농약과학회지 (The Korean Journal of Pesticide Science)

  • 제20권4호
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  • Pages.349-354
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  • 2016
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
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살균제 디페노코나졸 분해 세균 분리 및 특성 분석

Isolation and Characterization of Soil Bacteria Degrading a Fungicide Defenoconazole

  • 안재형 (국립농업과학원 농업미생물과) ;
  • 노유미 (국립농업과학원 농업미생물과) ;
  • 이관형 (국립농업과학원 농업미생물과) ;
  • 박인철 (국립농업과학원 농업미생물과) ;
  • 김완규 (국립농업과학원 농업미생물과) ;
  • 한병학 (국립농업과학원 농업미생물과) ;
  • 유재홍 (국립농업과학원 농업미생물과)
  • Ahn, Jae-Hyung (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Ro, Yu-Mi (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Lee, Gwan-Hyeong (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Park, InCheol (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Kim, Wan-Gyu (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Han, Byeong-Hak (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • You, Jaehong (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA))
  • 투고 : 2016.10.22
  • 심사 : 2016.11.29
  • 발행 : 2016.12.31
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초록

트리아졸계 살균제는 세계적으로 사용량이 가장 많은 살균제 중 하나로 토양 반감기가 상대적으로 길다. 본 연구에서는 전국 과수원 토양으로부터 트리아졸계 살균제 테부코나졸, 플루퀸코나졸, 디페노코나졸을 분해할 수 있는 세균의 순수 분리를 시도하였다. 농화배양 과정에서 세 종의 살균제 중 디페노코나졸만이 100% 분해되었으며 이 배양액에서 디페노코나졸을 분해하는 세균 균주들을 순수분리하였다. rep-PCR 밴드 패턴 비교를 통해 이 균주들은 모두 동일 균주임을 확인하였으며 이 중 C8-2 균주만을 이후 연구에 사용하였다. 이 균주는 16S rRNA 유전자 염기서열에 기반하여 Sphingomonas 속 C8-2 균주로 동정되었으며 최소배지에서 100 mg/L의 디페노코나졸을 24시간 내에 분자량 296의 물질로 전환하였다. 이 분해산물은 토양 세균 및 곰팡이에 대한 저해 효과가 디페노코나졸에 비해 현저히 감소하여 향후 토양 및 농작물의 디페노코나졸 무독화에 C8-2 균주를 사용할 수 있을 것으로 기대한다.

Triazole fungicides occupy an important portion in the global fungicide market and are relatively persistent in soil compared to the other fungicides, suggesting possible adverse effects of the fungicides on human health and environment. In this study, we tried to isolate microorganisms from orchard soils, which can decompose the triazole fungicides, tebuconazole, fluquinconazole, and difenoconazole. Only difenoconazole was completely degraded in the enrichment culture, from which several difenoconazole-degrading bacteria were isolated. They showed the same rep-PCR pattern thus only one strain, C8-2, was further studied. The strain was identified as Sphingomonas sp. C8-2 based on its 16S rRNA gene sequence and decomposed 100 mg/L of difenoconazole in a minimum medium to an unknown metabolite with a molecular weight of 296 within 24 hours. The inhibition effect of the metabolite against representative soil microorganisms significantly decreased compared to that of difenoconazole thus the bacterial strain is expected to be used for the detoxification of difenoconazole in soil and crop.

키워드

참고문헌

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피인용 문헌

  1. Isolation and Characterization of Rhodococcus sp. Strains Capable of Degrading Benzimidazole Fungicides Benomyl and Carbendazim vol.24, pp.2, 2016, https://doi.org/10.7585/kjps.2020.24.2.163