Korean Chemical Engineering Research

  • 제54권1호
  • /
  • Pages.52-56
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  • 2016
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  • 0304-128X(pISSN)
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  • 2233-9558(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
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피페리딘계 이온성 액체와 포스페이트 염으로 구성된 수상이성분계를 이용한 숙신산의 추출

Liquid Extraction of Succinic Acid by Aqueous Two-Phase Systems Composed of Piperidinium Ionic Liquids and Phosphate Salt

  • 이우윤 (한국교통대학교 화공생물공학과) ;
  • 홍연기 (한국교통대학교 화공생물공학과)
  • Lee, Woo Yun (Department of Chemical and Biological Engineering, Korea National University of Transportation) ;
  • Hong, Yeon Ki (Department of Chemical and Biological Engineering, Korea National University of Transportation)
  • 투고 : 2015.04.21
  • 심사 : 2015.05.19
  • 발행 : 2016.02.01
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초록

본 연구에서는 숙신산 추출을 위한 효과적인 분리공정으로서 피페리딘계 이온성 액체/$K_2HPO_4$에 의한 수상이성분계를 적용하여 수상이성분계 형성 특성 및 추출특성을 고찰하였다. 실험 결과 $K_2HPO_4$ 수용액에 적정량의 피페리딘계 이온성 액체를 첨가함에 따라 안정한 수상이성분계가 형성됨을 확인할 수 있었으며 이성분계 형성능력은 이온성 액체 내 양이온의 알킬 사슬길이에 따라 증가하였다. $K_2HPO_4$ 수용액을 기준으로 피페리딘계 이온성 액체의 수상이성분계 형성 능력은 이미다졸계나 피롤리딘계에 비해 우수하였다. 피페리딘계 이온성 액체를 이용한 수상이성분계 추출에 있어 숙신산 추출 효율은 75~95%의 범위를 가지며 이 값들은 이미다졸계나 피롤리딘계와 큰 차이가 없었다. 그러므로 피페리딘계 이온성 액체를 이용한 수상이성분계를 숙신산 추출에 적용할 경우 이미다졸계 및 피롤리딘계에 비해 적은 양의 이온성 액체를 사용하여 높은 추출효율을 얻을 수 있음을 확인할 수 있었다.

As an effective method for extraction of succinic acid, aqueous two-phase systems based on piperidinium ionic liquids were used in this study. Effects of the alkyl chain length of cation in piperinidium ionic liquids on phase diagram and extraction efficiencies were investigated. Experimental results show that aqueous two phase systems can be formed by adding appropriate amount of piperidinium ionic liquids to aqueous $K_2HPO_4$ solutions. It can be found that the ability of piperidinium ionic liquids for phase separation followed the order [OMPip][Br]>[HMPip][Br]>[BMPip][Br]>[EMPip][Br]. The biphase-forming ability of piperidinium ionic liquids was higher than that of imidazolium and pyrrolidinium ionic liquids in the presence of $K_2HPO_4$. 75~95% of the succinic acid could be extracted into the ionic liquid-rich phase in a single-step extraction. There was little difference in the extraction efficiency of succinic acid by piperidinium ionic liquids comparing to other ionic liquids such as imidazolium and pyrrolonidium ionic liquids. This aqueous two phase system by piperidinium ionic liquid is suggested to have effective application for the separation of succinic acid.

