The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Protective Effect of PineXol® on Hydrogen Peroxide-induced Apoptosis on SK-N-MC Cells and Focal Ischemia Rodent Models

파인엑솔이 과산화수소로 유도된 SK-N-MC 세포와 뇌졸중 백서 모델에서의 보호효과

  • 홍순오 (서울의료원 신경외과) ;
  • 한경훈 (서울의료원 의학연구소) ;
  • 이승희 (서울의료원 의학연구소) ;
  • 김도희 (서울의료원 의학연구소) ;
  • 송관영 (서울의료원 신경외과) ;
  • 한성희 (고려대학교 보건과학대학 생물신소재연구소)
  • Received : 2016.10.31
  • Accepted : 2016.12.06
  • Published : 2016.12.31
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Abstract

The purpose of this study was to evaluate the protective effect of $PineXol^{(R)}$ on $H_2O_2$-induced cell death in SK-N-MC cells, and in early stage focal ischemia rodent model. SK-N-MC cells were pre-treated with $200{\mu}M$ $H_2O_2$ or various concentrations of $PineXol^{(R)}$ (10, 30, and 50 pg/mL) for 24 h, and then exposed to $H_2O_2$ for 3 h. Cell death was assessed by the CCK-8 assay, reactive oxygen species (ROS) assay, and lactate and dehydrogenase (LDH) release assay. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) expressions were also analyzed by western blotting. Focal ischemia rodent model was used as the in vivo model, and different concentrations of $PineXol^{(R)}$ (1, 10, and 100 mg/kg) were administered. One week after administration, reduction of infarct volume was analyzed by TTC staining. Cell viability of $H_2O_2$-treated SK-N-MC cells significantly increased by pre-treatment of $PineXol^{(R)}$ (p<0.05). $PineXol^{(R)}$ pre-treatment also induced significant decrease of ROS and LDH expressions. However, $PineXol^{(R)}$ did not affect the infarct volume. These results suggest that $PineXol^{(R)}$ has significant neuroprotective effect in vitro, but statistical significance was not confirmed in the in vivo focal ischemia model.

Keywords

References

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