Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Study on Antibiotic Resistant Enterobacteria in Pharmaceutical Effluent

제약회사 폐수처리장 방류수 중 항생제 내성 Enterobacteria에 관한 연구

  • Kim, Jae-Gun (Department of Energy and Environment Engineering, Shinhan University) ;
  • Kim, Young Jin (Department of Environmental Health, Dongnam Health University)
  • 김재건 (신한대학교 에너지환경공학과) ;
  • 김영진 (동남보건대학교 환경보건과)
  • Received : 2015.12.07
  • Accepted : 2016.02.15
  • Published : 2016.02.29
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Abstract

Objectives: This study aims to examine the concentration, diversity, and antibiotic characteristics of penicillin G resistant enterobacteria present in pharmaceutical effluent. Methods: Water sampling was performed from a pharmaceutical company in Gyeonggi-do Province, Korea in March 2015. Water samples were plated in triplicate on tryptic soy agar plates with 32 mg/L of penicillin G. Penicillin G resistant enterobacteria were selected from the effluent and were subjected to 16S rRNA analysis for penicillin G resistant species determination. Identified resistant strains were tested for resistance to various antibiotics. Results: Penicillin G resistant enterobacteria were present at 6.2% as to culturable heterotrophic bacteria. Identified penicillin G resistant enterobacteria exhibited resistance to more than 10 of the antibiotics studied. These resistant bacteria are gram negative and are closely related to pathogenic species. Conclusion: Multi-antibiotic resistant bacteria in the effluent suggest a need for disinfection and advanced oxidation processes for pharmaceutical effluents.

Keywords

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