Advances in environmental research

  • 제5권3호
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  • Pages.179-187
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  • 2016
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  • 2234-1722(pISSN)
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  • 2234-1730(eISSN)
테크노프레스 (Techno-Press)
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DOI QR코드

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Induction of oxidative stress in Clarias gariepinus from Eleyele River in Nigeria

  • Arojojoye, Oluwatosin A. (Department of Biochemistry, Faculty of Sciences, Lead City University) ;
  • Nwaechefu, Olajumoke O. (Department of Biochemistry, Faculty of Sciences, Lead City University) ;
  • Ajiboye, John A. (Department of Chemical Sciences, Biochemistry unit, College of Natural and Applied Sciences, Bells University of Technology) ;
  • Akintunde, Jacob K. (Department of Environmental Health Sciences, Faculty of Public Health, University College Hospital)
  • 투고 : 2016.08.26
  • 심사 : 2016.10.28
  • 발행 : 2016.09.25
⟨ 이전 논문 다음 논문 ⟩

초록

This study evaluated some markers of oxidative stress in the organs of African Catfish, Clarias gariepinus from Eleyele River in Oyo State, Nigeria. Clarias gariepinus (250 g-400 g) were collected from Eleyele River (a suspected polluted River) and Clarias gariepinus from a clean fish farm (Durantee fisheries) were used as the control. Levels of Malondialdehyde (index of lipid peroxidation), Glutathione (GSH) and activities of antioxidant enzymes- Superoxide dismutase (SOD), Catalase and Glutathione-S-Transferase (GST) were evaluated in the liver, kidney and gills of the fish. From the results, there were significant (p<0.001) increases in malondialdehyde and GSH levels in the liver, kidney and gills of Clarias gariepinus from Eleyele River compared with control. The activity of GST increased significantly (p<0.05; p<0.001) in the liver and kidney of fish from Eleyele River compared with control. There was a significant decrease (p<0.05; p<0.001) in SOD activity in all the organs of Clarias gariepinus from Eleyele River compared with conrol and also a significant (p<0.001) decrease in catalase activity in the gills and kidney of the fish but catalase activity increased in the liver. Increase in lipid peroxidation and alterations in antioxidant status in Clarias gariepinus from Eleyele River show that the fish were under oxidative stress. These suggest that the River is polluted probably as a result of various wastes frequently discharged into the River. This could pose serious health risks to consumers of water and aquatic organisms from the River.

