DOI QR코드

DOI QR Code

산양유 발효유의 면역 활성 및 체력증진 효과

Effects of Fermented Goat Milk on Immunomodulatory Activity and Physical Strength in Mice

  • 오광훈 (공주대학교 사범대학 체육교육과) ;
  • 김아롱 (건양대학교 의과대학 미생물학교실) ;
  • 배종환 ((주)바이오프로젠) ;
  • 이경복 (건양대학교 의과대학 생화학교실) ;
  • 유영춘 (건양대학교 의과대학 미생물학교실)
  • Oh, Kwang-Hoon (Department of Physical Education, College of Education, Kongju National University) ;
  • Kim, A Rong (Department of Microbiology, College of Medicine, Konyang University) ;
  • Bae, Jong-Hwan (Research Institute, Bioprogen Co., Ltd.) ;
  • Lee, Kyung Bok (Department of Biochemistry, College of Medicine, Konyang University) ;
  • Yoo, Yung Choon (Department of Microbiology, College of Medicine, Konyang University)
  • 투고 : 2015.10.20
  • 심사 : 2015.12.09
  • 발행 : 2016.02.29

초록

본 연구에서는 산양유 발효유(F-GM)의 급여가 면역 활성과 체력증진에 미치는 영향을 조사하였다. F-GM을 경구 투여한 마우스의 비장세포는 림프구 유사분열 촉진인자인 ConA와 LPS에 대한 증식반응의 상승을 나타내었다. ConA를 처리한 비장세포로부터 분비되는 cytokine을 ELISA법에 의해 정량한 결과 F-GM 투여에 의해 IL-2와 IFN-${\gamma}$의 생성은 유의하게 증가하였으나, IL-4 및 IL-10의 생성은 별다른 변화를 보이지 않았다. 또한 RT-PCR을 이용한 이들 cytokine mRNA의 정량실험에서도 F-GM 투여로 IL-2와 IFN-${\gamma}$의 mRNA가 증가하는 것을 확인하였다. 한편 KLH(20 mg/mouse)를 정상 대조군 마우스 혹은 F-GM을 급이한 마우스에 각각 면역하고 5주째에 KLH에 대한 항체가를 측정한 결과 F-GM을 급이한 마우스에서 항체가가 유의하게 상승하는 것으로 나타났다. KLH에 대한 항체의 isotype을 조사한 실험에서는 KLH에 대한 IgG1, IgG2a 그리고 IgM 특이항체의 상승이 관찰되었다. F-GM 경구 투여가 세포성 면역에 미치는 영향을 측정하기 위하여 면역개시 7주째의 마우스를 이용하여 KLH에 대한 지연형 과민반응(DTH)을 측정하였다. 그 결과 F-GM을 경구 투여한 마우스에서 DTH의 유의한 상승효과가 확인되었다. 한편 수영시험을 통해 F-GM의 경구 투여가 체력증강에 미치는 영향을 측정한 결과 F-GM을 투여한 마우스에서 매우 유의한 수영시간의 연장 효과가 관찰되었다. 이들 결과로부터 F-GM은 면역세포의 활성화를 통한 면역력 강화는 물론 체력을 증가시키는 생리활성을 갖는 것으로 확인되었다.

In this study, the effects of fermented goat milk (F-GM) on immunological activity and physical strength were examined. Splenocytes obtained from mice administered with F-GM showed increased responsinveness to mitogens, concanavalin-A (ConA), and lipopolysaccharide (LPS). Treatment with F-GM also significantly augmented production of interleukin (IL)-2 and interferon (IFN)-${\gamma}$, but not IL-4 or IL-10 from ConA-stimulated splenocytes. The activity of F-GM administration to enhance production of IL-2 and IFN-${\gamma}$ was confirmed based on mRNA expression of these cytokines by reverse transcription polymerase chain reaction. After immunization with keyhole limpet hemocyanin (KLH, 20 mg/mouse), mice administered F-GM showed significantly higher antibody titers against KLH than those of phosphate-buffered saline-treated mice, and showed the highest titer 5 weeks after KLH immunization. Analysis for determining isotypes of antibodies revealed that administration of F-GM elicited KLH-specific antibody titers of IgG1, IgG2a, and IgM. In a delayed type hypersensitivity (DTH) test carried out 7 weeks after the primary immunization, F-GM-treated mice showed a higher DTH reaction than the control mice. Furthermore, the swimming test found that administration of F-GM significantly increased swimming time. These results suggest that administration of F-GM enhances not only immune responses against antigens but also physical strength.

