Journal of Microbiology and Biotechnology

  • 제26권12호
  • /
  • Pages.2051-2059
  • /
  • 2016
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Novel Approach of a Phage-Based Magnetoelastic Biosensor for the Detection of Salmonella enterica serovar Typhimurium in Soil

  • Park, Mi-Kyung (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Chin, Bryan A. (Materials Research and Education Center, Auburn University)
  • 투고 : 2016.09.30
  • 심사 : 2016.10.11
  • 발행 : 2016.12.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

To date, there has been no employment of a magnetoelastic (ME) biosensor method to detect Salmonella enterica serovar Typhimurium in soil. The ME biosensor method needs to be investigated and modified for its successful performance. The filtration method, cation-exchange resin method, and combinations of both methods were employed for the extraction of S. Typhimurium from soil. The number of S. Typhimurium and the resonant frequency shift of the ME sensor were then compared using a brilliant green sulfa agar plate and an HP 8751A network analyzer. A blocking study was performed using bovine serum albumin (BSA), polyethylene glycol (PEG), and casein powder suspension. Finally, the modified ME biosensor method was performed to detect S. Typhimurium in soil. The number of S. Typhimurium was significantly decreased from 7.10 log CFU/soil to 4.45-4.72 log CFU/soil after introduction of the cation-exchange resin method. The greatest resonant frequency shift of the measurement sensor was found when employing centrifugation and filtration procedures. The resonant frequency shift of the PEG-blocked measurement sensor was $3,219{\pm}755Hz$, which was significantly greater than those of the BSA- and casein-blocked ME sensor. The optimum concentration of PEG was determined to be 1.0 mg/ml after considering the resonant shift and economic issue. Finally, the modified ME biosensor method was able to detect S. Typhimurium in soil in a dose-response manner. Although these modifications of the ME biosensor method sacrificed some advantages, such as cost, time effectiveness, and operator friendliness, this study demonstrated a novel approach of the ME biosensor method to detect S. Typhimurium in soil.