키워드

참고문헌

  1. Akinyemi, L.P., Odunaike, R.K., Daniel, D.E. and Alausa, S.K. (2014), "Physico-chemical parameters and heavy metals concentrations in Eleyele River in Oyo State, South-West of Nigeria", Int. J. Environ. Sci. Toxicol. Res., 2(1), 1-5.
  2. Almeida, J.A., Diniz, Y.S., Marques, S.F.G., Faine, L.A., Ribas, B.O., Burneiko, R.C. and Novelli, E.L.B. (2002), "The use of the oxidative stress responses as biomarkers in Nile tilapia (Oreochromis niloticus) exposed to in vivo cadmium contamination", Environ. Int., 27(8), 673-679. https://doi.org/10.1016/S0160-4120(01)00127-1
  3. Bacanskas, L.R., Whitaker, J. and Di Giulio, R.T., (2004), "Oxidative stress in two populations of killifish (Fundulus heteroclitus) with differing contaminant exposure histories", Marine Environ. Res., 58(2-5), 597-601. https://doi.org/10.1016/j.marenvres.2004.03.048
  4. Borkovic, S.S., saponjic, J.S., Pavlovic, S.Z., Blagojevic, D.P., Milosevic, S.M., Kovacevic, T.B. and Saicic, Z.S. (2005), "The activity of antioxidant defence enzymes in the mussel Mytilus galloprovincialis from the Adriatic Sea", Comparative Biochem. Physiol. Part C: Toxicol. Pharmacol., 141(4), 366-374. https://doi.org/10.1016/j.cbpc.2005.08.001
  5. Dautremepuits, C., Paris-Palacios, S., Betoulle, S. and Vernet, G. (2004), "Modulation in hepatic and head kidney parameters of carp (Cyprinus carpio L.) induced by copper and chitosan", Comparative Biochem. Physiol. Part C: Toxicol. Pharmacol., 137(4), 325-333. https://doi.org/10.1016/j.cca.2004.03.005
  6. Drake, J., Sultana, R., Aksenova, M., Calabrese, V. and Butter field, D.A. (2003), "Elevation of mitochondrial glutathione by gamma-glutamylcysteine ethyl ester protects mitochondria against peroxynitrite-induced oxidative stress", J. Neurosci. Res., 74(6), 917-927. https://doi.org/10.1002/jnr.10810
  7. Falfushynska, H. and Stolyar, O. (2009), "Responses of biochemical markers in carp Cyprinus carpio from two field sites in Western Ukraine", Ecotoxicology and EnvironmentalSafety, 72(3), 729-736.
  8. Falfushynska, H., Gnatyshyna, L., Priydun, C.H., Stoliar, O. and Nam, Y.K. (2010a), "Variability of responses in the crucian carp Carassius carassius from two Ukrainian ponds determined by multi-marker approach", Ecotoxicol. Environ. Saf., 73(8),1896-1906. https://doi.org/10.1016/j.ecoenv.2010.08.029
  9. Falfushynska, H., Gnatyshyna, L., Shymkiv, T. and Stoliar, O. (2010b), "Reactivity of metallothioneins and the efficiency of their metal-keeping and antioxidative functions in the adaptation of fish Carassius carassius to field pollution", Proceeding of the Shevchenko Scientific Society. Chemistry & Biochemistry, 25, 229-242.
  10. Farombi, E.O., Adelowo, O.A. and Ajimoko, Y.R. (2007), "Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in African Cat Fish (Clarias gariepinus) from Nigeria Ogun River", Int. J. Environ. Res. Pub. Hlth., 4(2), 158-165. https://doi.org/10.3390/ijerph2007040011
  11. Ferreira, M., Moradas-Ferreira, P. and Reis-Henriques, M.A. (2005), "Oxidative stress biomarkers in two resident species, Mullet (Mugil cephalus) and Flounder (Platichthys flesus), from a polluted site in River Douro Estuary, Portugal", Aquatic Toxicol., 71(1), 39-48. https://doi.org/10.1016/j.aquatox.2004.10.009
  12. Gornall, A.G., Bardawil, C.J. and David, M.M. (1949), "Determination of serum proteins by means of the Biuret reagent", J. Biol. Chem., 177(2), 751-756.
  13. Habig, W.H., Pabst, M.J. and Jacoby, W.B. (1974), "Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation", J. Biol. Chem., 249(22), 7130-7139.
  14. Halliwell, B. and Gutteridge, J.M.C. (1989), Free radicals in biology and medicine, (2nd edition), Clarendon Press, Oxford, 543.
  15. Huang, D.J., Zhang, Y.M., Song, G, Long, J., Liu, J.H. and Ji, W.H. (2007), "Contaminants-induced oxidative damage on the Carp Cyprinus carpio collected from the upper Yellow River, China", J. Environ. Monit. Assess., 128(1-3), 483-488. https://doi.org/10.1007/s10661-006-9341-3
  16. Jollow, D.J., Michel, J.R., Zampaglione, N. and Gillete, J.R. (1974), "Bromobenzene induced liver Necrosis: Protective role of GSH and evidence for 3, 4 -Bromobenzene oxide as the Hepatotoxic metabolite", Pharmacol., 11(3), 151-169. https://doi.org/10.1159/000136485
