Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Antioxidant and Antiproliferative Activity of Extracts from Water Chestnut (Trapa japonica Flerow)

마름 추출물의 항산화활성 및 암세포증식 억제 활성

  • Han, Hye Min (Applied Plant Sciences Program, Kangwon National University) ;
  • Kwon, Yong Soo (Department of Pharmacy, Kangwon National University) ;
  • Kim, Myong Jo (Applied Plant Sciences Program, Kangwon National University)
  • 한혜민 (강원대학교 식물자원응용과학전공) ;
  • 권용수 (강원대학교 약학과) ;
  • 김명조 (강원대학교 식물자원응용과학전공)
  • Received : 2015.10.05
  • Accepted : 2015.12.03
  • Published : 2016.02.28
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Abstract

Background : This study aimed to investigate the antioxidant and antiproliferative activities of extract from different parts of water chestnut (Trapa japonica Flerow). Methods and Results : The total polyphenol content of pericarp and seed extract was 438.31 mg/g and 25.32 mg/g respectively. DPPH radical scavenging assay showed that the half maximal inhibitory concentration ($IC_{50}$ values) of pericarp and seed extract were $5.28{\mu}g/m{\ell}$ and $355.51{\mu}g/m{\ell}$ respectively. In addition, the pericarp extract showed strong reducing power. In the MTT assay, the pericarp extract significantly inhibited the viability of A549, AGS, HeLa, PC-3, HCT116, HT29 and SW620 cell lines compared with the seed extract. Conclusions : These results suggest that T. japonica Flerow extracts have significant antioxidant and antiproliferative activity.

