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Comparison of Antioxidant Activities in Tomato Leaves and Stems

  • Lee, Kyung Jun (National Agrobiodiversity Center, NAS, RDA) ;
  • Lee, Gi-An (National Agrobiodiversity Center, NAS, RDA) ;
  • Lee, Jong-Ro (National Agrobiodiversity Center, NAS, RDA) ;
  • Chung, Jong-Wook (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Cho, Yang-Hee (National Agrobiodiversity Center, NAS, RDA) ;
  • Kang, Hee-Kyoung (Department of Horticulture, College of Industrial Science, Kongju National University) ;
  • Ma, Kyung-Ho (National Agrobiodiversity Center, NAS, RDA)
  • Received : 2016.06.07
  • Accepted : 2016.08.26
  • Published : 2016.12.31

Abstract

This study was conducted to investigate the antioxidant activity in the leaves and stems of 50 tomato accessions, in order to examine the possibility of using tomato by-products as a functional material. The extracts of the leaves (LE) and stems (SE) were analyzed for DPPH, ABTS, and total polyphenol content (TPC). Antioxidant activities and TPC differed significantly between the LE and SE of the 50 tomato accessions. TPC in LE and SE showed wide variation, ranging from 24.4 to 60.6 and 12.5 to 18.8 mg GAE/g, respectively. The DPPH and ABTS antioxidant activities of LE ranged from 10.0 to 38.2% (scavenging effect) and 20.8 to 59.0 mg ASC/g, respectively, while the DPPH and ABTS measurements of SE were 1.4 to 8.8% and 2.2 to 22.5 mg ASC/g, respectively. As assessed by the relative antioxidant capacity index (RACI), IT033117 and IT203466 had the highest antioxidant activity in LE and SE, respectively. These results will expand the knowledge of antioxidant activity and provide information on tomato accessions valuable for the development of functional foods and food additives.

