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Anti-Inflammatory Effects of Tongbi-san(通痺散) Extract on RAW264.7 Macrophages

통비산(通痺散) 열수추출물의 항염증반응 및 항산화활성에 대한 연구

  • Kim, Yong-Min (School of Oriental Medicine and Bio Convergence Sciences, Semyung University) ;
  • Kim, Hee-Taek (Dept. of Korean Medical Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Ee-Hwa (Dept. of Meridian & Acupoint, College of Korean Medicine, Semyung University)
  • 김용민 (세명대학교 한방바이오융합과학부) ;
  • 김희택 (세명대학교 한의과대학 한방안이비인후피부과학과) ;
  • 김이화 (세명대학교 한의과대학 경락경혈학교실)
  • Received : 2016.09.26
  • Accepted : 2016.11.10
  • Published : 2016.11.25

Abstract

Objectives : This study is to investigate the anti-inflammatory and anti-oxidant effects of Tongbi-san extract (TS) on RAW264.7 macrophages using by cell cytotoxicity, Nitric Oxide (NO) and Prostaglandin $E_2$ ($PGE_2$) production and 1,1-diphenyl-2-picryl ghdrazyl (DPPH) free radical scavenging capability. Methods : Cell cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The production of NO was measured by Griess assay. The production of $PGE_2$ was measured by immunoassay. And the anti-oxidant activity was measured by the DPPH method. Results : TS did not increased significantly compared to the TS untreated group in the cell cytotoxicity. TS inhibited NO and $PGE_2$ production in lipopolysaccharide-stimulated RAW 264.7 cells. TS had the DPPH free radical scavenging capability. Conclusion : The anti-inflammtory and anti-oxidant effects of TS may be use for a treatment of anti-inflammatory diseases.

Keywords

References

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