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Antioxidant capacity of anthocyanin-rich fruits and vegetables and changes of quality characteristics of black carrot added pudding according to storage

안토시아닌 함유 과채소류의 생리활성성분 함량과 항산화능 및 저장에 따른 자색당근 추출물 첨가 푸딩의 품질특성 비교

  • Kang, Suna (Department of Food and Nutrition, Institute of Basic Science, Hoseo University) ;
  • Lee, Soo Hyun (Department of Food and Nutrition, Institute of Basic Science, Hoseo University) ;
  • Shim, Young Nam (Department of Food and Nutrition, Institute of Basic Science, Hoseo University) ;
  • Oh, Min Ji (Department of Food and Nutrition, Institute of Basic Science, Hoseo University) ;
  • Lee, Na Ra (Department of Nanobiomechatronics, Hoseo University) ;
  • Park, Sunmin (Department of Food and Nutrition, Institute of Basic Science, Hoseo University)
  • Received : 2016.02.17
  • Accepted : 2016.05.11
  • Published : 2016.12.31

Abstract

We investigated the contents of total phenols, anthocyanins, carotenoids and the antioxidant capacity of black carrot, black currant, acaiberry, black raspberry, and blueberry. We also examined the physical and organoleptic characteristics of pudding with added black carrot extract following 7 days of storage. Black carrot and black raspberry had the highest total phenols. Blueberry contained the highest anthocyanins and black currant equaled black carrot in carotenoids. Anti-oxidant capacity measured by 1,1-diphenyl-1,2-picrylhydrazyl and 2,2-azino-bis-3-ethyl-benthiazolin-6-sulfonic acid was highest in black raspberry and black currant followed closely by black carrot. In pudding, redness was increased and blueness decreased by adding black carrot extract, but the trend was partially reversed during 7-day storage due to oxidation of the anthocyanins, which are stable in acid situation. As black carrot extract contents were increased, pH value decreased. The pudding's hardness, adhesiveness, springiness, cohesiveness, gumminess and chewiness were optimal with 7.5 % black carrot extract. In conclusion, black carrot is moderately rich in anthocyanins and phenolic compounds. In pudding adding 7.5 % black carrot extract was optimal for organoleptic qualities. However, additional studies are needed to develop methods for protecting anthocyanins from breaking-down during storage of back carrot pudding.

본 연구는 안토시아닌을 함유하는 자색당근, 블랙커런트, 아사이베리, 복분자, 블루베리의 생리활성 물질 및 항산화능을 측정하였고, 자색당근 농축액의 첨가량을 달리한 푸딩을 제조하여 7일간 푸딩의 품질을 측정하고 그 변화를 관찰하였다. 생리활성 물질인 phenol, carotenoid, flavonoid, anthocyanin 함량을 측정한 결과, 자색당근과 복분자는 폴리페놀을 다른 과일에 비해 높은 값을 나타내었는데, 안토시아닌함량은 블루베리, 카로티노이드 함량은 블랙커런트가 나머지 안토시아닌이 풍부한 과일과 채소에 비해 높았다. 그러나 DPPH와 ABTS로 측정한 항산화능은 복분자와 블랙커랜트가 다른 과일과 채소에 비해 높았고 자색당근과 블루베리가 중간 정도의 항산화능을 나타내었다. 그러므로 우리나라에서 재배가 용이하고 시장성이 좋은 자색당근 추출액을 첨가한 안토시아닌이 풍부한 푸딩을 제조하여 관능검사를 실행하고, 7일 동안 저장할 때의 물리적 특성을 조사하였다. 자색당근을 첨가량을 달리하여 푸딩을 제조하였을 때, pH는 자색당근 농축액의 첨가량이 증가할수록 낮아지는 경향을 보였고, 당도는 시간이 지나도 $24^{\circ}Bx$로 일정한 값을 나타내었다. 색도는 자색당근을 첨가량이 증가할수록 적색도가 증가하고 갈색도는 감소하였고 물성 검사에서는 경도가 낮고 탄성이 높았던 7.5 % 자색당근 추출액 첨가 푸딩이 가장 좋은 값을 나타내었다. 완성된 푸딩을 7일간 냉장 보관하여 품질 특성의 변화를 확인한 결과, 시간이 지남에 따라 적색도가 감소하였고 갈색도는 약간 증가하는 경향을 보였는데, 이는 시간이 흐름에 따라 자색당근이 산화되는 것과 관련이 있는 것으로 사료된다. 물성은 시간이 지남에 따라 큰 차이를 보이지 않았다. 결론적으로 7.5 %의 자색당근 추출액을 첨가한 푸딩이 관능적이나 물리적 특성이 적절하였다. 그러나 자색당근추출액에 함유된 안토시아닌의 산화 방지를 위해 pH와 온도를 낮게 유지하면서 푸딩을 제조하는 추가적인 연구가 필요할 것으로 사료된다.