Keywords

References

  1. Ahn MS, Kim HJ and Seo MS. (2007). A study on the antioxidative and antimicrobial activities of the Citrus Unshju peel extracts. Journal of the Korean Society of Dietary Culture. 22:454-461.
  2. An BJ, Lee JT, Kwak JH, Park JM, Lee JY, Son JH, Bae JH and Choe C. (2004). Biological activity of polyphenol group fraction from Korean pear peel. Journal of the Korean Society for Applied Biological Chemistry. 47:92-95.
  3. Anwar F, Shaheen N, Shabir G, Ashraf M, Alkharfy KM and Gilani AH. (2013). Variation in antioxidant activity and phenolic and flavonoid contents the flowers and leaves of Ghaneri(Lantana camara L.) as affected by different extraction solvents. International Journal of Pharmacology. 9:442-453. https://doi.org/10.3923/ijp.2013.442.453
  4. Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 181:1199-1200. https://doi.org/10.1038/1811199a0
  5. Bruggisser R, von Daeniken K, Jundt G, Schaffner W and Tullberg-Reinert H. (2002). Interference of plant extracts, phytoestrogens and antioxidants with the MTT tetrazolium assay. Planta medica. 68:445-448. https://doi.org/10.1055/s-2002-32073
  6. Choi JS, Kim JS, Lim HK, Kwak HH and Cho KY. (1997). Study on the seed germination characteristics of Trapa japonica FEROV. Korean Journal of Weed Science. 17:413-420.
  7. Chung IJ. (2003). Cancer gene therapy: History and major developments. Journal of Toxicology and Public Health. 19:247-257.
  8. Han KI, Kim MR, Jo BK, Kim MJ, Kang MJ, Park KH, Koo YE, Kim BS, Jung EG and Han MD. (2015). Antimicrobial and antioxidative activities of the extracts from walnut(Juglans regia L.) green husk. Journal of Life Science. 25:422-440.
  9. Hong JI, Kim HJ and Kim JY. (2011). Factors affecting reactivity of various phenolic compounds with the Folin-Ciocalteu reagent. Journal of Korean Society of Food Science and Nutrition. 40:205-213. https://doi.org/10.3746/jkfn.2011.40.2.205
  10. Hyun MR, Lee YS and Park YH. (2011). Antioxidative activity and flavonoid content of Chrysanthemum zawadskii flowers. Korean Journal of Horticultural Science and Technology. 29:68-73.
  11. Im DY and Lee KI. (2013). Antioxidative activity and tyrosinase inhibition effect of ethanol extract and its fractions from the branch of Rhododendron schlippenbachii. Korean Journal of Medicinal Crop Science. 21:439-443. https://doi.org/10.7783/KJMCS.2013.21.6.439
  12. Jeong HR, Choi GN, Kim JH, Kwak JH, Kim YS, Jeong CH, Kim DO and Heo HJ. (2010). Nutritional components and their antioxidative protection of neuronal cells of litchi(Litchi chinensis Sonn.) fruit pericarp. Korean Journal of Food Science and Technology. 42:481-487.
  13. Kang MH, Ryu SN, Min KS, Kim KS, Bang JK and Lee BH. (1999). Current research activities of antioxidative compound in plants. Korean Journal of International Agriculture. 11:207-215.
  14. Kang MJ, Lee SK, Song JH, Kim ME, Kim MJ, Jang JS, Lee JH and Kim JI. (2009). Water chestnut(Trapa japonica Flerov.) exerts inhibitory effect on postprandial glycemic response in rats and free radical scavenging activity in vitro. Food Science and Biotechnology. 18:808-812.
  15. Kim DH. (2014). Recognition of cancer risk and consumption of cancer insurance possibility of adverse selection. Journal of Insurance Studies. 25:35-62.
  16. Kim EJ, Choi JY, Yu MR, Kim MY, Lee SH and Lee BH. (2012). Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Korean Journal of Food Science and Technology. 44:337-342. https://doi.org/10.9721/KJFST.2012.44.3.337
  17. Kim JN, Chae H, Kwon YK and Kim BJ. (2014a). Effects of Schisandra chinensis on human breast cancer cells. Korean Journal of Oriental Physiology and Pathology. 28:162-168.
  18. Kim JS, Lee YJ, Yang JF, Sa YJ, Kim MO, Park JH, Park DS, Yu CY and Kim MJ. (2013). Biological activity of Sorghum bicolor M. cv. Bulgeunjangmoksusu extracts. Korean Journal of Plant Resources. 26:111-118. https://doi.org/10.7732/kjpr.2013.26.1.111
  19. Kim KH, Park HM, O SH and Lee CH. (1995). Tetrazolium (MTT) assay for chemosensitivity testing of the squamous cell carcinoma cell lines of the head and neck. Korean Journal of Otolaryngology-Head and Neck Surgery. 38:1225-1236.
  20. Kim YS, Hwang JW, Han YK, Kwon HJ, Hong H, Kim EH, Moon SH, Jeon BT and Park PJ. (2014b). Antioxidant activity and protective effects of Trapa japonica pericarp extracts against tert-butylhydroperoxide-induced oxidative damage in Chang cells. Food and Chemical Toxicology. 64:49-56. https://doi.org/10.1016/j.fct.2013.11.018
  21. Lee SK. (2008). Anti-diabetic effect of Trapa japonica Flerov. Master Thesis. Inje University. Korea. 1-70.
  22. Liu RH. (2004). Potential synergy of phytochemicals in cancer prevention: Mechanism of action. Journal of Nutrition. 134 (Supplement 12):3479S-3485S. https://doi.org/10.1093/jn/134.12.3479S
  23. Lue BM, Nielsen NS, Jacobsen C, Hellgren L, Guo Z and Xu X. (2010). Antioxidant properties of modified rutin esters by DPPH, reducing power, iron chelation and human low density lipoprotein assays. Food Chemistry. 123:221-230. https://doi.org/10.1016/j.foodchem.2010.04.009
  24. Lugemwa FN, Snyder AL and Shaikh K. (2013). Determination of radical scavenging activity and total phenols of wine and spices: A randomized study. Antioxidants. 2:110-121. https://doi.org/10.3390/antiox2030110
  25. Moon JM, Seok GH and Cho SI. (2012). Antiproliferative effect of Schisandrae fructus extract on PC-3 human prostate cancer cells. The Korea Journal of Herbology. 27:17-23.
  26. Moreno MIN, Isla MI, Sampietro AR and Vattuone MA. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  27. Mosmann T. (1983). Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 65:55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  28. Nam JJ, Lee KE, Park JE, Moon SJ and Youm JK. (2014). Inhibitory effect of fractionated Trapa japonica extracts on UVB-induced skin photoaging. Journal of the Society of Cosmetic Scientists of Korea. 40:321-330. https://doi.org/10.15230/SCSK.2014.40.4.321
  29. Oyaizu M. (1986). Studies on products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics. 44:307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  30. Park HS. (2011). Antioxidant activity of solvent extracts from Vitis coignetiae skins. The Korean Journal of Culinary Research. 17:208-217.
  31. Park SJ and Lee HY. (2015). Component analysis and antioxidant activity of Wasabi japonica Matsum leaves. Korean Journal of Medicinal Crop Science. 23:207-213. https://doi.org/10.7783/KJMCS.2015.23.3.207
  32. Park YO and Lim HS. (2009). Antioxidant activities of bamboo(Sasa Borealis) leaf extract according to extraction solvent. Journal of Korean Society Food Science and Nutrition. 38:1640-1648. https://doi.org/10.3746/jkfn.2009.38.12.1640
  33. Sharma OP and Bhat TK. (2009). DPPH antioxidant assay revisited. Food Chemistry. 113:1202-1205. https://doi.org/10.1016/j.foodchem.2008.08.008
  34. Stankovic MS, Niciforovic N, Mihailovic V, Topuzovic M and Solujic S. (2012). Antioxidant activity, total phenolic content and flavonoid concentrations of different palnt parts of Teucrium polium L. subsp. poloum. Acta Societatis Botanicorum Poloniae. 81:117-122. https://doi.org/10.5586/asbp.2012.010
  35. Suh JT, Choi EY, Yoo DL, Kim KD, Lee JN, Hong SY, Kim SJ, Nam JH, Han HM and Kim MJ. (2015). Comparative study of biological activities at different harvesting times and new varieties for highland culture of Gom-chwi. Korean Journal of Plant Resource. 28:391-399. https://doi.org/10.7732/kjpr.2015.28.4.391
  36. Taga MS, Miller EE and Pratt DE. (1984). Chia seeds as a source of natural lipid antioxidants. Journal of the American Oil Chemists' Society. 61:928-931. https://doi.org/10.1007/BF02542169