키워드

참고문헌

  1. Park YW. 2000. Comparison of mineral and cholesterol composition of different commercial goat milk products manufactured in USA. Small Rumin Res 37: 115-124. https://doi.org/10.1016/S0921-4488(99)00144-3
  2. Bramanti E, Sortino C, Onor M, Beni F, Raspi G. 2003. Separation and determination of denatured ${\alpha}_{s1}-$, ${\alpha}_{s2}-$, ${\beta}-$ and ${\kappa}$-caseins by hydrophobic interaction chromatography in cows', ewes' and goats' milk, milk mixtures and cheeses. J Chromatogr A 994: 59-74. https://doi.org/10.1016/S0021-9673(03)00483-7
  3. Jandal JM. 1996. Comparative aspects of goat and sheep milk. Small Rumin Res 22: 177-185. https://doi.org/10.1016/S0921-4488(96)00880-2
  4. Nam MS, Shimazaki K, Kumura H, Lee KK, Yu DY. 1999. Characterization of Korean native goat lactoferrin. Comp Biochem Physiol B Biochem Mol Biol 123: 201-208. https://doi.org/10.1016/S0305-0491(99)00060-7
  5. Toral PG, Chilliard Y, Rouel J, Leskinen H, Shingfield KJ, Bernard L. 2015. Comparison of the nutritional regulation of milk fat secretion and composition in cows and goats. J Dairy Sci 398: 7277-7297.
  6. Pierre A, Michel F, Zahoute L. 1999. Composition of casein micelles in relation to size in goat milks with A and null ${\alpha}_{s1}$-casein variants. Int Dairy J 9: 179-182. https://doi.org/10.1016/S0958-6946(99)00057-6
  7. Manzi P, Pizzoferrato L. 2013. Taurine in milk and yoghurt marketed in Italy. Int J Food Sci Nutr 64: 112-116. https://doi.org/10.3109/09637486.2012.704906
  8. Lee KB, Song KS, Moon EH, Lee J, Yoo YC. 2009. Adjuvant activity of Cudrania tricuspidata water extracts to enhance antigen specific humoral and cellular immune responses. J Korean Soc Appl Biol Chem 52: 234-240. https://doi.org/10.3839/jksabc.2009.042
  9. dela Pena IJI, de la Pena JB, Cheong JH. 2014. The ameliorating effect of Hoveniae Semen seu Fructus extract against ethanol-induced psychomotor alterations in rats. Yakhak Hoeji 58: 300-306.
  10. Chae BS. 2002. Effects of chlorpyrifos on the production of splenic Th cytokines. J Environ Toxicol 17: 325-332.
  11. Barlow JL, McKenzie AN. 2014. Type-2 innate lymphoid cells in human allergic disease. Curr Opin Allergy Clin Immunol 14: 397-403. https://doi.org/10.1097/ACI.0000000000000090
  12. Adema GJ. 2008. Dendritic cells from bench to bedside and back. Immunol Lett 122: 128-130.
  13. Abbas AK, Murphy KM, Sher A. 1996. Functional diversity of helper T lymphocytes. Nature 383: 787-793. https://doi.org/10.1038/383787a0
  14. Yoon TJ, Yoo YC, Kang TB, Song SK, Doo MS, Kim JB. 1999. Immunoadjuvant activity of Korean mistletoe extract (Viscum album coloratum) to enhance humoral and cellular immune response. Kor J Immunol 21: 63-79.
  15. Tamura M, Yoo YC, Yoshimatsu K, Yoshida R, Oka T, Ohkuma K, Arikawa J, Azuma I. 1995. Effects of muramyl dipeptide derivatives as adjuvants on the induction of antibody response to recombinant hepatitis B surface antigen. Vaccine 13: 77-82. https://doi.org/10.1016/0264-410X(95)80015-6
  16. Ebringer L, Ferencik M, Krajcovic J. 2008. Beneficial health effects of milk and fermented dairy products-review. Folia Microbiol 53: 378-394. https://doi.org/10.1007/s12223-008-0059-1
  17. Cross ML, Stevenson LM, Gill HS. 2001. Anti-allergy properties of fermented foods: an important immunoregulatory mechanism of lactic acid bacteria?. Int Immunopharmacol 1: 891-901. https://doi.org/10.1016/S1567-5769(01)00025-X
  18. Salva S, Nunez M, Villena J, Ramon A, Font G, Alvarez S. 2011. Development of a fermented goats' milk containing Lactobacillus rhamnosus: in vivo study of health benefits. J Sci Food Agric 91: 2355-2362. https://doi.org/10.1002/jsfa.4467

피인용 문헌

  1. 산양유의 산업적 이용 가치에 대한 연구 고찰 vol.34, pp.3, 2016, https://doi.org/10.22424/jmsb.2016.34.3.173