Keywords

References

  1. Bagchi D, Sen CK, Bagchi M, Atalay M (2004) Anti-angiogenic, antioxidant, and anti-carcinogenic properties of a novel anthocyanin-rich berry extract formula. Biochem (Moscow) 69: 75-80 https://doi.org/10.1023/B:BIRY.0000016355.19999.93
  2. Blois MA (1958) Antioxdant determination by the use of a stable free radical. Nature 181: 1199-1200 https://doi.org/10.1038/1811199a0
  3. Celine MA, Dangles O, Amiot MJ (2001) Color stability of commercial anthocyanin-based extracts in relation to the phenolic composition. Protective effects by intra-and intermolecular copigmentation. J Agric & Food Chem 49: 170-176 https://doi.org/10.1021/jf000791o
  4. Choi SN, Kim HJ, Chung NY (2011) Nutrient intakes, nutritional knowledged, food habits, and lifestyle behaviors of obese children. J Kor Diet Assoc 17: 349-363
  5. Cristina AL, Barbara SH, Rachel LG, Olga J, Rosa MLR, James AJ (2005) Anthocyanins in aged blueberry-fed rats are found centrally and may enhance memory. Nutri Neurosci 8: 111-120 https://doi.org/10.1080/10284150500078117
  6. Flis S, Jastrzebski Z, Namiesnik J, Arancibia-Avila P, Toledo F, Leontowicz H, Leontowicz M, Suhaj M, Trakhtenberg S, Gorinstein S (2012) Evaluation of inhibition of cancer cell proliferation in vitro with different berries and correlation with their antioxidant levels by advanced analytical methods. J Pharm Biomed Anal 62: 68-78 https://doi.org/10.1016/j.jpba.2012.01.005
  7. Fukumoto LR, Mazza G (2000) Assessing antioxidant and prooxidant activities of phenolic compounds. J Agric Food Chem 48: 3597-3604 https://doi.org/10.1021/jf000220w
  8. Hakkinen S, Heinonen M, Karenlampi S, Mykkanen H, Ruuskanen J, Trrnen R (1999) Screening of selected flavonoids and phenolic acids in 19 berries. Food Res Int 32: 345-353 https://doi.org/10.1016/S0963-9969(99)00095-2
  9. Holton TA, Cornish EC (1995) Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell 7: 1071 https://doi.org/10.1105/tpc.7.7.1071
  10. Hwang SH, Ko SH (2010) Quality characteristics of muffins containing domestic blueberry (V.corymbosum). J East Asian Soc Dietary Life 20: 727-734
  11. Jia Z, Tang M, Wu J (1999) The determination of flavonoid contents in mulberry and their scarvenging effect on superoxide radicals. Food Chem 64: 555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
  12. Jong CH, Jang CW, Lee KY, Kim IH, Sim KH (2012) Chemical components and anti-oxidant activities of black currant. Kor J Food Presev 19: 263-270 https://doi.org/10.11002/kjfp.2012.19.2.263
  13. Kang J, Thakali KM, Xie C, Kondo M, Tong Y, Ou B, Jensenc G, Medinad MB, Schausse AG, Wu X (2012) Bioactivities of aa (Euterpe precatoria Mart.) fruit pulp, superior antioxidant and anti-inflammatory properties to Euterpe oleracea Mart. Food Chem 133: 671-677 https://doi.org/10.1016/j.foodchem.2012.01.048
  14. Kapasakalidis PG, Rastall RA, Gordon MH (2006) Extraction of polyphenols from processed black currant (Ribes nigrum L.) residues. J Agric Food Chem 54: 4016-4021 https://doi.org/10.1021/jf052999l
  15. Kaur C, Harish CK (2002) Anti-oxidant activity and total phenolic content of some Asian vegetables. Int J Food Sci Technol 37: 153-161 https://doi.org/10.1046/j.1365-2621.2002.00552.x
  16. Kirca A, Ozkan M, Cemeroglu B (2007) Effects of temperature, solid content and pH on the stability of black carrot anthocyanins. Food Chem 101: 212-218 https://doi.org/10.1016/j.foodchem.2006.01.019
  17. Lee JM, Robert WD, Ronald EW (2005) Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. J AOAC Int 88: 1269-1278
  18. Lee JN, Kim HE, Kim YS (2014) Anti-diabetic and anti-oxidative effects of Opuntia humijusa cladodes. J Kor Soc Food Sci Nutr 43: 661-667 https://doi.org/10.3746/jkfn.2014.43.5.661
  19. Marinova D, Ribarova F (2007) HPLC determination of carotenoids in Bulgarian berries. J Food Comp Anal 20: 370-374 https://doi.org/10.1016/j.jfca.2006.09.007
  20. Michale NC (2000) Anthocyanins - nature, occurrence and dietary burden. J Sci Food Agric 80: 2063-1072 https://doi.org/10.1002/1097-0010(200011)80:14<2063::AID-JSFA750>3.0.CO;2-2
  21. Mikkonen TP, Maatta KR, Hukkanen AT, Kokko HI, Torronen AR, Karenlampi SO, Karjalainen RO (2001) Flavonol content varies among black currant cultivars. J Agric Food Chem 49: 3274-3277 https://doi.org/10.1021/jf0010228
