Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Proteomic Analysis of a Chlamydomonas reinhardtii Mutant with Enhanced Lipid Production by the Gamma Irradiation Method

  • Baek, Jaewon (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University) ;
  • Choi, Jong-il (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University) ;
  • Park, Hyun (Korea Polar Research Institute) ;
  • Lim, Sangyong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Si Jae (Department of Environmental Engineering and Energy, Myongji University)
  • Received : 2016.05.18
  • Accepted : 2016.08.25
  • Published : 2016.12.28
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Abstract

In this study, an enhanced lipid-producing mutant strain of the microalga Chlamydomonas reinhardtii was developed by gamma irradiation. To induce the mutation, C. reinhardtii was gamma irradiated at a dose of 400 Gy. After irradiation, the surviving cells were stained with Nile red. The mutant (Cr-4013) accumulating 20% more lipid than the wild type was selected. Thin-layer chromatography revealed the triglyceride and free fatty acid contents to be markedly increased in Cr-4013. The major fatty acids identified were palmitic acid, oleic acid, linoleic acid, and linolenic acid. Random amplified polymeric DNA analysis showed partial genetic modifications in Cr-4013. To ascertain the changes of protein expression in the mutant strain, two-dimensional electrophoresis was conducted. These results showed that gamma radiation could be used for the development of efficient microalgal strains for lipid production.

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