Keywords

References

  1. Ajila, C.M. and U.J.S.P. Rao. 2008. Protection against hydrogen peroxide induced oxidative damage in rat erythrocytes by Mangifera indica L. peel extract. Food Chem. Toxicol. 46:303-309. https://doi.org/10.1016/j.fct.2007.08.024
  2. Amaeze, O.U., G.A. Ayoola, M.O. Sofidiya, A.A. Adepoju-Bello, A.O. Adegoke and H.A.B. Coker. 2011. Evaluation of antioxidant activity of Tetracarpidium conophorum (Mull. Arg) Hutch & Dalziel leaves. Oxid. Med. Cell. Longev. Article ID 976701.
  3. Ames, B.N. 1983. Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases. Science 221:1256-1264. https://doi.org/10.1126/science.6351251
  4. Angioni, A., A. Barra, V. Coroneo, S. Dessi and P. Cabras. 2006. Chemical composition, seasonal variability, and antifungal activity of Lavandula stoechas L. ssp. stoechas essential oils from stem/leaves and flowers J. Agr. Food Chem. 54:4364-4370. https://doi.org/10.1021/jf0603329
  5. Chandra, H.M. and S. Ramalingam. 2011. Antioxidant Potentials of Skin, Pulp, and Seed Fractions of Commercially Important Tomato Cultivars. Food Sci. Biotechnol. 20:15-21. https://doi.org/10.1007/s10068-011-0003-z
  6. Chorianopoulos, N., E. Evergets, A. Mallouchos, E. Kalpoutzakis, G.J. Nychas and S.A. Haroutounian. 2006. Characterization of the essential oil volatiles of Satureja thymbra and Satureja parnassica: Influence of harvesting time and antimicrobial activity. J. Agr. Food Chem. 54:3139-3145. https://doi.org/10.1021/jf053183n
  7. Davies, K.J.A. 2000. Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. Iubmb. Life 50:279-289. https://doi.org/10.1080/15216540051081010
  8. Elbadrawy, E. and A. Sello. 2011. Evaluation of nutritional value and antioxidant activity of tomato peel extracts. Arab. J. Chem. (in Press) doi:10.1016/j.arabjc.2011.11.011.
  9. Engelhard, Y.N., B. Gazer and E. Paran. 2006. Natural antioxidants from tomato extract reduce blood pressure in patients with grade-1 hypertension: a double-blind, placebocontrolled pilot study. Am. Heart. J. 151:100.
  10. Finkel, T. and N.J. Holbrook. 2000. Oxidants, oxidative stress and the biology of ageing. Nature 408:239-247. https://doi.org/10.1038/35041687
  11. Frankel, E.N. and A.S. Meyer. 2000. The problems of using one-dimensional methods to evaluate multifunctional food and biological antioxidants. J. Sci. Food Agr. 80:1925-1941. https://doi.org/10.1002/1097-0010(200010)80:13<1925::AID-JSFA714>3.0.CO;2-4
  12. Gey, K.F. 1990. The antioxidant hypothesis of cardiovascular disease: Epidemiology and mechanisms. Biochem. Soc. Trans. 18:1041-1045. https://doi.org/10.1042/bst0181041
  13. Gupta, V.K. and S.K. Sharma. 2006. Plants as natural antioxidants. Nat. Prod. Radiance 5:326-334.
  14. Halliwell, B., M.A. Murcia, S. Chirico and O.I. Aruoma. 1995. Free-radicals and antioxidants in food and in vivo - What they do and how they work. Crit. Rev. Food Sci. 35:7-20. https://doi.org/10.1080/10408399509527682
  15. Hammer, O., D.A.T. Harper and P.D. Ryan. 2001. PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4:1-9.
  16. Khodr, B. and Z. Khalil. 2001. Modulation of inflammation by reactive oxygen species: Implications for aging and tissue repair. Free Radic. Bio. Med. 30:1-8. https://doi.org/10.1016/S0891-5849(00)00378-6
  17. Kim, T.Y., S.A. Jang, Y.B. Chae and J.P. Bak. 2016. Antioxidant and protective effects of Leonurus sibiricus L. extract on ultraviolet B (UVB)-induced damage in human keratinocytes. Korean J. Plant Res. 29:11-19 (in Korean). https://doi.org/10.7732/kjpr.2016.29.1.011
  18. Kim, W.R., E.O. Kim, K. Kang, S. Oidoysambuu, S.H. Jung, B.S. Kim, C.W. Nho and B.H. Um. 2014. Antioxidant activity of phenolics in leaves of three red pepper (Capsicum annuum) Cultivars. J. Agr. Food Chem. 62:850-859. https://doi.org/10.1021/jf403006c
  19. Kobayashi, F., K. Ishida, H. Ikeura, S. Odake and Y. Hayata. 2012. Application of tomato (Solanum lycopersicum) leaf volatiles as antifungal agents against plant pathogenic fungi. J. Agr. Sci. 4: 231-235.
  20. Lee, D.J. and J.Y. Lee. 2004. Antioxidant activity by DPPH assay. Korean J. Crop Sci. 49:187-194 (in Korean).
  21. MacDonald-Wicks, L.K., L.G. Wood and M.L. Garg. 2006. Methodology for the determination of biological antioxidant capacity in vitro: a review. J. Sci. Food Agr. 86:2046-2056. https://doi.org/10.1002/jsfa.2603
  22. Martinez-Valverde, I., M.J. Periago, G. Provan and A. Chesson. 2002. Phenolic compounds, lycopene and antioxidant activity in commercial varieties of tomato (Lycopersicum esculentum). J. Sci. Food Agr. 82:323-330. https://doi.org/10.1002/jsfa.1035
  23. Mossi, A.J., R.L. Cansian, A.Z. Carvalho, C. Dariva, J.V. Oliveira, M. Mazutti, I.N. Filho and S. Echeverrigaray. 2004. Extraction and characterization of volatile compounds in Maytenus ilicifolia, using high-pressure $CO_2$. Fitoterapia 75:168-178. https://doi.org/10.1016/j.fitote.2003.12.006
  24. Nordberg, J. and E.S.J. Arner. 2001. Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radic. Bio. Med. 31:1287-1312. https://doi.org/10.1016/S0891-5849(01)00724-9
  25. Prior, R.L., X.L. Wu and K. Schaich. 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J. Agr. Food Chem. 53:4290-4302. https://doi.org/10.1021/jf0502698
  26. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Bio. Med. 26:1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  27. Rivero, R.M., J.M. Ruiz and L. Romero. 2003. Can grafting in tomato plants strengthen resistance to thermal stress? J. Sci. Food Agr. 83:1315-1319. https://doi.org/10.1002/jsfa.1541
  28. Roginsky, V. and E.A. Lissi. 2005. Review of methods to determine chain-breaking antioxidant activity in food. Food Chem. 92:235-254. https://doi.org/10.1016/j.foodchem.2004.08.004
  29. Silva-Beltran, N.P., S. Ruiz-Cruz, C. Chaidez, J.D. Ornelas-Paz, M.A. Lopez-Mata, E. Marquez-Rios and M.I. Estrada. 2015. Chemical constitution and effect of extracts of tomato plants byproducts on the enteric viral surrogates. Int. J. Environ. Heal. Res. 25:299-311. https://doi.org/10.1080/09603123.2014.938030
  30. Son, C.Y., Y.J. Jung, I.H. Lee, H.H. Kyoung, J.S. Lee and K.K. Kang. 2011. Studies on genetic variation of soluble solids, acidity and carotenoid contents in tomato fruits from germplasm. Korean J. Plant Res. 24:195-199 (in Korean). https://doi.org/10.7732/kjpr.2011.24.2.195
  31. Sun, T. and S.A. Tanumihardjo. 2007. An integrated approach to evaluate food antioxidant capacity. J. Food Sci. 72:R159-R165. https://doi.org/10.1111/j.1750-3841.2007.00552.x
  32. Tatiya, A.U., G.G. Tapadiya, S. Kotecha and S.J. Surana. 2011. Effect of solvents on total phenolics, antioxidant and antimicrobial properties of Bridelia retusa Spreng, stem bark. Indian J. Nat. Prod. Resour. 2:442-447.
  33. Taveira, M., F. Ferreres, A. Gil-Izquierdo, L. Oliveira, P. Valentao and P.B. Andrade. 2012. Fast determination of bioactive compounds from Lycopersicon esculentum Mill. leaves. Food Chem. 135:748-755. https://doi.org/10.1016/j.foodchem.2012.05.016
  34. Waterhouse, A.L. 2001. Determination of Total Phenolics: In Current Protocols in Food Analytical Chemistry. John Wiley & Sons, Inc., New York, U.S.A. pp. 11.1.1-11.1.7.
  35. Willcox, J.K., S.L. Ash and G.L. Catignani. 2004. Antioxidants and prevention of chronic disease. Crit. Rev. Food Sci. 44:275-295. https://doi.org/10.1080/10408690490468489
  36. Yang, S.R., Songzhyhao and H.O. Boo. 2015. Antioxidant activity of several cabbage (Brassica oleracea L.) cultivars. Korean J. Plant Res. 28:312-320. https://doi.org/10.7732/kjpr.2015.28.3.312
  37. Zhen, J., T.S. Villani, Y. Guo, Y.D. Qi, K. Chin, M.H. Pan, C.T. Ho, J.E. Simon and Q.L. Wu. 2016. Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves. Food Chem. 190:673-680. https://doi.org/10.1016/j.foodchem.2015.06.006