Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Anti-oxidant, Anti-coagulation, and Anti-platelet Aggregation Activities of Black Currant (Ribes nigrum L.)

블랙커런트의 항산화, 항응고 및 혈소판 응집저해 활성

  • Kim, Mi-Sun (Dept. of Food and Nutrition, Andong National University) ;
  • Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
  • Received : 2016.07.26
  • Accepted : 2016.09.26
  • Published : 2016.12.30
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Abstract

The black currant (Ribes nigrum L.) is belong to the Grossulariaceae family, and has piquant berries, which can be eaten as raw or as processed foods, such as jams, jelly, juice and syrups. In this study, the fresh juice of black currant (FJBC) from Austria and its subsequent organic solvent fractions, such as hexane fraction, ethylacetate (EA) fraction, butanol fraction and water residue, were prepared and their in-vitro anti-oxidant, anti-coagulation and anti-platelet aggregation activities were evaluated. The FJBC and EA fraction, which has concentrated polyphenol and flavonoid, showed strong radical scavenging activities and reducing power. The $RC_{50}s$ of EA fraction against DPPH anion, ABTS cation, nitrite were 136.3, 66.2 and $115.5{\mu}g/ml$, respectively, those are 1/10, 1/16, and 1/7.7 of $RC_{50}s$ of vitamin C. In anti-coagulation assay, the FJBC, EA and butanol fraction showed significant inhibitory activities against thrombin, prothrombin and coagulation factors. Furthermore, the anti-platelet aggregation activities of EA and butanol fraction were the stronger than that of aspirin. The concentrations required for 50% platelet aggregation inhibition of aspirin, EA and butanol fraction were 0.395, 0.192 and 0.261 mg/ml, respectively. The EA and butanol fraction have no hemolysis activities up to 0.5 mg/ml against human red blood cells. The results suggest that the FJBC and its EA and butanol fraction have high potentials as novel anti-thrombosis agents. This report provides the first evidence of anti-thrombosis activity of black currant.

블랙커런트는 장미목 범의귀과의 낙엽관목으로, 즙이 많은 검은 열매와 잎을 주스, 잼, 젤리, 시럽 등으로 가공하여 식용하고 있다. 본 연구에서는 블랙커런트(오스트리아산)의 착즙액과 이의 순차적 유기용매 분획물인 hexane 분획물, ethylacetate (EA) 분획물, butanol 분획물 및 물 잔류물을 조제하여 각각의 성분 분석, 항산화 활성, 혈전 생성과 관련된 항응고 활성 및 혈소판 응집저해 활성을 평가하였다. 그 결과, 착즙액과 폴리페놀 및 플라보노이드 고함유 분획인 EA 분획물에서 강력한 DPPH 음이온, ABTS 양이온, nitrite 소거능과 환원력을 확인하였다. EA 분획의 $RC_{50}$ (활성 radical을 50% 제거하는 데 소요되는 시료의 양)는 각각 136.3, 66.2 및 $115.5{\mu}g/ml$ 값을 나타내어 vitamin C가 나타내는 $RC_{50}$의 각각 1/10, 1/16 및 1/7.7에 해당하는 항산화력을 나타내었다. 또한 착즙액과 이의 EA 분획물, butanol 분획물은 아스피린에 필적하는 강력한 항응고 활성을 나타내었으며, 특히 EA 분획물과 butanol 분획물은 아스피린보다 우수한 혈소판 응집억제활성을 나타내었다. 혈소판 응집을 50% 저해할 수 있는 아스피린 농도는 0.395 mg/ml인 반면, EA 분획 및 butanol 분획은 각각 0.192 및 0.261 mg/ml로 나타났다. 상기의 활성 분획물은 0.5 mg/ml 농도까지 인간 적혈구에 대한 용혈활성을 나타내지 않았다. 이러한 결과는 블랙커런트의 강력한 항산화, 항응고, 혈소판 응집저해 활성을 이용한 신규의 항혈전제 개발 및 이용이 가능함을 제시하고 있다. 본 연구는 블랙커런트의 항혈전 활성에 대한 최초의 보고이다.

