한국환경보건학회지 (Journal of Environmental Health Sciences)

  • 제42권1호
  • /
  • Pages.34-40
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  • 2016
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  • 1738-4087(pISSN)
  • /
  • 2233-8616(eISSN)
한국환경보건학회 (Korean Society of Environmental Health)
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제약회사 폐수처리장 방류수 중 항생제 내성 Enterobacteria에 관한 연구

Study on Antibiotic Resistant Enterobacteria in Pharmaceutical Effluent

  • 김재건 (신한대학교 에너지환경공학과) ;
  • 김영진 (동남보건대학교 환경보건과)
  • Kim, Jae-Gun (Department of Energy and Environment Engineering, Shinhan University) ;
  • Kim, Young Jin (Department of Environmental Health, Dongnam Health University)
  • 투고 : 2015.12.07
  • 심사 : 2016.02.15
  • 발행 : 2016.02.29
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초록

Objectives: This study aims to examine the concentration, diversity, and antibiotic characteristics of penicillin G resistant enterobacteria present in pharmaceutical effluent. Methods: Water sampling was performed from a pharmaceutical company in Gyeonggi-do Province, Korea in March 2015. Water samples were plated in triplicate on tryptic soy agar plates with 32 mg/L of penicillin G. Penicillin G resistant enterobacteria were selected from the effluent and were subjected to 16S rRNA analysis for penicillin G resistant species determination. Identified resistant strains were tested for resistance to various antibiotics. Results: Penicillin G resistant enterobacteria were present at 6.2% as to culturable heterotrophic bacteria. Identified penicillin G resistant enterobacteria exhibited resistance to more than 10 of the antibiotics studied. These resistant bacteria are gram negative and are closely related to pathogenic species. Conclusion: Multi-antibiotic resistant bacteria in the effluent suggest a need for disinfection and advanced oxidation processes for pharmaceutical effluents.

키워드

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