Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
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Disease Resistance against Bacterial Infection on Treatment of Hot-water Extract with 6 Herbal Mixtures in Olive Flounder Paralichthys olivaceus

복합 생약재 열수추출물 투여에 따른 넙치 세균성 질병에 대한 질병저항성

  • Received : 2016.09.02
  • Accepted : 2016.09.26
  • Published : 2016.12.31
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Abstract

The heat extracts of six kinds of medicinal herbs (Scutellaria baicalensis, Sophora flavescens, Citrus unshiu pericarpium, Lonicera japonica, Perilla frutescens, Benincasa hispida) were tested for non-specific immune response and disease resistance effects related with fish diseases on olive flounder, Paralichthys olivaceus. The preventive effects of 6 herbal mixtures against bacterial disease on cultured flounder were examined follow as feeding EP absorbed with the heat extract of six kinds of medicinal herbs. For feeding trial for 12th week, weight gain and serum analysis of fish fed various groups were not significant differences. Lysozyme activity of the 0.01 % treated group on 4th week showed significant increase. Histopathology of the administrated group for feeding period showed no particular signs of tissue degeneration. At 0.01% oral experimental group, relative percent survival (RPS) were only 50% to 75% for four weeks and eight weeks group by intraperitoneal injection with E. tarda. The results suggest that heat extracts of six kinds of medicinal herbs (0.01%) would be effective to enhance the nonspecific immunity and protective ability of olive flounder against fish pathogen.

복합 생약재 열수추출물(6종 혼합)을 농도별 기간별로 사료에 흡습시켜 12주 동안 넙치에 급여 후 비특이적 면역 및 질병저항성에 대한 효과를 조사하였다. 복합 생약재 열수추출물의 첨가는 어류 성장에는 아무런 영향을 미치지 않았으며 혈액생화학적인, 결과도 넙치의 생리에는 아무런 영향을 미치지 않았다. 라이소자임 활성은 0.01%의 복합 생약재 열수추출물 농도로 흡습시킨 사료 그룹에서 라이소자임 활성이 증가됨을 알 수 있었다. 복합 생약재 열수추출물의 투여에 따른 넙치 장기의 병리조직에 영향이 없음을 알 수 있었다. 에드와드균의 인위감염에 의한 질병저항성은 0.01% 복합 생약재 열수추출물 농도에서 상대생존율이 높게 나타났다. 이상의 결과를 종합하여 볼 때 넙치에 복합 생약재 열수추출물을 투여시에는 어류의 비특이적 면역력 증강 및 질병 저항성 증강을 나타내는 것으로 사료되었다.

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References

  1. Ardo, L. Yin, G. Xu, P. Varadi, L. Szigeti, G. Jeny, Z. & Jeny, G.(2008). Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of nole tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila. Aquaculture 275, 26-33. https://doi.org/10.1016/j.aquaculture.2007.12.022
  2. Cho, S. H. Lee, S. M. & Kwon, M. G.(2010). Effect of various concentrations of garlic powder and garlic extract in the diets on growth, serum chemistry and immune response of juvenile olive flounder Paralichthys olivaceus. Journal of Fish Pathology 23, 409-420.
  3. Choi, Hye-Sung et al.(2004). Antibacterial activities of hot-water and ethyl alchol extracts of medicinal herbs on fish pathogenic bacteria, Journal of Fish Pathology 17, 39-55.
  4. Choi, Hye-Sung et al.(2005). Antibacterial activities of Galla Rhois extracts against fish pathogenic bacteria, Journal of Fish Pathology 18, 239-245.
  5. Christybapita, D. Divyagnaneswari, M. & Michael, R.D.(2007). Oral administration of Eclipta alba leaf aqueous extract enhances the non-specific immune responses and disease resistance of Orechromis niloticus. Fish & Shellfish Immunology 23, 840-852. https://doi.org/10.1016/j.fsi.2007.03.010
  6. Galina, J. Yin, G. Ardo, L. & Jeney, Z.(2009). The use of immunostimulating herbs in fish. An overview of research. Fish Physiology and Biochemistry 35, 669-676. https://doi.org/10.1007/s10695-009-9304-z
  7. Harikrishnan, R. Balasundaram, C. Kim, M. C. Kim, J. S. Han, Y. J. & Heo, M. S.(2009). Innate immune response and disease resistance in Carassius auratus by triherbal solvent extracts. Fish & Shellfish Immunology 27, 508-515. https://doi.org/10.1016/j.fsi.2009.07.004
  8. Harikrishnan, R. Balasundaram, C. and Heo, M.S.(2010). Herbal supplementation diets on hematology and innate immunity in goldfish against Aeromonas hydrophila. Fish & Shellfish Immunology 28, 354-361. https://doi.org/10.1016/j.fsi.2009.11.013
  9. Hwang, Jee-Youn et al.(2015). Effect of Herbs on Vaccine Efficacy to Olive flounder, Paralichthys olivaceus. JFMSE 27, 1491-1498. https://doi.org/10.13000/JFMSE.2015.27.5.1491
  10. Jee, Bo-Young et al.(2012). Effects of various concentrations of Skullcap extracts in the diets on disease resistance of olive flounder Paralichthy olivaceus. Journal of Fish Pathology 25, 21-30. https://doi.org/10.7847/jfp.2012.25.1.021
  11. Jung, Sung-Hee et al.(2001). In vitro effect of water extract of medicinal herbs on antimicrobial activity against fish pathogenic bacteria and superoxide production of kidney phagocytes in olive flounder, Paralichthys olivaceus. Journal of Fish Pathology 14, 3-10.
  12. Lee, Jun-Hee et al.(2010). Effects of garlic Allium sativum on the immune responses of olive flounder Paralichthys olivaceus. Journal of Fish Pathology 23, 69-83.
  13. Lee, Nam-Sil et al.(2010). Anti-fish Pathogenic Efficacy of hot water extracts obtained from 5 Herbs in-vitro, and efficacy and toxicity in flounder of the one selected herb, Skullcap, Journal of Fish Pathology 23, 137-143.
  14. Parry, R. M. Chandau, R. C. & Shahani, R. M.(1965). A rapid and sensitive assay of muramidase. Proceedings of Society for Experimental Biology Medicine 119, 384-386.
  15. Robertsen, B. Engstad, R. E. & Jorgensen, J. B.(1994). $\beta$-glucans as immunostimulants. In: Stolen, S.J. and Fletcher, T.C. (eds) Modulators of Fish Immune Response (vol 1), p83-99 SOS publication, Fair Haven, U.K.
  16. Seo, Jung-Soo et al.(2015). Effect of Disease Resistance on Oral Administration of Lightyellow Sophora Extract in Olive Flounder. JFMSE 27, 1656-1664. https://doi.org/10.13000/JFMSE.2015.27.6.1656
  17. Woo, Sung-Ho et al.(2010). Effects of Galic Allium sativum extract immersion on the immune response of olive flouder Paralichthys olivaceus prechallenged with pathogenic bacteria. Journal of Fish Pathology 23, 199-209.
  18. Wu, Y. R. Gong, Q. F. Fang, H. Liang, W. W. Chen, M. & He, R. J.(2013). Effect of Sophora flavescens on non-specific immune response tilapia (GIFT Orechromis niloticus) and disease resistance against Streptococcus agalactiae. Fish & Shellfish Immunology 34, 220-227. https://doi.org/10.1016/j.fsi.2012.10.020