키워드

참고문헌

  1. Sauer, M., Porro, D., Mattanovich, D. and Branduardi, P., "Microbial Production of Organic Acids: Expanding the Markets," Trends Biotechnol., 26, 100-108(2008). https://doi.org/10.1016/j.tibtech.2007.11.006
  2. Okino, S., Noburyu, R., Suda, M., Jojima, T., Inui, M. and Yukawa, H., "An Efficient Succinic Acid Production Process in a Metabolically Engineered Corynebacterium Glutamicum Strain," Appl. Microbiol. Biotechnol., 81, 459-264(2008). https://doi.org/10.1007/s00253-008-1668-y
  3. Kurzrock, T. and Weuster-Botz, D., "Recovery of Succinic Acid from Fermentation Broth," Biotechnol. Lett., 32, 331-339(2010). https://doi.org/10.1007/s10529-009-0163-6
  4. Cheng, K. K., Zhao, X. B., Zeng, J., Wu, R. C., Xu, Y. Z., Liu, D. H. and Zhang, J. A., "Downstream Processing of Biotechnological Produced Succinic Acid," Appl. Microbiol. Biotechnol., 139, 841-850(2012).
  5. Gu, B. H., Zheng, P., Yan, Q. and Liu, W., "Aqueous Two-Phase System: An Alternative Process for Recovery of Succinic Acid from Fermentation Broth," Sep. Purif. Technol., 138, 47-54(2014). https://doi.org/10.1016/j.seppur.2014.09.034
  6. Martinez-Aragon, M., Burghoff, S., Goetheer, E. L. V. and de Haan, A. B., "Guidelines for Solvent Selection for Carrier Mediated Extraction," Sep. Purif. Technol., 65, 65-72(2009). https://doi.org/10.1016/j.seppur.2008.01.028
  7. Desai, R. K., Streefland, M., Wijiffels, R. H. and Eppink, M. H. M., "Extraction and Stability of Selected Proteins in Ionic Liquid Based Aqueous Two Phase Systems," Green Chem., 16, 2670-2679(2014). https://doi.org/10.1039/C3GC42631A
  8. Zafarani-Moattar, M. T. and Hamzehzadeh, S., "Partitioning of Amino Acids in the Aqueous Biphasic System Containing the Water-Miscible Ionic Liquid 1-Butyl-3-Methylimidazolium Bromide and the Water-Structuring Salt Potassium Citrate," Biotechnol. Prog., 27, 986-997(2011). https://doi.org/10.1002/btpr.613
  9. Gotowski, K. E., Broker, G. A., Willauer, H. D., Huddleston, J. G., Swatloski, R. P., Holbrey, J. D. and Rogers, R. D., "Controlling the Aqueous Miscibility of Ionic Liquids: Aqueous Biphasic Systems of Water-Miscible Ionic Liquids and Water-Structuring Salts for Recycle, Metathesis, and Separations," J. Am. Chem. Soc., 125, 6632-6633(2003). https://doi.org/10.1021/ja0351802
  10. Pei, Y., Wang, J., Wu, K., Xuan, X. and Lu, X., "Ionic Liquid-Based Aqueous Two-Phase Extraction of Selected Proteins," Sep. Purif. Technol., 64, 288-295(2009). https://doi.org/10.1016/j.seppur.2008.10.010
  11. Claudio, A. F. M., Freire, M. G., Freire, C. S. R., Silvestre, A. J. D. and Coutinho, J. A. P., "Extraction of Vanillin Using Ionic-Liquid-Based Aqueous Two-Phase Systems," Sep. Purif. Technol., 75, 39-47(2010). https://doi.org/10.1016/j.seppur.2010.07.007
  12. Lee, Y. H., Kang, J, W., Hong, Y. K. and Kim, K.-S., "Extraction Equilibria of Succinic Acid by Using Aqueous Two Phases System Containing Imidazolium Ionic Liquids and Salts," Clean Technol., 20, 349-353(2014). https://doi.org/10.7464/ksct.2014.20.4.349
  13. Lee, Y. H., Lee, W. Y., Kim, K.-S. and Hong, Y. K., "Extraction Equilibrium of Acrylic Acid by Aqueous Two-Phase Systems Using Hydrophilic Ionic Liquids," Korean Chem. Eng. Res., 52, 627-631(2014). https://doi.org/10.9713/kcer.2014.52.5.627
  14. Hong, Y. K., "Effect of Alcohols and Carboxylic Acids on Extraction Characteristics for 1,3-Propanediol by Aqueous Two Phases Systems," Korean Chem. Eng. Res., 51, 575-579(2013). https://doi.org/10.9713/kcer.2013.51.5.575
  15. Merchuk, J. C., Andrews, B. A. and Asenjo, J. A., "Aqueous Two-Phase Systems for Protein Separation: Studies on Phase Inversion," J. Chromatogr. B., 711, 285-293(1998). https://doi.org/10.1016/S0378-4347(97)00594-X
  16. Neves, C. M. S. S., Ventura, S. P. M., Freire, M. G., Marrocho, I. M. and Coutinho, J. A. P., "Evaluation of Cation Influence on the Formation and Extraction Capability of Ionic-Liquid-Based Aqueous Biphasic Systems," J. Phys. Chem. B., 113, 5194-5199(2009). https://doi.org/10.1021/jp900293v

피인용 문헌

  1. Ionic liquid-based salting-out extraction of bio-chemicals vol.30, pp.None, 2021, https://doi.org/10.1016/j.cjche.2020.11.004