Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Marker-Assisted Selection for Monoecy in Chamoe (Cucumis melo L.)

성발현 연관 분자마커를 이용한 단성화 참외 선발

  • Bang, Sun-Woong (Department of Life Science, Dongguk University-Seoul) ;
  • Song, Kihwan (Department of Bioresources Engineering, Sejong University) ;
  • Sim, Sung Chur (Department of Bioresources Engineering, Sejong University) ;
  • Chung, Sang Min (Department of Life Science, Dongguk University-Seoul)
  • 방선웅 (동국대학교 생명과학과) ;
  • 송기환 (세종대학교 식물생명공학전공) ;
  • 심성철 (세종대학교 식물생명공학전공) ;
  • 정상민 (동국대학교 생명과학과)
  • Received : 2015.08.21
  • Accepted : 2015.09.24
  • Published : 2016.02.29
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Abstract

The DNA marker T1ex, originally developed from melon (Cucumis melo L.) for monoecy, was employed in chamoe, which is referred to as oriental melon. This marker shows size variations in monoecious melon. However, in chamoe, no such detrimental size variation was found in monoecious chamoe, and 99% association between flower phenotypes and genotypes of the T1ex marker was observed in 106 lines of chamoe. To evaluate the efficacy of the T1ex marker for marker-assisted selection (MAS), a total of 240 plants of chamoe breeding lines were screened using the T1ex marker. Among these, 98 varieties were selected. Although the T1ex marker might not be useful for MAS in melon, we found 100% concordance between genotypes and phenotypes for sex expression in chamoe. These results suggest that the T1ex marker will be a useful resource for MAS for monoecy in chamoe.

멜론에서 개발된 단성화 연관 마커인 T1ex를 참외계통 선발에 적용하였다. T1ex 마커가 멜론에서는 단성화에 대해 두 가지 크기의 변이를 보였으나 참외에서는 단일 크기 변이를 보였고 하나의 계통을 제외한 나머지 105 계통에서 단성화와 양성화에 대한 표현형과 유전자형이 99% 일치함을 보였다. 또한 T1ex 단성화 연관마커의 MAS 적용가능성을 확인하기 위해 참외 품종 육성에 이용되고 있는 240개 개체 중 분자마커 선택법으로 선발된 98개체를 비교해 본 결과 표현형과 유전자형이 100% 일치하였고, 이형 유전자형을 조기에 효과적으로 제거 할 수 있음을 확인하였다. 따라서 멜론에서는 적용이 어렵다고 판단된 단성화 연관 마커 T1ex가 참외에서는 계통 육성과정에서 적용 가치가 매우 높다고 평가된다.

Keywords

References

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