Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Detection of Superior Markers for Polymerase Chain Reaction Diagnosis of Breast Cancer Micrometastasis in Sentinel Lymph Nodes

  • Shargh, Shohreh Alizadeh (Department of Medical Laboratory Science, Midwifery-Nursing Institute, Islamic Azad University of Chalous Branch) ;
  • Movafagh, Abolfazl (Department of Medical Genetics, Pediatric Neurology Research Center, Shahid Beheshti University of Medical Sciences) ;
  • Zarghami, Nosratolah (Department of medical Biotechnology, Tabriz University of Medical Sciences) ;
  • Sayad, Arezou (Department of Medical Genetics, Pediatric Neurology Research Center, Shahid Beheshti University of Medical Sciences) ;
  • Mansouri, Neda (Department of Medical Genetics, Pediatric Neurology Research Center, Shahid Beheshti University of Medical Sciences) ;
  • Taheri, Mohammad (Department of Medical Genetics, Pediatric Neurology Research Center, Shahid Beheshti University of Medical Sciences) ;
  • Pour, Atefeh Heidary (Department of Cell and Molecular Biology, Pharmaceutical Sciences Branch, Islamic Azad University) ;
  • Iranpour, Mostafa (Department of Medical Genetics, Pediatric Neurology Research Center, Shahid Beheshti University of Medical Sciences) ;
  • Ghaedi, Hamid (Department of Medical Genetics, Pediatric Neurology Research Center, Shahid Beheshti University of Medical Sciences) ;
  • Montazeri, Vahid (Department of General Surgery, School of Medicine, Hematology and Oncology Research Center, Tabriz University of Medical Sciences) ;
  • Massoudi, Nilofar (Department of Anesthesiology, School of Medicine, Shahid Beheshti University of Medical Sciences) ;
  • Hashemi, Mehrdad (Department of Genetic, Islamic Azad University) ;
  • Mortazavi-Tabatabaei, SA (Proteomics Research Center, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences)
  • Published : 2016.06.01
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Abstract

Breast cancer is the most prevalent type of cancer among women around the world, and mortality is primarily caused by micro-metastatic disease. The complex mechanisms of breast cancer invasion and metastasis are intrinsically related to the malignant cell type so that early detection of micro-metastases can help prolongation of survival for patient. The aim of the present research work was evaluation of the expression status of mammoglobin protein as a candidate molecular marker in the negative sentinel lymph node (SLN). Fifty tumor specimens, and 50 normal adjacent breast tissue samples from the same patients were selected on the basis of having more than 10% tumor content for RNA extraction from SLNs. Tumor samples and normal adjacent breast tissue were archived in the form of frozen fresh tissue in liquid nitrogen. Real-time PCR was performed on a Bioner life express gradient thermal cycler system. Mammoglobin gene overexpression in breast cancer metastasis was investigated. Single marker results were mammaglobin 66.7% and CK19 50.0%, with 58.3% for the two in combination. Due to improved outcome with at least 3 genes (83.3%), it seems, triple marker evaluation will be most likely useful for detecting micro-metastases instead of studying separate genes.

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References

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