한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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LC-MS/MS를 이용한 식품 중 천속단과 한속단 지표성분 동시분석

Simultaneous determination of Phlomis umbrosa and Dipsacus asperoides in foods using LC-MS/MS methods

  • 윤지숙 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 김진영 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 최장덕 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 권기성 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀) ;
  • 조천호 (식품의약품안전처 식품의약품안전평가원 신종유해물질팀)
  • Yun, Jisuk (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Kim, Jinyoung (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Choi, Jangduck (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Kwon, Kisung (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Jo, Cheon-Ho (New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
  • 투고 : 2016.06.08
  • 심사 : 2016.09.01
  • 발행 : 2016.12.31
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초록

천속단과 한속단은 명칭이 유사하여 '속단'으로 통칭하여 판매되고 있으나, 종과 속이 서로 다른 약재일 뿐만 아니라 함유 성분이 다르므로 주의가 필요하다. 천속단은 골격강화, 골절치료 작용이 있는 한약재로 식품원료로는 사용할 수 없으나, 한속단의 뿌리와 잎은 식품원료로 사용이 가능하다. 본 연구에서는 식품 중 천속단 혼입여부 판별을 위하여 LC-MS/MS를 이용한 동시분석법을 개발하였으며, 선택성, 직선성, 검출한계, 정량한계, 정확성, 정밀성을 검토하였다. 국내 유통중인 식품 중 어린이 키성장을 표방하는 제품 17건을 구매하여 천속단 혼입여부에 대한 실태조사를 하였다. 분석결과 17건의 시료 중 5건의 시료에서 천속단이 검출되었으며, 천속단 지표성분의 함량은 loganin $0.19-14.45{\mu}g/mL$, sweroside $0.13-4.61{\mu}g/mL$, akebia saponin D $0.59-19.29{\mu}g/mL$이었다. 본 연구에서 개발된 동시분석법은 식품 중 불법 혼입된 천속단을 간단하면서도 신속하게 판별하는데 도움이 될 것으로 사료된다.

Phlomis umbrosa and Dipsacus asperoides are distinct species, even though they have a similar appearance. Dipsacus asperoides is used as a Chinese medicinal plant for and has bone strengthening and fracture healing but Phlomis umbrosa has no effect on bone growth. Recently, these plants were used in children's food to improve their bone growth, without distinction in food. Intakes of Dipsacus asperoides in food may be dangerous, because it has never been used in food and its safety has never been tested in humans. We developed liquid chromatography with tandem mass spectrometry method to distinguish these plants in food. The method was validated for linearity, limits of detection, limits of quantification, accuracy and precision. In 5 of 17 samples, we identified Dipsacus asperoides, containing loganin $0.19-14.45{\mu}g/mL$, sweroside $0.13-4.61{\mu}g/mL$ and akebia saponin D $0.59-19.29{\mu}g/mL$. The developed method might be useful to identify Dipsacus asperoides in adulterated food.