Keywords

References

  1. Ahn, S. M., Ryu, H. Y., Kang, D. K., Jung, I. C. and Sohn, H. Y. 2009. Antimicrobial and antioxidant activity of the fruit of Prunus avium L. Kor. J. Microbiol. Biotechnol. 36, 195-200. https://doi.org/10.1007/s10295-008-0485-8
  2. Benn, T., Kim, B., Park, Y. K., Wegner, C. J., Harness, E., Nam, T. G. and Kim, D. O., Lee, J. S. and Lee, J. Y. 2014. Polyphenol rich blackcurrant extract prevents inflammation in diet-induced obsess mice. J. Nutr. Biochem. 25, 1019-1025. https://doi.org/10.1016/j.jnutbio.2014.05.008
  3. Bijak, M., Bobrowski, M., Borowiecka, M., Podsedek, A., Golanski, J. and Nowak, P. 2011. Anticoagulant effect of polyphenols- rich extracts from black chokeberry and grape seeds. Fitoterapia 82, 811-817. https://doi.org/10.1016/j.fitote.2011.04.017
  4. Bijak, M., Nowak, P., Bobrowiecka, M., Ponczek, M., Zbikowska, H. and Wacowicz, B. 2012. Protective effects of (-)-epicatechin against nitrative modifications of fibrinogen. Thrombosis Res. 130, 123-128.
  5. Chen, H., Qi, X., He, C., Yin, Z., Fan, D. and Han, G. 2013. Coagulation imbalance may not contribute to the development of portal vein thrombosis in patients with cirrhosis. Thrombosis Res. 131, 173-177. https://doi.org/10.1016/j.thromres.2012.11.003
  6. Denev, P., Kratchanova, M., Ciz, M., Lojek, A., Vasicek, O., Nedelcheva, P., Blazheva, D., Toshkova, R., Gardeva, E., Yossifova, L., Hyrsl, P. and Vojtek, L. 2014. Biological activities of selected polyphenol-rich fruits related to immunity and gastrointestinal health. Food Chem. 157, 37-44. https://doi.org/10.1016/j.foodchem.2014.02.022
  7. Gavrilova, V., Kajdzanoska, M., Gjamovski, V. and Stefova, M. 2011. Separation, characterization and quantification of phenolic compounds in blueberries and red and black currants by HPLC-DAD-ESI-MS. J. Agric. Food Chem. 59, 4009-4018. https://doi.org/10.1021/jf104565y
  8. Ghosh, D., McGhie, T. K., Fisher, D. R. and Joseph, J. A. 2007. Cytoprotective effects of anthocyanins and other phenolic fractions of boysenberry and black currant on dopamine and amyloid ${\beta}$-induced oxidative stress in transfected COS-7 cells. J. Sci. Food Agric. 87, 2061-2067. https://doi.org/10.1002/jsfa.2964
  9. Ghosh, D., McGhie, T. K., Zhang, J., Adaim, A. and Skinner, M. 2006. Effects of anthocyanins and other phenolics of boysenberry and black currant as inhibitors of oxidative stress and damage to cellular DNA in SH-SY5Y and HL-60 cells. J. Sci. Food Agric. 86, 678-686. https://doi.org/10.1002/jsfa.2409
  10. Huebbe, P., Griller, K., de Pascual-Teresa, S., Arkenau, A., Adolphi, B., Portius, S. and Arkenau, C. N. 2012. Effects of black currant-based juice on atherosclerosis-related biomarkers in cultured macrophages and in human subjects after consumption of a high-energy meal. Br. J. Nutr. 108, 234-244. https://doi.org/10.1017/S0007114511005642
  11. Jeong, C. H., Jang, C. W., Lee, K. Y., Kim, I. H. and Shim, K. H. 2012. Chemical components and anti-oxidant activities of black currant. Kor. J. Food Preserv. 19, 263-270. https://doi.org/10.11002/kjfp.2012.19.2.263
  12. Kessler, T., Jansen, B. and Hesse, A. 2002. Effect of black currant-, cranberry- and plum juice consumption on risk factor associated with kidney stone formation. Eur. J. Clin. Nutr. 56, 1020-1023. https://doi.org/10.1038/sj.ejcn.1601442
  13. Kim, M. S. and Sohn, H. Y. 2014. Anti-thrombosis activity of the aerial parts of Aruncus dioicus var kamtschaticus. J. Life Sci. 24, 515-521. https://doi.org/10.5352/JLS.2014.24.5.515
