Antiproliferative Evaluation and Apoptosis Induction in MCF-7 Cells by Ziziphus spina christi Leaf Extracts

  • Farmani, Fatemeh (Medicinal Plants Processing Research Center, School of Pharmacy, Shiraz University of Medical Sciences) ;
  • Moein, Mahmoodreza (Medicinal Plants Processing Research Center, School of Pharmacy, Shiraz University of Medical Sciences) ;
  • Amanzadeh, Amir (National Cell Bank of Iran, Pasteur Institute of Iran) ;
  • Kandelous, Hirsa Mostafapour (Department of Physiology and Pharmacology, Pasteur Institute of Iran) ;
  • Ehsanpour, Zahra (Department of Physiology and Pharmacology, Pasteur Institute of Iran) ;
  • Salimi, Mona (Department of Physiology and Pharmacology, Pasteur Institute of Iran)
  • Published : 2016.02.05


Background: Herbal medicine has becoming a potential source of treatment for different types of cancer including breast cancer. It has been shown that plants from the family Rhamnaceae possess anticancer activity. Objective: In this study, we determined the antiproliferative influence of Ziziphus spina christi- a species from this family- on the MCF-7 (human breast adenocarcinoma) cell line. Materials and Methods: The cytotoxicity of the total extract, ethanol, ethanol-aqueous (1:1) as well as aqueous fractions of Ziziphus spina christi leaves was evaluated through MTT assay against MCF-7 cell line. Cell cycle inhibition and apoptosis induction were assessed by flowcytometry cycle RNase/PI analysis and Annexin V-FLUOS, respectively. Apoptosis was also analyzed by immunoblotting assay. Results: Our results indicated that the ethanolic fraction had the lowest $IC_{50}$ value (0.02 mg/ml), induced cell cycle arrest at the G1/S phase as well as apoptosis after a 48h of treatment. Conclusions: This is the first report on anticancer effect of Ziziphus spina christi ethanolic fraction on breast cancer cells, providing a scientific basis for its utility in traditional medicine. However, further in-depth studies are needed to confirm the precise mechanisms.


Supported by : Shiraz University of Medical sciences


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