  17. Kadar, E., Costa, V. and Santos, R.S. (2005), "Distribution of micro-essential (Fe, Cu, Zn) and toxic (Hg) metals in tissues of two nutritionally distinct hydrothermal shrimps", Sci. Total Environ., 358(1), 143-150. https://doi.org/10.1016/j.scitotenv.2005.09.003
  18. Lopes, P.A., Pinheiro, T., Santos, C.M., Da Luz Mathias, M., Collares-Pereira, J.M. and Viegas-Crespo, M.A. (2001), "Response of antioxidant enzymes in freshwater fish populations (Leuciscus alburnoides complex) to inorganic pollutants exposure", Sci. Total Environ., 280(1), 153-63. https://doi.org/10.1016/S0048-9697(01)00822-1
  19. Lushchak, V.I., Lushchak, L.P., Mota, A.A. and Hermes-Lima, M. (2001), "Oxidative stress and antioxidant defenses in goldfish Carassius auratus during anoxia and reoxygenation", Am. J. Physiol.-Regulatory, Integrat. Comper. Physiol., 280(1), 100-107. https://doi.org/10.1152/ajpregu.2001.280.1.R100
  20. Magwere, T., Naik, Y.S. and Hasler, J.A. (1997), "Effect of chloroquine treatment on antioxidant enzymes in rat liver and kidney", Free Rad. Biol. Med., 22(1), 321-327. https://doi.org/10.1016/S0891-5849(96)00285-7
  21. Masella, R., Di Benedetto, R., Varì, R., Filesi, C. and Giovannini, C. (2005), "Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes", J. Nutr. Biochem., 16(10), 577-586. https://doi.org/10.1016/j.jnutbio.2005.05.013
  22. Mates, M. (2000), "Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology", Toxicol., 153(1), 83-104. https://doi.org/10.1016/S0300-483X(00)00306-1
  23. Nishida, Y. (2011), "The chemical process of oxidative stress by copper (II) and iron (III) ions in several neurodegenerative disorders", Monatshefte fur Chemie, 142(4), 375-384. https://doi.org/10.1007/s00706-010-0444-8
  24. Nordberg, J. and Arner, E.S.J. (2001), "Reactive oxygen species, antioxidants, and the mammalian thiredoxin system", J. Free Rad. Biol. Med., 31(11), 1287-1312. https://doi.org/10.1016/S0891-5849(01)00724-9
  25. Pandey, S., Parvez, S., Sayeed, I., Haque, R., Bin-Hafeez, B. and Raisuddin, B. (2003), "Biomarkers of oxidative stress: A comparative study of River Yamuna fish Wallago attu (Bl. & Schn.)", Sci. Total Environ., 309(1), 105-115. https://doi.org/10.1016/S0048-9697(03)00006-8
  26. Reddy, P.B. (2012), "Histopathogical studies as potential and direct biomarkers of pollution", Trend. Life Sci., 1(1), 27-31.
  27. Reddy, P.B. and Rawat, S.S. (2013), "Assessment of aquatic pollution using histopathology in fish as a protocol", Int. Res. J. Environ. Sci., 2(8), 79-82.
  28. Sanchez, W., Selim, A., Olivier, P., Jean-Maxence, D. and Jean-Marc, P. (2007), "Preliminary investigation of multi-biomarker responses in three-spined stickleback (Gasteros-teus aculeatus L.) sampled in contaminated streams", Ecotoxicol., Springer Verlag (Ger-many) 16(2), 279-287. https://doi.org/10.1007/s10646-006-0131-z
  29. Sevcikova, M., Modra, H., Slaninova, A. and Svobodova, Z. (2011), "Metals as a cause of oxidative stress in fish: A review", Veterinari Medicina, 56(11), 537-554. https://doi.org/10.17221/4272-VETMED
  30. Sinha, A.K. (1972), "Colorimetric assay of catalase", Anal. Biochem., 47(2), 389-394. https://doi.org/10.1016/0003-2697(72)90132-7
  31. Slaninova, A., Smutna, M., Modra, H. and Svobodova, Z. (2009), "A review: Oxidative stress in fish induced by pesticides", Neuroendocrinol. Lett., 30(1), 2-12.
  32. Valko, M., Rhodes, C.J., Moncol, J., Izakovic, M.M. and Mazur, M. (2006), "Free radicals, metals and antioxidants in oxidative stress-induced cancer", Chemico-Biol Interac., 160(1), 1-40. https://doi.org/10.1016/j.cbi.2005.12.009
  33. Van der Oost, R., Beyer, J. and Vermeulen, N.P.E. (2003), "Fish bioaccumulation and biomarkers in environmental risk assessment: A review", Environ. Toxicol. and Pharmacol., 13, 57-149. https://doi.org/10.1016/S1382-6689(02)00126-6
  34. Varshney, R. and Kale, R.F. (1990), "Effect of Calmodulin antagonists on radiation induced lipid peroxidation in Microsomes", Int. J. Rad. Biol., 58(5), 733-743. https://doi.org/10.1080/09553009014552121
  35. Yildirim, N.C., Benzer, F. and Danabas, D. (2011), "Evaluation of environmental pollution at munzur River of tunceli applying oxidative stress biomarkers in capoeta trutta (heckel, 1843)", J. Animal Plant Sci., 21(1), 66-71.
  36. Yildirim, N. and Asma, D. (2010), "Response of antioxidant defence system to cadmium induced toxicity in white rot fungus Phanerochaete chrysosporium, Fresenius", Environ. Bull., 19(12a), 3059-3065.