키워드

참고문헌

  1. Korean Ministry of Food and drug Safety (MFDS). The Korean Herbal Pharmacopoeia. Available from: https://www.mfds.go.kr/herbmed/index.do?nMenuCode=7&code=KHP-N348&includeUrl=/herbmed/view.jsp. Accessed Aug. 5, 2016.
  2. Lee MY, Ryuk JA, Kim HJ, Kim YH, Chae BC, Ko BS. Discrimination of Phlomidis radix and Dipsaci radix using the random amplified polymorphic DNA anlysis. Korean J. Oriental Med. 13: 147-152 (2007)
  3. Cho HE, Son IS, Kim SC, Son KH, Woo MH, Moon DC. Simultaneous determination of asperosaponins and iridoid glycosides from Dipsaci radix by using LC-ESI-MS sperctrometry. Korean J. Physiol. Pha. 42: 137-146 (2012)
  4. KIOM. Korean Medicinal Materials. Korea Institute of Oriental Medicine, Daejeon, Korea. pp. 204-211 (2014)
  5. Korean Ministry of food and drug safety (MFDS). Food material database. Available from: http://www.foodsafetykorea.go.kr/portal/safefoodlife/foodMeterial/foodMeterialDBDetail.do?menu_no=294&menu_grp=MENU_GRP01&=&search_type=1&animal=&indx=18179&page=1&srch_name=&edible=&=&query=%ED%95%9C%EC%86%8D%EB%8B%A8&=&=. Accessed Jun. 7, 2016.
  6. Park JH, Bae JY, Ahn MJ. Pharmacognostical studies on the 'SogDan' from Korea. Korean J. Physiol. Pha. 40: 286-288 (2009)
  7. Baek JM, Park JH, Song JH, Park SN, Jung WJ, Moon SY, Lee CH, Choi MG, Kim JJ. The effects of Phlomis umbrosa Trucz on osteoclast differentiation. Korea J. Phys. Anthropol. 26: 115-123 (2013) https://doi.org/10.11637/kjpa.2013.26.3.115
  8. Wong RW, Rabie AB, Hagg EU. The effect of crude extract from Radix dipsaci on bone in mice. Phytother. Res. 21: 596-598 (2007) https://doi.org/10.1002/ptr.2126
  9. Shang X, Wang J, Li M, Miao X, Pan H, Yang Y, Wang Y. Antinociceptive and anti-inflammatory activities of Phlomis umbrosa Turcz extract. Fitoterapia. 82: 716-721 (2011) https://doi.org/10.1016/j.fitote.2011.03.001
  10. Ling Y, Liu K, Zhang Q, Liao L, Lu Y. High performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight-mass spectrometry as a powerful analytical strategy for systematic analysis and improved characterization of the major bioactive constituents from Radix dipsaci. J. Pharmaceut. Biomed. 98: 120-129 (2014) https://doi.org/10.1016/j.jpba.2014.05.006
  11. Huang YX, Liu EW, Wang L, Huo Y, Wang Q, Olaleye O, Wang T, Gao XM. LC/MS/MS determination and pharmacokinetic studies of six compounds in rat plasma following oral administration of the single and combined extracts of Eucommia ulmoides and Dipsacus asperoides. Chin. J. Nat. Med. 12: 469-476 (2014)
  12. Zhao BT, Jeong SY, Moon DC, Son KH, Son JK, Woo MH. Quantitative determination and pattern recognition analyses of bioactive marker compounds from Dipsaci radix by HPLC. Arch. Pharm. Res. 36: 1345-1353 (2013) https://doi.org/10.1007/s12272-013-0162-y
  13. Lee DH, Kim YS, Song JB, Kim HS, Lee HJ, Guo HL, Kim HC. Effects of Phlomis umbrosa root on longitudinal bone growth rate in adolescent female rats. Molecules 21: 461 (2016) https://doi.org/10.3390/molecules21040461
  14. Wu Y, Ji D, Liu Y, Zhang C, Yang Z. Industrial-scale preparation of akebia saponin D by a two-step macroporous resin column separation. Molecules 17: 7798-7809 (2012) https://doi.org/10.3390/molecules17077798
  15. Korean Ministry of food and drug safety (MFDS). Korean Health Functional Standard Code. Available from: http://www.foodsafetykorea.go.kr/portal/healthyfoodlife/searchHomeHFDetail.do. Accessed Aug. 4, 2016.
  16. Association of Official Analytical Chemists (AOAC). Appendix K: Guidelines for Dietary Supplements and Botanicals. Available from: http://www.aoac.org/aoac_prod_imis/AOAC_Docs/StandardsDevelopment/eoma_appendix_k.pdf. Accessed May 20, 2016.
  17. Lee JK, Lee DG, Kwak BY, Kim JS, Yi K. Effect of combined extract of Hansogdan (Phlomis umbrosa) and Dalgaebi (Commeline communis) as a milk additive for enhancing the growth of physical height in vivo. Food Sci. Biotechnol. 21: 875-879 (2012) https://doi.org/10.1007/s10068-012-0113-2
  18. Kim MY, Kim JY, Lim DW, Lee DH, Kim YJ, Chang GT, Choi HY, Kim HC. Skeletal growth and IGF levels in rats after HT042 treatment. Phytother. Res. 26: 1771-1778 (2012) https://doi.org/10.1002/ptr.4642
  19. US Food and Drug Administration (FDA). FDA Poisonous Plant Database. Available from: http://www.accessdata.fda.gov/scripts/plantox/detail.cfm?id=11513. Accessed Jun. 3, 2016.
  20. Kim KH, Kim YS, Kim MR, Lee HY, Lee KH, Kim JH, Seong RS, Kang TS, Lee JH, Jang YM. Development of primer sets for the detection of Polygonum multiflorum Cynanchum wilfordii and C. auriculatum. J. Fd. Hyg. Safety. 30: 1-6 (2015) https://doi.org/10.13103/JFHS.2015.30.1.1