Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Transcript Profiling of Toll-Like Receptor mRNAs in Selected Tissues of Mink (Neovison vison)

  • Tong, Mingwei (State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences) ;
  • Yi, Li (State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences) ;
  • Cheng, Yuening (State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences) ;
  • Zhang, Miao (State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences) ;
  • Cao, Zhigang (State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences) ;
  • Wang, Jianke (State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences) ;
  • Zhao, Hang (State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences) ;
  • Lin, Peng (State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences) ;
  • Yang, Yong (Wu Xi Medical School, Jiangnan University) ;
  • Cheng, Shipeng (State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences)
  • Received : 2016.04.25
  • Accepted : 2016.07.17
  • Published : 2016.12.28
Download PDF
⟨ Previous Next ⟩

Abstract

Toll-like receptors (TLRs) can recognize conserved molecular patterns and initiate a wide range of innate and adaptive immune responses against invading infectious agents. The aim of this study was to assess the transcript profile of mink TLRs (mTLRs) in mink peripheral blood mononuclear cells (PBMCs) and a range of tissues, and to explore the potential role of mTLRs in the antiviral immune response process. The results indicated that the mTLR partial nucleotide sequences had a high degree of nucleotide identity with ferret sequences (95-98%). Phylogenetic analysis showed that mammalian TLRs grouped into five TLR families, with a closer relationship of the mTLRs with those of ferret than the other mammalian sequences. Moreover, all the mTLRs were ubiquitously expressed in lymphoid organs (spleen and lymph nodes) and PBMCs. Interestingly, the mTLR expression patterns in lung, uterus, and heart showed quite a lot of similarity. Another remarkable observation was the wide expression of mTLR1-3 mRNAs in all tissues. Among the analyzed tissues, skeletal muscle was revealed to being the lowest repertoire of mTLR expression. Additionally, mink PBMCs exposed to the canine distemper virus revealed significant upregulation of mTLR2, mTLR4, mTLR7, and mTLR8 mRNAs, indicating that mTLRs have a role in innate immunity in the mink. Collectively, our results are the first to establish the basic expression patterns of mTLRs and the relationship between mTLRs and a virus, which will contribute to better understanding of the evolution and the functions of mTLRs in the innate immune system in minks.

