Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Application of sweet and taste modifying genes for development in plants: current status and prospects

  • Akter, Shahina (Department of Horticultural Life Science, Hankyong National University, Institute of Genetic Engineering, Hankyong National University) ;
  • Huq, Md. Amdadul (Department of Horticultural Life Science, Hankyong National University, Institute of Genetic Engineering, Hankyong National University) ;
  • Jung, Yu-Jin (Department of Horticultural Life Science, Hankyong National University, Institute of Genetic Engineering, Hankyong National University) ;
  • Cho, Yong-Gu (Department of Crop Science, Chungbuk National University) ;
  • Kang, Kwon-Kyoo (Department of Horticultural Life Science, Hankyong National University, Institute of Genetic Engineering, Hankyong National University)
  • Received : 2016.12.12
  • Accepted : 2016.12.20
  • Published : 2016.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Sweet and taste modifying proteins are natural alternatives to synthetic sweeteners and flavor enhancers, and have been used for centuries in different countries. Use of these proteins is limited due to less stability and availability. However, recent advances in biotechnology have enhanced their availability. These include production of sweet and taste modifying proteins in transgenic organisms, and protein engineering to improve their stability. Their increased availability in the food, beverage or medicinal industries as sweeteners and flavor enhancers will reduce the dependence on artificial alternatives. Production of transgenic plants using sweet and taste modifying genes, is an interesting alternative to the extraction of these products from natural source. In this review paper, we briefly describe various sweet and taste modifying proteins (such as thaumatin, monellin, brazzein, curculin and miraculin), their properties, and their application for plant development using biotechnological approaches.

Keywords

References

  1. Matsuyama T, Satoh M, Nakata R, Aoyama T, Inoue H (2009) Functional expression of miraculin, a taste-modifying protein in Escherichia coli. J Biochem Apr 145:445-50 https://doi.org/10.1093/jb/mvn184
  2. Anonymous (1969) Ban on Cyclamate Sweeteners. Lancet 2:941-942
  3. Assadi-Porter F, Patryb S, Markleya JL (2008) Efficient and rapid protein expression and purification of small high disulfide containing sweet protein brazzein in E. coli. Protein Expr Purif 58:263-268 https://doi.org/10.1016/j.pep.2007.11.009
  4. Assadi-Porter FM, Aceti D, Markley JL (2000) Sweetness determinant sites of brazzein, a small, heat-stable, sweettasting protein. Arch Biochem Biophys 376:259-265 https://doi.org/10.1006/abbi.2000.1726
  5. Assadi-Porter FM, Aceti DJ, Cheng H, Markley JL (2000) Efficient production of recombinant brazzein, a small, heat-stable, sweet tasting protein of plant origin. Arch Biochem Biophys 376:252-258 https://doi.org/10.1006/abbi.2000.1725
  6. Baneyx F (1999) Recombinant protein expression in Escherichia coli. Curr Opin Biotechnol 10:411-421 https://doi.org/10.1016/S0958-1669(99)00003-8
  7. Bartoszewski G, Niedziela A, Szwacka M, Niemirowicz-Szczytt K (2003) Modification of tomato taste in transgenic plants carrying a thaumatin gene from Thaumatococcus daniellii Benth Plant Breeding 122:347-351 https://doi.org/10.1046/j.1439-0523.2003.00864.x
  8. Berlec A, Jevnikar Z, Majhenic AC, Rogelj I, Strukelj B (2006) Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis: a path toward sweet lactic acid bacteria. Appl Microbiol Biotechnol 73:158-165 https://doi.org/10.1007/s00253-006-0438-y
  9. Berlec A, Strukelj B (2009) Large increase in brazzein expression achieved by changing the plasmid/strain combination of the NICE system in Lactococcus lactis. Lett Appl Microbiol 48:750-755
  10. Bernard F, Gibbs, Alli I, Mulligan C 1996 Sweet and taste-modifying proteins: a review. Nutrition Research 16:1619-1630 https://doi.org/10.1016/0271-5317(96)00175-3
