Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
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A Distinguishing Staining Methods for Vitality of Kudoa (Kudoa septempunctata) spores in Muscle of Olive flounder (Paralichthys olivaceus)

넙치에 기생하는 쿠도아 점액포자충(Kudoa septempunctata) 의 활성 확인을 위한 염색법의 비교

  • DO, Jeong-Wan (Pathology Division of National Institute Fisheries Science) ;
  • MOON, Sun Hwa (Pathology Division of National Institute Fisheries Science) ;
  • KIM, Min Ji (Pathology Division of National Institute Fisheries Science) ;
  • CHO, Mi-Young (Pathology Division of National Institute Fisheries Science) ;
  • JUNG, Sung Hee (Pathology Division of National Institute Fisheries Science) ;
  • LEE, Nam-Sil (Pathology Division of National Institute Fisheries Science)
  • 도정완 (국립수산과학원 병리연구과) ;
  • 문선화 (국립수산과학원 병리연구과) ;
  • 김민지 (국립수산과학원 병리연구과) ;
  • 조미영 (국립수산과학원 병리연구과) ;
  • 정승희 (국립수산과학원 병리연구과) ;
  • 이남실 (국립수산과학원 병리연구과)
  • Received : 2016.09.20
  • Accepted : 2016.11.25
  • Published : 2016.12.31
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Abstract

Consumption of Paralichthys olivaceus in raw fish have been reported as the cause of outbreaks of food-born illness, and Kudoa septempunctata in muscle of Paralichtys olivaceus was suggested with the causative agent. For this reason, distinguish of vital and dead spore is important to study survivability of Kudoa septempunctata in human intestinal condition and in vitro inactivation of Kudoa septempunctata. In this reports, we suggest NR & MB (Neutral red and Methylene blue) staining method that is easier and simpler than the previously described HO & PI (Hoechst33342 and Propidium iodide) method according to a experimental condition.

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References

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