Keywords

References

  1. Applequist SE, Wallin RP, Ljunggren HG. 2002. Variable expression of Toll-like receptor in murine innate and adaptive immune cell lines. Int. Immunol. 14: 1065-1074. https://doi.org/10.1093/intimm/dxf069
  2. Flies AS, Maksimoski MT, Mansfield LS, Weldele ML, Holekamp KE. 2014. Characterization of Toll-like receptors 1-10 in spotted hyenas. Vet. Res. Commun. 38: 165-170. https://doi.org/10.1007/s11259-014-9592-3
  3. Fougeron D, Van Maele L, Songhet P, Cayet D, Hot D, Van Rooijen N, et al. 2015. Indirect Toll-like receptor 5-mediated activation of conventional dendritic cells promotes the mucosal adjuvant activity of flagellin in the respiratory tract. Vaccine 33: 3331-3341. https://doi.org/10.1016/j.vaccine.2015.05.022
  4. Ignacio G, Nordone S, Howard KE, Dean GA. 2005. Toll-like receptor expression in feline lymphoid tissues. Vet. Immunol. Immunopathol. 106: 229-237. https://doi.org/10.1016/j.vetimm.2005.02.022
  5. Janeway CA Jr, Medzhitov R. 2002. Innate immune recognition. Annu. Rev. Immunol. 20: 197-216. https://doi.org/10.1146/annurev.immunol.20.083001.084359
  6. Jungi TW, Farhat K, Burgener IA, Werling D. 2011. Toll-like receptors in domestic animals. Cell Tissue Res. 343: 107-120. https://doi.org/10.1007/s00441-010-1047-8
  7. Kawasaki T, Kawai T. 2014. Toll-like receptor signaling pathways. Front. Immunol. 5: 461.
  8. Machida K, Cheng KT, Sung VM, Levine AM, Foung S, Lai MM. 2006. Hepatitis C virus induces Toll-like receptor 4 expression, leading to enhanced production of beta interferon and interleukin-6. J. Virol. 80: 866-874. https://doi.org/10.1128/JVI.80.2.866-874.2006
  9. Medzhitov R, Preston-Hurlburt P, Janeway CA Jr. 1997. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature 388: 394-397. https://doi.org/10.1038/41131
  10. Menzies M, Ingham A. 2006. Identification and expression of Toll-like receptors 1-10 in selected bovine and ovine tissues. Vet. Immunol. Immunopathol. 109: 23-30. https://doi.org/10.1016/j.vetimm.2005.06.014
  11. Nalubarnba KS, Gossner AG, Dalziel RG, Hopkins J. 2007. Differential expression of pattern recognition receptors in sheep tissues and leukocyte subsets. Vet. Immunol. Immunopathol. 118: 252-262. https://doi.org/10.1016/j.vetimm.2007.05.018
  12. Nishimura M, Naito S. 2005. Tissue-specific mRNA expression profiles of human Toll-like receptors and related genes. Biol. Pharm. Bull. 28: 886-892. https://doi.org/10.1248/bpb.28.886
  13. Okui Y, Kano R, Maruyama H, Hasegawa A. 2008. Cloning of canine Toll-like receptor 7 gene and its expression in dog tissues. Vet. Immunol. Immunopathol. 121: 156-160. https://doi.org/10.1016/j.vetimm.2007.08.012
  14. Parker GA, Picut CA. 2012. Immune functioning in non lymphoid organs: the liver. Toxicol. Pathol. 40: 237-247. https://doi.org/10.1177/0192623311428475
  15. Ramasamy KT, Reddy MR, Raveendranathan DN, Murugesan S, Chatterjee RN, Ullengala R, Haunshi S. 2010. Differential expression of Toll-like receptor mRNA in White Leghorn and indigenous chicken of India. Vet. Res. Commun. 34: 633-639. https://doi.org/10.1007/s11259-010-9431-0
  16. Roach JC, Glusman G, Rowen L, Kaur A, Purcell MK, Smith KD, et al. 2005. The evolution of vertebrate Toll-like receptors. Proc. Natl. Acad. Sci. USA 102: 9577-9582. https://doi.org/10.1073/pnas.0502272102
  17. Schneider-Schaulies J, Schneider-Schaulies S. 2008. Receptor interactions, tropism, and mechanisms involved in morbillivirusinduced immunomodulation. Adv. Virus Res. 71: 173-205.
  18. Sepulcre MP, Lopez-Castejon G, Meseguer J, Mulero V. 2007. The activation of gilthead seabream professional phagocytes by different PAMPs underlines the behavioural diversity of the main innate immune cells of bony fish. Mol. Immunol. 44: 2009-2016. https://doi.org/10.1016/j.molimm.2006.09.022
  19. Takeda K, Kaisho T, Akira S. 2003. Toll-like receptors. Annu. Rev. Immunol. 21: 335-376. https://doi.org/10.1146/annurev.immunol.21.120601.141126
  20. Takeuchi O, Akira S. 2010. Pattern recognition receptors and inflammation. Cell 140: 805-820. https://doi.org/10.1016/j.cell.2010.01.022
  21. Tirumurugaan KG, Dhanasekaran S, Raj GD, Raja A, Kumanan K, Ramaswamy V. 2010. Differential expression of Toll-like receptor mRNA in selected tissues of goat (Capra hircus). Vet. Immunol. Immunopathol. 133: 296-301. https://doi.org/10.1016/j.vetimm.2009.08.015
  22. Uematsu S, Fujimoto K. 2010. The innate immune system in the intestine. Microbiol. Immunol. 54: 645-657. https://doi.org/10.1111/j.1348-0421.2010.00267.x
  23. Vahanan BM, Raj GD, Pawar RM, Gopinath VP, Raja A, Thangavelu A. 2008. Expression profile of Toll like receptors in a range of water buffalo tissues (Bubalus bubalis). Vet. Immunol. Immunopathol. 126: 149-155. https://doi.org/10.1016/j.vetimm.2008.05.027
  24. Wang J, Cheng S, Yi L, Cheng Y, Yang S, Xu H, et al. 2013. Detection of mink enteritis virus by loop-mediated isothermal amplification (LAMP). J. Virol. Methods 187: 401-405. https://doi.org/10.1016/j.jviromet.2012.11.012
  25. Yi L, Cheng Y, Zhang M, Cao Z, Tong M, Wang J, et al. 2015. Identification of a novel canine distemper virus B-cell epitope using a monoclonal antibody against nucleocapsid protein. Virus Res. 213: 1-5.
  26. Yi L, Tong M, Cheng Y, Song W, Cheng S. 2016. Phylogenetic analysis of canine parvovirus VP2 gene in China. Transbound Emerg. Dis. 63: e262-e269. https://doi.org/10.1111/tbed.12268
  27. Zarember KA, Godowski PJ. 2002. Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leukocytes in response to microbes, their products, and cytokines. J. Immunol. 168: 554-561. https://doi.org/10.4049/jimmunol.168.2.554
  28. Zhao JJ, Shi N, Sun YG, Martella V, Nikolin V, Zhu CS, et al. 2015. Pathogenesis of canine distemper virus in experimentally infected raccoon dogs, foxes, and minks. Antiviral Res. 122: 1-11. https://doi.org/10.1016/j.antiviral.2015.07.007

Cited by

  1. Investigation of TLR1-9 genes and miR-155 expression in dogs infected with canine distemper vol.79, pp.None, 2016, https://doi.org/10.1016/j.cimid.2021.101711