  22. Montilla EC, Arzaba MR, Hillebrand S, Winterhalter P (2011) Anthocyanin composition of black carrot (Daucus carota ssp. sativus var. atrorubens Alef.) cultivars Antonina, Beta Sweet, Deep Purple, and Purple Haze. J Agri Food Chem 59: 3385-3390 https://doi.org/10.1021/jf104724k
  23. Nho HJ, Jang SY, Park JJ, Yun HS, Park S (2013) Browning prevention of black carrot extract and the quality characteristics of jelly supplemented with black carrot extract. Kor J Food Culture 23: 293-302
  24. Nicolle C, Simon G, Rock E, Amouroux P, Remesy C (2004) Genetic variability influences carotenoid, vitamin, phenolic, and mineral content in white, yellow, purple, orange, and dark-orange carrot cultivars. J Am Soc Hort Sci 129: 523-529
  25. Nielsen IL, Haren GR, Magnussen EL, Dragsted LO, Rasmussen SE (2003) Quantification of anthocyanins in commercial black currant juices by simple high-performance liquid chromatography. Investigation of their pH stability and antioxidative potency. J Agri Food Chem 51: 5861-5866 https://doi.org/10.1021/jf034004+
  26. Novotny JA, Dueker SR, Zech LA, Clifford AJ (1995) Compartmental analysis of the dynamics of ${\beta}$-carotene metabolism in an adult volunteer. J Lipid Res 36: 1825-1838
  27. Octavio PL, Martha LCC, Monica VP, Talia HP (2010) Berries: improving human health and healthy aging, and promoting quality life-a review. Plant Food Human Nutri 65: 299-308 https://doi.org/10.1007/s11130-010-0177-1
  28. Park HM, Yang SJ, Kang EJ, Lee DH, Kim DI, Hong JH (2012) Quality characteristics and granule manufacture of mulberry and blueberry fruit extracts. Kor J Food Cookery Sci 28: 375-382 https://doi.org/10.9724/kfcs.2012.28.4.375
  29. Park SB, Kim SW, Kim YS, Na CS, Sim KS (2014) Effect of inclusion of Chitosan-Oligosaccahariede in drinking water on the blood component profile, immunity and antioxidative enzyme in broiler chickens. Korean J Organic Agric 22: 483-490 https://doi.org/10.11625/KJOA.2014.22.3.483
  30. Park SG, Song TH, Kim DH, Kim GH, Jang HI (2014) Quality properties of peach pudding added with Korean peach (Prunus persica L. Batsch) Juice and gelatin. J Kor Soc Food Sci Nutr 43: 265-272 https://doi.org/10.3746/jkfn.2014.43.2.265
  31. Puupponen-PR, Nohynek L, Meier C, Kahkonen M, Heinonen M, Hopia A, Oksman-Caldentey KM (2001) Antimicrobial properties of phenolic compounds from berries. J Appl Microbiol 90: 494-507 https://doi.org/10.1046/j.1365-2672.2001.01271.x
  32. Ra KS, Suh HJ, Chung SH, Son JY (1997) Antioxidant activity of sovent extract from onion skin. Kor J Food Sci Technol 29: 595-600
  33. Re R, Pellegrini N, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTs radical cation decolorization assay. Free Radical Biol Med 26: 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  34. Schauss AG, Wu X, Prior RL, Ou B, Patel D, Huang D, Kababick JP (2006) Phytochemical and nutrient composition of the freeze-dried amazonian palmberry, Euterpe oleraceae Mart. (acai). J Agric Food Chem 54: 8598-8603 https://doi.org/10.1021/jf060976g
  35. Seda E, Unal Y (2007) Microencapsulation of anthocyanin pigments of black carrot (Daucuscarota L.) by spray drier. J Food Engineer 80: 805-812 https://doi.org/10.1016/j.jfoodeng.2006.07.009
  36. Swain T, Hillis WE (1959) The phenolic constituents of Prunus domestica. I.-The quantitative analysis of phenolic constituents. J Sci Food Agric 10: 63-68 https://doi.org/10.1002/jsfa.2740100110
  37. Wallace TC, Giusti MM (2008) Determination of color, pigment, and phenolic stability in yogurt systems colored with nonacylated anthocyanins from Berberis boliviana L. as compared to other natural/synthetic colorants. J Food Sci 73: 241-248
  38. Wang SY, Lin HS (2000) Antioxidant activity in fruits and leaves of blackberry, raspberry and strawberries with culture and developmental stage. J Afri Food Chem 48: 140-146 https://doi.org/10.1021/jf9908345
  39. Xu ZS, Huang Y, Wang F, Song X, Wang GL, Xiong AS (2014) Transcript profiling of structural genes involved in cyanidin-based anthocyanin biosynthesis between purple and non-purple carrot (Daucus carota L.) cultivars reveals distinct patterns. BMC plant Biol 14: 262 https://doi.org/10.1186/s12870-014-0262-y
  40. Yu OK, Back HI, Cha YS (2008) Quality characteristics of pudding added with Bokbunja (Rubus coreanus Miquel) fruit juice and Bokbunja wine. J Kor Soc Food Culture 23: 616-620

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