Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibitory Effects of the EtOH Extract of Aster koraiensis on AGEs formation in STZ-induced diabetic rats and AGEs-induced Protein Cross-linking in vitro

벌개미취 에탄올추출물의 STZ-유도 당뇨 모델에서의 최종당화산물의 생성 및 교차결합에 미치는 효과

  • Kim, Junghyun (Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Chan-Sik (Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Jin Sook (Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine)
  • 김정현 (한국한의학연구원 한의약융합연구부) ;
  • 김찬식 (한국한의학연구원 한의약융합연구부) ;
  • 김진숙 (한국한의학연구원 한의약융합연구부)
  • Received : 2016.08.11
  • Accepted : 2016.10.13
  • Published : 2016.12.30
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Abstract

Advanced glycation end products (AGEs) such as $N^{\varepsilon}$-(carboxy-methyl)lysine (CML) have been implicated in the development of diabetic nephropathy. The aim of this study was to investigate the inhibitory effects of ethanolic extract of Aster koraiensis (AKE) on AGEs formation and AGEs-collagen cross-linking in vitro and CMLs formation in streptozotocin (STZ)-induced diabetic rats. AKE significantly inhibited AGEs formation ($IC_{50}$ value of $18.74{\mu}g/mL$) and AGEs-collagen cross-linking ($IC_{50}$ value of 0.274 mg/mL) in vitro than the well-known glycation inhibitor aminoguanidine ($IC_{50}$ value of $72.12{\mu}g/mL$ and 1.99 mg/mL, respectively). AKE (100 mg/kg per day) was given to diabetic rats for 9 weeks. In STZ-induced diabetic rats, severe hyperglycemia was developed, and urinary CMLs and plasma CMLs were markedly increased. Immunohistochemical stain revealed that CMLs were accumulated within renal glomerulus in STZ-induced diabetic rats. However, AKE significantly reduced urinary CMLs and plasma CMLs in diabetic rats. CMLs accumulation was inhibited by AKE treatment in the renal glomerulus. These results suggest that AKE had an inhibitory effect of AGE accumulation in the glomeruli of diabetic rat and could be an inhibitor of AGE-induced protein cross-linking. The oral administration of AKE may significantly help to prevent the progression of diabetic nephropathy in patients with diabetes.

Keywords

References

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