키워드

참고문헌

  1. Anderson M, Jaykus LA, Beaulieu S, Dennis S. 2011. Pathogen-produce pair attribution risk ranking tool to prioritize fresh produce commodity and pathogen combinations for further evaluation ($P^3$ARRT). Food Control 22: 1865-1872. https://doi.org/10.1016/j.foodcont.2011.04.028
  2. Berger CN, Sodha SV, Shaw RK, Grifiin PM, Pink D, Hand P, Frankel G. 2010. Fresh fruit and vegetables as vehicles for the transmission of human pathogen. Environ. Microbiol. 12: 2385-2397. https://doi.org/10.1111/j.1462-2920.2010.02297.x
  3. Beuchat LR. 2002. Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables. Microbes Infect. 4: 413-423. https://doi.org/10.1016/S1286-4579(02)01555-1
  4. Beuchat LR. 2006. Vectors and conditions for preharvest contamination of fruits and vegetagles with pathogens capable of causing enteric diseases. Br. Food J. 108: 38-53. https://doi.org/10.1108/00070700610637625
  5. Byeon HM, Vodyanoy VJ, Oh JH, Kwon JH, Park MK. 2015. Lytic phage-based magnetoelastic biosensors for on-site detection of methicillin-resistant Staphylococcus aureus on spinach leaves. J. Electrochem. Soc. 162: B230-B235. https://doi.org/10.1149/2.0681508jes
  6. CDC. 2009. Surveillance for foodborne disease outbreaks— United States, 2006. MMWR Morb. Mortal. Wkly Rep. 58: 609-615.
  7. CDC. 2010. Surveillance for foodborne disease outbreaks— United States, 2007. MMWR Morb. Mortal. Wkly Rep. 59: 973-579.
  8. Chang WW, Lee CH. 2014. Salmonella as an innovative therapeutic antitumor agent. Int. J. Mol. Sci. 15: 14546-14554. https://doi.org/10.3390/ijms150814546
  9. Doyle MP, Erickson MC. 2008. The problems with fresh produce: an overview. J. Appl. Microbiol. 105: 313-330.
  10. Harris LJ, Faber JN, Beuchat LR, Parish ME, Suslow TV, Garrett EH, Busta FF. 2003. Outbreaks associated with fresh produce: incidence, growth, and survival of pathogens in fresh and fresh cut produce. Compr. Rev. Food Sci. Food Saf. 2: 78-141. https://doi.org/10.1111/j.1541-4337.2003.tb00031.x
  11. Herne TM, Tarlov MJ. 1997. Characterization of DNA probes immobilized on gold surfaces. J. Am. Chem. Soc. 119: 8916-8920. https://doi.org/10.1021/ja9719586
  12. Huang S, Yang H, Lakshmanan RS, Johnson ML, Wan J, Chen IH, et al. 2009. Sequential detection of Salmonella typhimurium and Bacillus anthracis spores using magnetoelastic biosensors. Biosens. Bioelectron. 14: 1730-1736.
  13. Islam M, Morgan J, Doyle MP, Phatak SC, Millner P, Jiang XP. 2004. Fate of Salmonella enterica serovar Typhimurium on carrots and radishes grown in fields treated with contaminated manure composts or irrigation water. Appl. Environ. Microbiol. 70: 2497-2502. https://doi.org/10.1128/AEM.70.4.2497-2502.2004
  14. Lakshmanan RS, Guntupalli R, Hu J, Petrenko VA, Barbaree JM, Chin BA. 2007. Detection of Salmonella typhimurium in fat free milk using a phage immobilized magnetoelastic sensor. Sens. Actuators B Chem. 126: 544-550. https://doi.org/10.1016/j.snb.2007.04.003
  15. Li S, Li Y, Chen H, Horikawa S, Shen W, Simonian A, Chin BA. 2010. Direct detection of Salmonella typhimurium on fresh produce using phage-based magnetoelastic biosensors. Biosens. Bioelectron. 26: 1313-1319. https://doi.org/10.1016/j.bios.2010.07.029
  16. Liu B, Huang PJJ, Zhang X, Wang F, Pautler R, Ip ACF, Liu J. 2013. Parts-per-million of polyethylene glycol as a noninterfering blocking agent for homogeneous biosensor development. Anal. Chem. 85: 10045-10050. https://doi.org/10.1021/ac4024654
  17. Park MK, Hirematha N, Weerakoon KA, Vglenov KA, Barbaree JM, Chin BA. 2013. Effects of surface morphologies of fresh produce on the performance of phage-based magnetoelastic biosensors. J. Electrochem. Soc. 160: B6-B12. https://doi.org/10.1149/2.043304jes
  18. Park MK, Li S, Chin BA. 2013. Detection of Salmonella typhimurium grown directly on tomato surface using phagebased magnetoelastic biosensors. Food Bioprocess Technol. 6: 682-689. https://doi.org/10.1007/s11947-011-0708-2
  19. Park MK, Oh JH. 2012. Rapid detection of E. coli O157:H7 on turnip greens using a modified gold biosensor combined with light microscopic imaging system. J. Food Sci. 77: M127-M134. https://doi.org/10.1111/j.1750-3841.2011.02537.x
  20. Park MK, Park JW, Wikle III HC, Chin BA. 2013. Evaluation of phage-based magnetoelastic biosensors for direct detection of Salmonella Typhimurium on spinach leaves. Sens. Actuators B Chem. 176: 1134-1140. https://doi.org/10.1016/j.snb.2012.10.084
  21. Park MK, Weerakoon KA, Oh JH, Chin BA. 2013. The analytical comparison of phage-based magnetoelastic biosensor with TaqMan-based quantitative PCR method to detect Salmonella Typhimurium on cantaloupes. Food Control 33: 330-336. https://doi.org/10.1016/j.foodcont.2013.02.026
  22. Petrenko VA, Sorokulova IB. 2004. Detection of biological threats. A challenge for directed molecular evolution. J. Microbiol. Methods 58: 147-168. https://doi.org/10.1016/j.mimet.2004.04.004
  23. Riquelme MV, Zhao H, Srinivasaraghavan V, Pruden A. 2016. Optimizing blocking of nonspecific bacterial attachment to impedimetric biosensor. Sens Biosensing Res. 8: 47-54. https://doi.org/10.1016/j.sbsr.2016.04.003
  24. Shen W, Li S, Park MK, Zhang Z, Cheng Z, Petrenko VA, Chin BA. 2012. Blocking agent optimization for nonspecific binding on phage based magnetoelastic biosensors. J. Electrochem. Soc. 159: B818-B823. https://doi.org/10.1149/2.057210jes
  25. Skogley EO, Dobermann A. 1996. Synthetic ion-exchange resins: soil and environmental studies. J. Environ. Qual. 25: 13-24.
  26. Sorokulova IB, Olsen EV, Chen IH, Fiebor B, Barbaree JM, Vodyanoy VJ, et al. 2005. Landscape phage probes for Salmonella typhimurium. J. Microbiol. Methods 63: 55-72. https://doi.org/10.1016/j.mimet.2005.02.019
  27. Strawn LK, Grohn YT, Warchocki S, Worobo RW, Bihn EA, Wiedmann M. 2013. Risk factors associated with Salmonella and Listeria monocytogenes contamination of produce fields. Appl. Environ. Microbiol. 79: 7618-7627. https://doi.org/10.1128/AEM.02831-13
  28. Su L, Jia W, Hou C, Lei Y. 2011. Microbial biosensors: A review. Biosens. Bioelectron. 26: 1788-1799. https://doi.org/10.1016/j.bios.2010.09.005
  29. Tovey ER, Bardo BA. 1989. Protein binding to nitrocellulose, nylon and PVDF membranes in immunoassays and electroblotting. J. Biochem. Biophys. Methods 19: 169-183. https://doi.org/10.1016/0165-022X(89)90024-9
  30. Velusamy V, Arshak K, Korostynska O, Oliwa K, Adley C. 2010. An overview of foodborne pathogen: in the perspective of biosensors. Biotechnol. Adv. 28: 232-254. https://doi.org/10.1016/j.biotechadv.2009.12.004

