KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Use of Chlorophyll a Fluorescence Imaging for Photochemical Stress Assessment in Maize (Zea mays L.) Leaf under Hot Air Condition

  • Park, Jong Yong (Biological Research Part, Hanearl Science) ;
  • Yoo, Sung Young (Institute of Ecological Phytochemistry, Hankyong National University) ;
  • Kang, Hong Gyu (Department of Plant Life and Environmental Science, Hankyong National University) ;
  • Kim, Tae Wan (Department of Plant Life and Environmental Science, Hankyong National University)
  • Received : 2016.10.10
  • Accepted : 2016.11.18
  • Published : 2016.12.31
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Abstract

The objective of this study was to find a rapid determination of the hot air stress in maize (Zea mays L.) leaves using a portable chlorophyll fluorescence imaging instrument. To assess the photosynthetic activity of maize leaves, an imaging analysis of the photochemical responses of maize was performed with chlorophyll fluorescence camera. The observed chlorophyll imaging photos were numerically transformed to the photochemical parameters on the basis of chlorophyll a fluorescence. Chlorophyll a fluorescence imaging (CFI) method showed that a rapid decrease in maximum fluorescence intensity ($F_m$) of leaf occurred under hot air stress. Although no change was observed in the maximum quantum yield ($F_v/F_m$) of the hot air stressed maize leaves, the other photochemical parameters such as maximum fluorescence intensity ($F_m$) and Maximum fluorescence value ($F_p$) were relatively lowered after hot air stress. In hot air stressed maize leaves, an increase was observed in the nonphotoquenching (NPQ) and decrease in the effective quantum yield of photochemical energy conversion in photosystem II (${\Phi}PSII$). Thus, NPQ and ${\Phi}PSII$ were available to be determined non-destructively in maize leaves under hot air stress. Our results clearly indicated that the hot air could be a source of stress in maize leaves. Thus, the CFI analysis along with its related parameters can be used as a rapid indicating technique for the determining hot air stress in plants.

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References

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