The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Anti-Helicobacter pylori Activity of Korean Propolis

국산 프로폴리스의 Helicobacter pyrori 억제효과

  • Han, Sang Mi (Dept. of Agricultural Biology, National Academy of Agricultural Science) ;
  • Hong, In Phyo (Dept. of Agricultural Biology, National Academy of Agricultural Science) ;
  • Woo, Soon Ok (Dept. of Agricultural Biology, National Academy of Agricultural Science) ;
  • Kim, Se Gun (Dept. of Agricultural Biology, National Academy of Agricultural Science) ;
  • Jang, Hye Ri (Dept. of Agricultural Biology, National Academy of Agricultural Science) ;
  • Jang, Jae Seon (Dept. of Food & Nutrition, Gachon University)
  • 한상미 (국립농업과학원 농업생물부 잠사양봉소재과) ;
  • 홍인표 (국립농업과학원 농업생물부 잠사양봉소재과) ;
  • 우순옥 (국립농업과학원 농업생물부 잠사양봉소재과) ;
  • 김세건 (국립농업과학원 농업생물부 잠사양봉소재과) ;
  • 장혜리 (국립농업과학원 농업생물부 잠사양봉소재과) ;
  • 장재선 (가천대학교 식품영양학과)
  • Received : 2015.12.24
  • Accepted : 2016.02.22
  • Published : 2016.02.29
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Abstract

Propolis possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. The aim of this study was to evaluated chemical composition and in vitro anti-Helicobacter pylori activity of eight propolis ethanolic extracts form different region in Korea. The chemical composition of eight Korean propolis was investicated by absorption spectrophotomety and UHPLC. Total penolic and total flavonoids content ranged between $21{\pm}1.5$ and $31{\pm}1.8$ gallic acid g/100 g and $7.2{\pm}0.9$ and $24.4{\pm}1.9$ quercetin g/100 g, respectively. The p-coumaric acid as standard components content ranged between $0.29{\pm}0.00$ and $2.34{\pm}0.02mg/g$ using UHPLC. Cinnamic acid content between $0.43{\pm}0.00$ and $1.33{\pm}0.01mg/g$. 80% ethanolic extract of propolis against H. pylori was evaluated by using the disc diffusion method. Ethanol was used as a control. Mean diameters of H. pylori growth inhibition using $10{\mu}g/mL$, $50{\mu}g/mL$ or $100{\mu}g/mL$ ethanol propolis per disc were 0, ranging from 0.9 to 2.1 mm and from 1.9 to 3.5 mm, respectively. In conclusion, variation in the chemical composition of Korean propolis were mainly due to the difference in the preferred regional plants visited by honeybees. Also Korean propolis possesses considerable antibacterial activity against H. pylori. There was not confirmed a correlation between the chemical composion contens and anti-H. pylori effect. The potential of propolis in the prevention or treatment of H. pylori infection is worth further extensive evaluation.

Keywords

References

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