Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Liquid-Liquid Extraction for Recovery of Paclitaxel from Plant Cell Cultures by Adding Inorganic Salts

식물세포배양으로부터 파클리탁셀 회수를 위한 무기염이 첨가된 액-액 추출

  • Ha, Geon-Soo (Department of Chemical Engineering, Kongju National University) ;
  • Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
  • 하건수 (공주대학교 화학공학부) ;
  • 김진현 (공주대학교 화학공학부)
  • Received : 2015.07.16
  • Accepted : 2015.09.08
  • Published : 2016.02.01
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Abstract

We developed a liquid-liquid extraction method using an inorganic salt to dramatically improve the recovery efficiency of the anticancer agent paclitaxel from plant cell cultures. As a result of liquid-liquid extraction using a diverse types of inorganic salt (NaCl, KCl, $K_2HPO_4$, $NaH_2PO_4$, $NaH_2PO_4{\cdot}2H_2O$), NaCl gave the highest yield (~96%) and lowest partition coefficient (0.053) of paclitaxel. The optimal NaCl/solvent ratio, methylene chloride/MeOH ratio, and pure paclitaxel content for liquid-liquid extraction using NaCl were 1% (w/v), 26% (v/v), and 0.066% (w/v), respectively. Under the optimal conditions developed in the present method, most of the paclitaxel (~96%) was recovered from biomass by a single extraction step. In addition, this method facilitated 3-fold higher recovery efficiency of paclitaxel in a shorter extraction number than the conventional liquid-liquid extraction method.

본 연구에서는 무기염을 첨가한 액-액 추출에 의해 식물세포인 바이오매스로부터 파클리탁셀 회수 방법을 획기적으로 개선하고자 하였다. 다양한 무기염(NaCl, KCl, $K_2HPO_4$, $NaH_2PO_4$, $NaH_2PO_4{\cdot}2H_2O$)을 이용하여 추출효율을 조사한 결과, NaCl에서 가장 낮은 분배계수(0.053)로 가장 높은 파클리탁셀 수율(~96%)을 얻을 수 있었다. NaCl을 이용한 액-액 추출에서 최적의 NaCl/용매 비와 메틸렌 클로라이드/메탄올 비는 각각 1%(w/v)와 26%(v/v)이었다. 또한 최적의 NaCl/용매 비와 메틸렌 클로라이드/메탄올 비에서 파클리탁셀 함량에 따른 영향을 조사한 결과, 순수 파클리탁셀 함량 0.066%(w/v)에서 가장 낮은 분배계수(0.053)로 가장 높은 수율(~96%)을 얻을 수 있었다. 기존 액-액 추출의 경우 총 3회의 추출로 파클리탁셀을 95% 정도 회수 가능한 반면 무기염을 이용한 방법의 경우 단 1회 추출로 대부분의 파클리탁셀을 회수(~96%) 가능하였다.

Keywords

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