  11. Bernard G, Niedziela A, Szwacka M, Niemirowicz-Szczytt K (2003) Modification of tomato taste in transgenic plants carrying a thaumatin gene from Thaumatococcus daniellii Benth. Plant Breeding 122:347-351 https://doi.org/10.1046/j.1439-0523.2003.00864.x
  12. Caldwell JE, Abildgaard F, Dzakula Z, Ming D, Hellekant G, Markley JL (1998a) Solution structure of the thermostable sweet-tasting protein brazzein. Nat Struct Biol 5:427-431 https://doi.org/10.1038/nsb0698-427
  13. Caldwell JE, Abildgaard F, Ming D, Hellekant G, Markley JL (1998b) Complete 1H and partial 13C resonance assignments at 37 and 22 degrees C for brazzein, an intensely sweet protein. J Biomol NMR 11:231-232 https://doi.org/10.1023/A:1008262104643
  14. Chen Z, Cai H, Lu F, Du L (2005) High-level expression of a synthetic gene encoding a sweet protein, monellin, in Escherichia coli. Biotechnol Lett 27:1745-1749 https://doi.org/10.1007/s10529-005-3544-5
  15. Daniell S, Mellits KH, Faus I, Connerton I (2000) Refolding the sweet-tasting protein thaumatin II from insoluble inclusion bodies synthesised in Escherichia coli. Food Chem 71:105-110 https://doi.org/10.1016/S0308-8146(00)00151-5
  16. Datta K, Velazhahan R, Oliva N, Ona I, Mew T, Khush GS, Muthukrishnan S, Datta SK (1999) Overexpression of cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease. Theor Appl Genet 98:1138-1145 https://doi.org/10.1007/s001220051178
  17. Edens L, Bom L, Ledeboer AM, Maat J, Toonen MY, Visser C, Verrips CT (1984) Synthesis and processing of the plant protein thaumatin in yeast. Cell 37:629-633 https://doi.org/10.1016/0092-8674(84)90394-5
  18. Edens L, Heslinga L, Klok R, Ledeboer AM, Maat J, Toonen MY, Visser C, Verrips CT (1982) Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Escherichia coli. Gene 18:1-12 https://doi.org/10.1016/0378-1119(82)90050-6
  19. Etheridge K 1994. The sales and marketing of talin. In: Witty M, Higginbotham JD, editors. Boca Raton FL: CRC Press. 47-59
  20. Faus I (2000) Recent developments in the characterization and biotechnological production of sweet-tasting proteins. Appl Microbiol Biotechnol 53:145-151 https://doi.org/10.1007/s002530050001
  21. Faus I, del Moral C, Adroer N, del Rio JL, Patino C, Sisniega H, Casas C, Blade J, Rubio V (1998) Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori. Appl Microbiol Biotechnol, 49:393-398 https://doi.org/10.1007/s002530051188
  22. Faus I, Patino C, del Rio JL, del Moral C, Barroso HS, Blade J, Rubio V (1997) Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in the filamentous fungus Penicillium roquefortii. Biotechnol Lett 19:1185-1191 https://doi.org/10.1023/A:1018477602350
  23. Faus I, Patino C, del Rio JL, del Moral C, Barroso HS, Rubio V (1996) Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in Escherichia coli. Biochem Biophys Res Commun 229:121-127 https://doi.org/10.1006/bbrc.1996.1767
  24. Gibbs BF, Alli I, Mulligan C (1996) Sweet and taste-modifying proteins: a review. Nutr Res 16:1619-1630 https://doi.org/10.1016/0271-5317(96)00175-3
  25. Guan Z, Hellekant G, Yan W (1995) Expression of sweet protein brazzein by Saccharomyces cerevisiae. Chem Senses 20:701
  26. Hahm YT, Batt CA (1990) Expression and secretion of thaumatin from Aspergillus oryzae. Agric Biol Chem 54:2513-2520
  27. Hannig G, Makrides SC (1998) Strategies for optimizing heterologous protein expression in Escherichia coli. Trends Biotechnol 16:54-60 https://doi.org/10.1016/S0167-7799(97)01155-4
  28. Higginbottom HJD (1985) Food Flavourings, Ingredients, Processing & Packaging. Talin update 3:39
  29. Hirai T, Kim YW, Kato K, Hiwasa-Tanase K, Ezura H (2011) Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit ripening-specific E8 promoter. Transgenic Res 20:1285-1292 https://doi.org/10.1007/s11248-011-9495-9
  30. Illingworth C, Larson G, Hellekant G (1988) Secretion of the sweet-tasting plant protein thaumatin by Bacillus subtilis. Biotechnol Lett 10:587-592 https://doi.org/10.1007/BF01027135
  31. Illingworth C, Larson G, Hellekant G (1989) Secretion of the sweet-tasting plant protein thaumatin by Streptomyces lividans. J Ind Microbiol 4:37-42 https://doi.org/10.1007/BF01569691