  14. Lala, G., Malik, M., Zhao, C., He, J., Kwon, Y., Giusti, M. M. and Magnuson, B. A. 2006. Anthocyanin-rich extracts inhibit multiple biomarkers of colon cancer in rats. Nutr. Cancer 54, 84-93. https://doi.org/10.1207/s15327914nc5401_10
  15. Lu, Y. and Foo, Y. 2003. Polyphenolics constituents of black currant seed residue. Food Chem. 80, 71-76. https://doi.org/10.1016/S0308-8146(02)00239-X
  16. Martinez, M., Weisel, J. W. and Ischiropoulos, H. 2013. Functional impact of oxidative posttranslational modifications on fibrinogen and fibrin clots. Free Radic. Biol. Med. 65, 411-418. https://doi.org/10.1016/j.freeradbiomed.2013.06.039
  17. Miller, N. J. and Rice-Evans, C. A. 1997. The relative contributions of ascorbic acid and phenolic antioxidants to the total antioxidant activity of orange and apple fruit juices and black currant drink. Food Chem. 60, 331-337. https://doi.org/10.1016/S0308-8146(96)00339-1
  18. Parkar, S. G., Redgate, E. L., McGhie, T. K. and Hurst, R. D. 2014. In vitro studies of modulation of pathogenic and probiotic bacterial proliferation and adhesion to intestinal cells by blackcurrant juices. J. Funct. Foods 8, 35-44. https://doi.org/10.1016/j.jff.2014.02.021
  19. Ryu, H. Y., Ahn, S. M., Kim, J. S. and Sohn, H. Y. 2010. Evaluation of in-vitro anticoagulation activity of 33 different medicinal herbs. J. Life Sci. 20, 922-928. https://doi.org/10.5352/JLS.2010.20.6.922
  20. Singleton, V. L., Orthofer, R. and Lamuela-Raventos, R. M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaleau reagent. Methods Enzymol. 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
  21. Seri, S. and D'Alessandro, A. 1997. Blackcurrant seed oil, zinc, and fetal alcohol syndrome. J. Biol. Res. 73, 15-21
  22. Strathearn, K. E., Yousef, G. G., Grace, M. H., Roy, S. L., Tambe, M. A., Ferruzzi, M. G., Wu, Q. L., Simon, J. E., Lila, M. A. and Rochet, J. C. 2014. Neuroprotective effects of anthocyanins- and proanthocyanidin-rich extracts in cellular models of parkinson's disease. Brain Res. 1555, 60-77. https://doi.org/10.1016/j.brainres.2014.01.047
  23. Sweeney, J. D., Hoerning, L. A., Behrens, A. N., Novak, E. and Swank, R. T. 1990. Thrombocytopenia after desmopressin but absence of in-vitro hypersensitivity to ristocetin. Amer. J. Clin. Pathol. 93, 522-525 https://doi.org/10.1093/ajcp/93.4.522
  24. Sweeney, J. D., Hoerning, L. A. and Fitzpatrick, J. E. 1989. Whole blood aggregation in Von willebrand disease. Amer. J. Hematol. 32, 190-193. https://doi.org/10.1002/ajh.2830320306
  25. Szachowicz-Petelska, B., Dobrzynska, I., Skrzydlewska, E. and Figaszewski, Z. 2012. Protective effect of blackcurrant on liver cell membrane of rats intoxicated with ethanol. J. Memb. Biol. 245, 191-200. https://doi.org/10.1007/s00232-012-9429-3
  26. Tabart, J., Kevers, C., Pincemail, J., Defraigen, J. O. and Dommes, J. 2006. Antioxidant capacity of black currant varies with organ, season, and cultivar. J. Agric. Food Chem. 54, 6271-6276. https://doi.org/10.1021/jf061112y
  27. Valentina, U., Fabcic, J. and Stampar. F. 2007. Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chem. 107, 185-192.
  28. Wang, L. Y., Tong, D., Zhang, J. M., Ma, L., Zhang, Y., Zhao, C. and Yan, X. 2013. Study on anti-avian leukosis virus subgroup A activity of anthocyanin from a blackcurrant. J. Food Sci. Biotechnol. 32, 821-827.
  29. Yook, C., Seo, M. H., Kim, D. H. and Kim, J. S. 2007. Quality improvement of Campbell early wine by mixing with different fruits. Kor. J. Food Sci. Technol. 39, 390-399.