and polyphenolics in blue honeysuckle (Lonicera caerulea L.). J Agric Food Chem 2004; 52: 848-852. https://doi.org/10.1021/jf030509o
  5. Chen L, Xin XL, Yuan QP, Su DH, Liu W. Phytochemical properties and antioxidant capacities of various colored berries. J Sci Food Agric 2014; 94: 180-188. https://doi.org/10.1002/jsfa.6216
  6. Choudhury S, Chainy GBN, Mishro MM. Experimentally induced hypo- and hyper-thyroidism influence on the antioxidant defence system in adult rat testis. Andrologia 2003; 35: 131-140. https://doi.org/10.1046/j.1439-0272.2003.00548.x
  7. Clermont Y. The cycle of the seminiferous epithelium in man. Am J Anat 1963; 112: 35-51. https://doi.org/10.1002/aja.1001120103
  8. Cooke PS, Hess RA, Porcelli J, Meisami E. Increased sperm production in adult rats after transient neonatal hypothyroidism. Endocrinology 1991; 129: 244-248. https://doi.org/10.1210/endo-129-1-244
  9. Devdhar M, Ousman YH, Burman KD. Hypothyroidism. Endocrinol Metab Clin North Am 2007; 36: 595-615. https://doi.org/10.1016/j.ecl.2007.04.008
  10. Gartner LP, Hiatt JL. Male reproductive system. In: Color textbook of histology, 3rd ed. Philadelphia: Saunders/Elsevier. 2007: 489-510.
  11. Guyton AC. Thyroid Metabolic Hormones. In: Textbook of medical physiology. 11th ed. Philadelphia: Saunders. 2006: 931-943.
  12. Heinrich J, Valentova K, Vacek J, Palikova I, Zatloukalova M, Kosina P, Ulrichova J, Vrbkova J, Simanek V. Metabolic Profiling of Phenolic Acids and Oxidative Stress Markers after Consumption of Lonicera caerulea L. Fruit. J Agric Food Chem 2013; 61: 4526-4532. https://doi.org/10.1021/jf304150b
  13. Jannini EA, Ulisse S, Darmiento M. Thyroid-Hormone and Male Gonadal-Function. Endocr Rev 1995; 16: 443-459.
  14. Jin XH, Ohgami K, Shiratori K, Suzuki Y, Koyama Y, Yoshida K, Ilieva I, Tanaka T, Onoe K, Ohno S. Effects of blue honeysuckle (Lonicera caerulea L.) extract on lipopolysaccharide-induced inflammation in vitro and in vivo. Exp Eye Res 2006; 82: 860-867. https://doi.org/10.1016/j.exer.2005.10.024
  15. Jurgonski A, Juskiewicz J, Zdunczyk Z. An anthocyaninrich extract from Kamchatka honeysuckle increases enzymatic activity within the gut and ameliorates abnormal lipid and glucose metabolism in rats. Nutrition 2013; 29: 898-902. https://doi.org/10.1016/j.nut.2012.11.006
  16. Jurikova T, Rop O, Mlcek J, Sochor J, Balla S, Szekeres L, Hegedusova A, Hubalek J, Adam V, Kizek R. Phenolic Profile of Edible Honeysuckle Berries (Genus Lonicera) and Their Biological Effects. Molecules 2012; 17: 61-79.
  17. Khandelwal D, Tandon N. Overt and Subclinical Hypothyroidism Who to Treat and How. Drugs 2012; 72: 17-33. https://doi.org/10.2165/11598070-000000000-00000
  18. Ku SK, Seo BI, Park JH, Park GY, Seo YB, Kim JS, Lee HS, Roh SS. Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity. World J Gastroenterol 2009; 15: 4799-4805. https://doi.org/10.3748/wjg.15.4799
  19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-275.
  20. Mutvei A, Nelson BD. The response of individual polypeptides of the mammalian respiratory chain to thyroid hormone. Arch Biochem Biophys 1989; 268: 215-220. https://doi.org/10.1016/0003-9861(89)90582-1
  21. Oppenheimer JH, Schwartz HL, Mariash CN, Kinlaw WB, Wong NC, Freake HC. Advances in our understanding of thyroid hormone action at the cellular level. Endocr Rev 1987; 8: 288-308. https://doi.org/10.1210/edrv-8-3-288
  22. Palikova I, Valentova K, Oborna I, Ulrichova J. Protectivity of blue honeysuckle extract against oxidative human endothelial cells and rat hepatocyte damage. J Agric Food Chem 2009; 57: 6584-6589. https://doi.org/10.1021/jf9003994
  23. Peltola V, Huhtaniemi I, Ahotupa M. Antioxidant enzyme activity in the maturing rat testis. J Androl 1992; 13: 450-455.
  24. Sahoo DK, Roy A, Chainy GBN. Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress. Chem Biol Interact 2008; 176: 121-128. https://doi.org/10.1016/j.cbi.2008.07.009
  25. Sahoo DK, Roy A, Bhanja S, Chainy GB. Hypothyroidism impairs antioxidant defence system and testicular physiology during development and maturation. Gen Comp Endocrinol 2008; 156: 63-70. https://doi.org/10.1016/j.ygcen.2007.11.007
  26. Sanocka D, Miesel R, Jedrzejczak P, Kurpisz MK. Oxidative stress and male infertility. J Androl 1996; 17: 449-454.
  27. Schmidt RF and Thews G. Endocrinology. In: Human physiology, 2nd ed. New York: Springer-Verlag. 1989: 385-386.
  28. So M, MacIsaac RJ, Grossmann M. Hypothyroidism Investigation and management. Aust Fam Physician 2012; 41: 556-562.
  29. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497-500.
  30. Svarcova I, Heinrich J, Valentova K. Berry fruits as a source of biologically active compounds: the case of Lonicera caerulea. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2007; 151: 163-174. https://doi.org/10.5507/bp.2007.031
  31. Svobodova A, Rambouskova J, Walterova D, Vostalova J. Protective effects of phenolic fraction of blue honeysuckle fruits against UVA-induced damage to human keratinocytes. Arch Dermatol Res 2008; 300: 225-233. https://doi.org/10.1007/s00403-008-0850-5
  32. Verhoeven AJ, Kamer P, Groen AK, Tager JM. Effects of thyroid hormone on mitochondrial oxidative phosphorylation. Biochem J 1985; 226: 183-192. https://doi.org/10.1042/bj2260183
  33. Zhao HT, Wang ZY, Ma FM, Yang X, Cheng CL, Yao L. Protective Effect of Anthocyanin from Lonicera Caerulea var. Edulis on Radiation-Induced Damage in Mice. Int J Mol Sci 2012; 13: 11773-11782. https://doi.org/10.3390/ijms130911773

Cited by

  1. Lonicera caerulea: An updated account of its phytoconstituents and health-promoting activities vol.107, pp.None, 2021, https://doi.org/10.1016/j.tifs.2020.08.013