  32. Jo HJ, Noh JS, Kong KH (2013) Efficient secretory expression of the sweet-tasting protein brazzein in the yeast Kluyveromyces lactis. Protein Expression and Purification 90:84-89 https://doi.org/10.1016/j.pep.2013.05.001
  33. Kant R (2005) Sweet proteins, potential replacement for artificial low calorie sweeteners. Nutr J 4:5 https://doi.org/10.1186/1475-2891-4-5
  34. Kim SH, Kang CH, Kim R, Cho JM, Lee YB, Lee TK (1989) Redesigning a sweet protein: increased stability and renaturability. Protein Eng 2:571-5 https://doi.org/10.1093/protein/2.8.571
  35. Kohmura M, Nio N, Ariyoshi Y, Complete amino acid sequence of the sweet protein monellin. Agricultural and Biological Chemistry 54:2219-2224
  36. Kondo K, Miura Y, Sone H, Kobayashi K, Iijima H (1997) High-level expression of a sweet protein, monellin, in the food yeast Candida utilis. Nat Biotechnol 15:453-457 https://doi.org/10.1038/nbt0597-453
  37. Kurihara K, Beidler LM (1969) Mechanism of the action of taste-modifying protein. Nature 222:1176-1179 https://doi.org/10.1038/2221176a0
  38. Kurihara Y, Beidler LM (1968) Taste-modifying protein from miracle fruit. Science 161:1241-1243 https://doi.org/10.1126/science.161.3847.1241
  39. Kurihara Y, Nirasawa S (1997) Structures and activities of sweetness-inducing substances (miraculin, curculin, strogin) and the heat-stable sweet protein, mabinlin. FFI J Jpn 174:67-74
  40. Lamphear BJ, Barker DK, Brooks CA, Delaney DE, Lane JR, Beifuss K, Love R, Thompson K, Mayor J, Clough R (2005) Expression of the sweet protein brazzein in maize for production of a new commercial sweetener. Plant Biotechnol J 3:103-114
  41. Lebedev VG, Taran SA, Shmatchenko VV, Dolgov SV (2002) Pear transformation with the gene for super sweet protein thaumatin II. Acta Hortic 596:199-202
  42. Lee JJ, Kong JN, Do HD, Jo DH, Kong KH (2010) Design and efficient soluble expression of a sweet protein, brazzein and minor-form mutant. Bull Korean Chem Soc 31:3830-3833 https://doi.org/10.5012/bkcs.2010.31.12.3830
  43. Liu J, Yana DZ, Zhaob SJ (2015) Expression of monellin in a food-grade delivery system in Saccharomyces cerevisiae. J Sci Food Agric 95:2646-51 https://doi.org/10.1002/jsfa.6997
  44. Liu Q, Li L, Yang L, Liu T, Cai C, Liu B (2016) Modification of the Sweetness and Stability of Sweet-Tasting Protein Monellin by Gene Mutation and Protein Engineering. BioMed Research International 2016:3647173
  45. Makrides SC (1996) Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol Rev 60:512-538
  46. Masuda M, Tamaki S, Kaneko R, Wada R, Fujita Y, Mehta A, Kitabatake N (2004) Cloning, Expression, and Characterization of Recombinant Sweet-Protein Thaumatin II Using the Methylotrophic Yeast Pichia pastoris. Biotechnol Bioeng 85:761-769 https://doi.org/10.1002/bit.10786
  47. Masuda T, Kitabatake N (2006) Developments in biotechnological production of sweet proteins. J Biosci Bioeng 102:375-389 https://doi.org/10.1263/jbb.102.375
  48. Ming D, Hellekant G (1994) Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B. FEBS Lett 355:106-108 https://doi.org/10.1016/0014-5793(94)01184-2
  49. Moralejo FJ, Cardoza RE, Gutierrez S, Martin JF (1999) Thaumatin production in Aspergillus awamori by use of expression cassettes with strong fungal promoters and high gene dosage. Appl Environ Microbiol 65:1168-1174
  50. Moris JA, Cagan RH (1972) Purification of monellin, the sweet principal for Dioscoreophyllum cumminsii. Biochim Biophys Acta 261:114-122 https://doi.org/10.1016/0304-4165(72)90320-0
  51. Morris JA, Cagan RH (1973) Chemostimulatory protein: a new type of taste stimulus. Science 181:32-35 https://doi.org/10.1126/science.181.4094.32
  52. Penarrubia L, Kim R, Giovannoni J, Kim SH, Fischer RL (1992) Production of the sweet protein monellin in transgenic plants. Nature Biotechnol 10:561-564 https://doi.org/10.1038/nbt0592-561
  53. Price JM, Biava CG, Oser BL, Vogin EE, Steinfield J, Ley HL (1970) 'Bladder tumors in rats fed cyclohexylamine or high doses of a mixture of cyclamate and saccharin'. Science 167:1131-1132 https://doi.org/10.1126/science.167.3921.1131
