한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)

  • 제29권4호
  • /
  • Pages.24-33
  • /
  • 2016
  • /
  • 1738-6640(pISSN)
  • /
  • 2234-4020(eISSN)
대한한방안이비인후피부과학회 (The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology)
권호 표지
DOI QR코드

DOI QR Code

통비산(通痺散) 열수추출물의 항염증반응 및 항산화활성에 대한 연구

Anti-Inflammatory Effects of Tongbi-san(通痺散) Extract on RAW264.7 Macrophages

  • 김용민 (세명대학교 한방바이오융합과학부) ;
  • 김희택 (세명대학교 한의과대학 한방안이비인후피부과학과) ;
  • 김이화 (세명대학교 한의과대학 경락경혈학교실)
  • Kim, Yong-Min (School of Oriental Medicine and Bio Convergence Sciences, Semyung University) ;
  • Kim, Hee-Taek (Dept. of Korean Medical Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Ee-Hwa (Dept. of Meridian & Acupoint, College of Korean Medicine, Semyung University)
  • 투고 : 2016.09.26
  • 심사 : 2016.11.10
  • 발행 : 2016.11.25
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : This study is to investigate the anti-inflammatory and anti-oxidant effects of Tongbi-san extract (TS) on RAW264.7 macrophages using by cell cytotoxicity, Nitric Oxide (NO) and Prostaglandin $E_2$ ($PGE_2$) production and 1,1-diphenyl-2-picryl ghdrazyl (DPPH) free radical scavenging capability. Methods : Cell cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The production of NO was measured by Griess assay. The production of $PGE_2$ was measured by immunoassay. And the anti-oxidant activity was measured by the DPPH method. Results : TS did not increased significantly compared to the TS untreated group in the cell cytotoxicity. TS inhibited NO and $PGE_2$ production in lipopolysaccharide-stimulated RAW 264.7 cells. TS had the DPPH free radical scavenging capability. Conclusion : The anti-inflammtory and anti-oxidant effects of TS may be use for a treatment of anti-inflammatory diseases.

