한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제44권4호
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  • Pages.442-451
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  • 2016
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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NF-κB와 MAPKs 활성 저해를 통한 미야베 모자반(Sargassum miyabei Yendo) 에탄올 추출물의 항염증 활성

Anti-Inflammatory Activity of Ethanol Extract of Sargassum miyabei Yendo via Inhibition of NF-κB and MAPK Activation

  • 김민지 (부경대학교 식품공학과/식품연구소) ;
  • 배난영 (부경대학교 식품공학과/식품연구소) ;
  • 김꽃봉우리 (부경대학교 수산과학연구소) ;
  • 박선희 (부경대학교 식품공학과/식품연구소) ;
  • 장미란 (식품의약품안전처 건강기능식품정책과) ;
  • 임무혁 (대구대학교 식품공학과) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Kim, Min-Ji (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Bae, Nan-Young (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University) ;
  • Park, Sun-Hee (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Jang, Mi-Ran (Health Functional Food Policy Division, Ministry of Food and Drug Safety) ;
  • Im, Moo-Hyeog (Department of Food Science and Biotechnology, Daegu University) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
  • 투고 : 2016.07.04
  • 심사 : 2016.10.24
  • 발행 : 2016.12.28
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초록

본 연구에서는 미야베 에탄올 추출물의 항염증 활성을 확인하기 위해 LPS로 활성화된 RAW 264.7 세포와 croton-oil로 유도된 귀부종 동물 모델을 이용하였다. 그 결과, SMYEE 50 및 $100{\mu}g/ml$ 농도처리 시, LPS로 유도된 염증반응에서 $NF-{\kappa}B$ 활성 억제와 더불어 MAPKs의 인산화를 효과적으로 억제함을 보였다. LPS에 의해 증가된 NO와 전염증성 사이토카인의 분비량도 농도 의존적으로 감소하였다. 또한 SMYEE는 croton oil로 부종을 유발한 마우스모델에서 귀부종 억제효과를 나타내었고, 조직의 진피 두께 및 mast cell의 수가 현저히 감소하였음을 확인하였다. 이를 통해 SMYEE는 염증 반응의 전사인자인 $NF-{\kappa}B$ 및 MAPKs의 활성을 조절함으로써 iNOS와 COX-2의 발현 및 전염증성 매개인자인 NO, IL-6, $TNF-{\alpha}$ 및 $IL-1{\beta}$의 분비를 억제하여 항염증 활성을 가지는 것을 확인하였다. 현재까지 미야베 모자반내의 항염증 효능 물질에 관한 연구는 보고되지 않고 있으며 향후 실험을 통해 미야베 모자반 에탄올 추출물로부터 항염증 효과를 가지는 유효성분을 밝히기 위한 분리 연구를 진행할 예정이다.

The aim of this study was to investigate the anti-inflammatory effect of Sargassum miyabei Yendo ethanol extract (SMYEE) using RAW 264.7 cells and croton oil-induced Balb/c mice. SMYEE inhibited the production of pro-inflammatory cytokines [interleukin (IL)-6, tumor necrosis factor $(TNF)-{\alpha}$, and $IL-1{\beta}$] and nitric oxide in lipopolysaccharide (LPS)-induced inflammatory response. In addition, SMYEE suppressed the expression of inducible nitric oxide, cyclooxygenase-2, and nuclear factor-kappa B. Further, SMYEE inhibited the expression of mitogen-activated protein kinases (MAPKs), such as extra cellular signal-regulated kinase 1/2, p38, and c-Jun N-terminal kinase. In ear edema test, edema formation in the SMYEE treatment was lower than that in the positive control and was similar to that in the prednisolone treatment group. Photomicrographs of mice ear tissue showed a reduction in dermal thickness and number of infiltrated mast cells. Therefore, our results indicate that SMYEE exerts an anti-inflammatory effect via inhibition of nuclear factor ${NF}-{\kappa}B$ and MAPK activation and can be used as a natural source of anti-inflammatory compounds.

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