  54. Reddy CS, Vijayalakshmi M, Kaul T, Islam T, Reddy MK (2015) Improving flavour and quality of tomatoes by expression of synthetic gene encoding sweet protein monellin. Mol Biotechnol 57:448-453 https://doi.org/10.1007/s12033-015-9838-5
  55. Schestibratov KA, Dolgov SV (2005) Transgenic strawberry plants expressing a thaumatin II gene demonstrate enhanced resistance to Botrytis cinerea. Sci Hortic 106:177-189 https://doi.org/10.1016/j.scienta.2005.03.016
  56. Sugaya T, Yano M, Sun HJ, Hirai T, Ezura H (2008) Transgenic strawberry expressing a taste modifying protein, miraculin. Plant Biotechnol 25:329-333 https://doi.org/10.5511/plantbiotechnology.25.329
  57. Sun HJ, Cui ML, Ma B, Ezura H (2006) Functional expression of the taste-modifying protein, miraculin, in transgenic lettuce. FEBS Lett 580:620-626 https://doi.org/10.1016/j.febslet.2005.12.080
  58. Sun HJ, Kataoka H, Yano M, Ezura H (2007) Genetically stable expression of functional miraculin, a new type of alternative sweetener, in transgenic tomato plants. Plant Biotechnol J 5:768-777 https://doi.org/10.1111/j.1467-7652.2007.00283.x
  59. Suzuki M, Kurimoto E, Nirasawa S, Masuda Y, Hori K, Kurihara Y, Shimba N, Kawai M, Suzuki E, Kato K (2004) Recombinant curculin heterodimer exhibits taste-modifying and sweet-tasting activities. FEBS Lett 573:135-8 https://doi.org/10.1016/j.febslet.2004.07.073
  60. Szwacka M, Kryzymowska M, Osuch A, Kowalczyk ME, Malepszy S (2002) Variable properties of transgenic cucumber plants containing the thaumatin II gene from Thaumatococcus daniellii. Acta Physiol Plant 24:173-185 https://doi.org/10.1007/s11738-002-0009-5
  61. Tancredi T, Iijima H, Saviano G, Amodeo P, Temussi PA (1992) Structural determination of the active site of a sweet protein. A1H NMR investigation of pMNEI. FEBS Letters 310:27-30 https://doi.org/10.1016/0014-5793(92)81138-C
  62. Theerasilp S, Kurihara Y (1988) Complete purification and characterization of the taste-modifying protein, miraculin, from miracle fruit. J Biol Chem 263:11536-11539
  63. Van der Wel H (1972) Isolation and characterization of the sweet principal for Dioscoreophyllum cumminsii (Stapf) Diels. FEBS Lett 21:88-90 https://doi.org/10.1016/0014-5793(72)80170-4
  64. Van der Wel H, Loeve K (1972) Isolation and characterization of thaumatin I and II, the sweet-tasting proteins from Thaumatococcus daniellii Benth. Eur J Biochem 31:221-225 https://doi.org/10.1111/j.1432-1033.1972.tb02522.x
  65. Van Loon LC (1997) Induced resistance in plants and the role of pathogenesis-related proteins. Eur J Plant Path 103:753-765 https://doi.org/10.1023/A:1008638109140
  66. Weickmann JL, Blair LC, Wilcox GL (1994). High level expression of thaumatin in Saccharomyces cerevisiae. P:151-169. In Witty, M. and Higginbotham, J. D. (ed.), Thaumatin. CRC Press, Boca Raton, FL
  67. Witty M (1990) Preprothaumatin II is processed to biological activity in Solanum tuberosum. Biotechnol Lett 12:131-136 https://doi.org/10.1007/BF01022429
  68. Witty M (1998) New technologies for taste modifying proteins. Trends Food Sci Technol 9:275:280 https://doi.org/10.1016/S0924-2244(98)00048-X
  69. Witty M, JD (1994) Higginbotham (eds), Thaumatin. CRC Press Inc. Boca Raton
  70. Yamashita H, Theerasilp S, Aiuchi T, Nakaya K, Nakamura Y, Kurihara Y (1990) Purification and complete amino acid sequence of a new type of sweet protein with taste-modifying activity, curculin. J Biol Chem 265:15770-15775
  71. Yan S, Song H, Pang D, Zou Q, Li L, Yan Q, Fan N, Zhao X, Yu H, Li Z, Wang H, Gao F, Ouyang H, Lai L (2013) Expression of Plant Sweet Protein Brazzein in the Milk of Transgenic Mice. PLoS One 8:e76769 https://doi.org/10.1371/journal.pone.0076769
  72. Zhu B, Chen THH, Li PH (1996) Analysis of late-blight disease resistance and freezing tolerance in transgenic potato plants expressing sense and antisense genes for an osmotin-like protein. Planta 198:70-77

Cited by

  1. Stable expression of brazzein protein, a new type of alternative sweetener in transgenic rice vol.45, pp.1, 2018, https://doi.org/10.5010/JPB.2018.45.1.063