키워드

참고문헌

  1. Cordell GA. Phytochemistry and traditional medicine-the revolution continues. Phytochem Lett. 2014;10:28-40. https://doi.org/10.1016/j.phytol.2014.07.003
  2. World Health Organization. WHO Traditional medicine strategy. 2013. Availble from:URL: http://www.who.int/medicines/publications/traditional/trm-strategy14_23.
  3. Torres-Rodrigues ML, Garcia-Chavez E, Berhow M, de Mejia EG. Anti-inflammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages. J Ethnopharmacol. 2016;188:266-74. https://doi.org/10.1016/j.jep.2016.04.057
  4. Tracey KJ. The inflammatory reflex. Nature. 2002;420:853-9. https://doi.org/10.1038/nature01321
  5. Chen X, Miao J, Wang H, Zhao F, Hu J, Gao P, et al. The anti-inflammatory activities of Ainsliaea fragrans Champ. extract and its components in lipopolysaccharide-stimulated RAW264.7 macrophages through inhibition of NF-${\kappa}B$ pathway. J Ethnopharmacol. 2015;170:72-80. https://doi.org/10.1016/j.jep.2015.05.004
  6. Devasagayam TPA, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. Journal of the Association of Physicians of India. 2004;52:794-804.
  7. Nakagawa T, Yokozawa T. Direct scavenging of nitric oxide and superoxide by green tea. Food Chem. Toxicol. 2002;40:1745-50. https://doi.org/10.1016/S0278-6915(02)00169-2
  8. Kim HK, Cheon BS, Kim YH, Kim SY, Kim HP. Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and their structure-activity relationships. Biochem. Pharmacol. 1999;58:759-65. https://doi.org/10.1016/S0006-2952(99)00160-4
  9. Surh YJ, Chun KS, Cha HH, Han SS, Keum Y, Park K, et al. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: downregulation of COX-2 and iNOs through suppression of $NF{\kappa}B$ activation. Mutat Res. 2001;480-481:243-68. https://doi.org/10.1016/S0027-5107(01)00183-X
  10. Chang YC, Li PC, Chen BC, Chang MS, Wang JL, Chiu WT, et al. Lipoteichoic acid-induced nitric oxide synthase expression in RAW 264.7 macrophages is mediated by cyclooxygenase-2, prostaglandin $E_2$, protein kinase A, p38 MAPK, and nuclear factor-kappa B pathways. Cell Signal. 2006;18:1235-43. https://doi.org/10.1016/j.cellsig.2005.10.005
  11. Zhang L. Zhang's Treatise on general medicine. 2nd ed. Beijing:Traditional Chinese Medical Publishing Company in China. 1995;809-10.
  12. Lim HH, Kim YO, Seo MJ, Choi SW. Anti-Inflammatory Effects of Volatile Flavor Extract from Herbal Medicinal Prescriptions Including Cnidium officinale Makino and Angelica gigas Nakai. Journal of the society of cosmetics scientists of korea. 2011;37:199-210.
  13. Han KS, Kim KC, Wang JH, Kim HJ. Effect of Unfermented and Fermented Atractylodes macrocephalae on Gut Permeability and Lipopolysaccharide-Induced Inflammation. Journal of Society of Korean Medicine for Obesity Research. 2013;13:24-32.
  14. Cho JH, Kwon JE, Cho Y, Kim I, Kang SC. Anti-inflammatory effect of Angelica gigas via Heme Oxygenase-1 expression. Nutrients. 2015;15:4862-74.
  15. Chen LG, Jan YS, Tsai PW, Norimoto H, Michihara S, Murayama C, et al. Antiinflammatory and Antinociceptive constituents of Atractylodes joponica koidzumi. J Agric Food Chem. 2016;64:2254-62. https://doi.org/10.1021/acs.jafc.5b05841
  16. Xu L, Kitade H, Ni Y, Ota T. Roles of chemokines and chemokine receptors in obesity-associated insulin resistance and nonalcoholic fatty liver disease. Biomolecules. 2015;5:1563-79. https://doi.org/10.3390/biom5031563
  17. Tilg H, Moschen AR. Inflammatory mechanisms in the regulation of insulin resistance. Mol Med. 2008;14:222-31.
  18. Hotamisligil GS. Inflammation and metabolic disorders. Nature. 2006;444:860-7. https://doi.org/10.1038/nature05485
  19. Reed GA, Arneson DW, Putnam WC, Smith HJ, Gray JC, Sullivan DK, et al. Single-dose and multiple-dose administration of indole-3-carbinol to women: pharmacokinetics based on 3,3'-diindolymethane. Cancer Epidermiol. Biomarkers Prev. 2006;15:2477-81. https://doi.org/10.1158/1055-9965.EPI-06-0396
  20. Xia Z, Triffitt JT. A review on macrophage response to biomaterials. Biomed. Mater. 2006;1:1-9. https://doi.org/10.1088/1748-6041/1/1/001
  21. Ross JA, Auger MJ, Burke B, Lewis CE. The biology of the macrophage. In:Burker B, Lewis CE(Eds.). The Macrophage. 2nd ed. Oxford:Oxford Medical Publications. 2002:1-72.
  22. Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, et al. Defective LPS signaling in C3H/Hej and C57BL/10ScCr mice. Mutations in Tlr4 gene. Science. 1998;282;2085-8. https://doi.org/10.1126/science.282.5396.2085
  23. Schmidt HH, Walter U. NO at work. Cell. 1994;78:919-25. https://doi.org/10.1016/0092-8674(94)90267-4
  24. St Clair EW, Wilkinson WE, Lang T, Sanders L, Misukonis MA, Gilkeson GS, et al. Increased expression of blood mononuclear cell nitric oxide synthase type 2 in rheumatoid arthritis patients. Journal of Experimental Medicine. 1996;184:1173-8. https://doi.org/10.1084/jem.184.3.1173
  25. Worrall NK, Lazenby WD, Misko TP, Lin TS, Rodi CP, Manning PT, et al. Modulation of in vivo alloreactivity by inhibition of inducible nitric oxide synthase. Journal of Experimental Medicine. 1995;181:63-70. https://doi.org/10.1084/jem.181.1.63
  26. Chiou WF, Chou CJ, Chen CF. Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages. Life Sciences. 2001;69:625-35. https://doi.org/10.1016/S0024-3205(01)01154-7
  27. Hu XD, Yang Y, Zhong XG, Zhang XH, Zhang YN, Zheng ZP, et al. Antiinflammatory effects of Z23 on LPS-induced inflammatory responses in RAW264.7 macrophages. Journal of Ethnopharmacology. 2008;120:447-51. https://doi.org/10.1016/j.jep.2008.09.026
  28. Bak MJ, Truong BL, Kang HS, Jun M, Jeong WS. Anti-inflammatory effect of procyanidins from wild grape (Vitis amurensis) seeds in LPS-induced RAW264.7 cells. Oxid Med Cell Longev. 2013:36;340-8.
  29. Parthasarathy S, Khan-Marchant N, Penumeteha M, Santanam N. Oxidative stress in cardiovascular disease. J Nuclear Cardiol. 2001;8(3):379-89. https://doi.org/10.1067/mnc.2001.114150
  30. Benedetti S, Benvenah F, Pagharami S, Francegh S, Stephano S, Canestrari F. Antioxidant properties of a novel phycocyania extract from the blue-green alga Aphanizomenonflos-aquae. Life sci. 2004;75:2353-62. https://doi.org/10.1016/j.lfs.2004.06.004
  31. Guerci B, Bohme P, Kearney-Schwartz A, Zannad F, Drouin P. Endothelial dysfunction and type 2 diabetes. Diabetes Metab. 2001;27:436-47.
  32. Pincemail J, Meurisse M, Limet R, Defraigne JO. L'evaluation du stress oxydatif d'un individu : une realite pour le medecin. Vaisseaux Coeur Poumons. 1999;4(5):12-23.
  33. Drobek-Slowik M, Karezewicz DK. The potential role of oxidative stress in the pathogenesis of the age-related macular degeneration (AMD). Postepy Hig Med Dosw. 2007;61:28-37.
  34. Zafra-Stones S, Yasmin T, Bagchi M, Chattejee A, Vinson JA, Bagchi D. Berry anthocyanins as novel antioxidants in human health and disease prvention. Mol Nutr Food Res. 2007;51:675-83. https://doi.org/10.1002/mnfr.200700002
  35. Tang Q, Chen L, Jie Y. Effects of traditional Chinese medicine Drynaria fortuneismith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells. China Journal of Chinese Mareria Medica. 2004;29(2):64-168.