피인용 문헌

  1. Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor vol.132, pp.None, 2016, https://doi.org/10.3791/57208
  2. Studies on Lytic, Tailed Bacillus cereus-specific Phage for Use in a Ferromagnetoelastic Biosensor as a Novel Recognition Element vol.28, pp.1, 2016, https://doi.org/10.4014/jmb.1710.10033
  3. Nonconventional Therapeutics against Staphylococcus aureus vol.6, pp.6, 2018, https://doi.org/10.1128/microbiolspec.gpp3-0047-2018
  4. Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection vol.57, pp.2, 2019, https://doi.org/10.1007/s12275-019-8610-0
  5. Bacteriophages as Potential Tools for Detection and Control of Salmonella spp. in Food Systems vol.7, pp.11, 2019, https://doi.org/10.3390/microorganisms7110570
  6. Glass-Forming Ability of Fe-Ni Alloys Substituted by Group V and VI Transition Metals (V, Nb, Cr, Mo) Studied by Thermodynamic Modeling vol.51, pp.9, 2016, https://doi.org/10.1007/s11661-020-05897-9
  7. Strategies for Surface Immobilization of Whole Bacteriophages: A Review vol.7, pp.6, 2016, https://doi.org/10.1021/acsbiomaterials.1c00013
  8. Magnetoelastic Materials for Monitoring and Controlling Cells and Tissues vol.13, pp.24, 2021, https://doi.org/10.3390/su132413655