Journal of Digital Convergence (디지털융복합연구)

The Society of Digital Policy and Management (한국디지털정책학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-Inflammatory Effects of Fusion-Fermented Aralia continentalis Radix (fACR) on THP-1 cells

THP-1 세포에서 융합 발효 독활의 항염증 효과

  • Jung, Young-Mi (Dept. of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Dong-Sub (Dept. of Health Care & Biotechnology, Kyungwoon University) ;
  • Kwon, Ki-Sang (Dept. of Biomedical Laboratory Science, Kyungwoon University)
  • 정영미 (경북대학교 유전공학과) ;
  • 이동섭 (경운대학교 보건바이오학과) ;
  • 권기상 (경운대학교 임상병리학과)
  • Received : 2015.11.24
  • Accepted : 2016.01.20
  • Published : 2016.01.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study was to demonstrate the anti-inflammation effect using extracts derived from fusion-fermentation to add the function of Araliae Continentalis Radix (fACR). Since there are limitations to the use of ACR, available strains were selected through fermentation using lactobacillus strains, and the increases in total amount of polyphenols and amino acids was confirmed through comparison with Hot Water Extract of ACR. To determine the anti-inflammatory effect of the fACR was measure cytokine inflammation associated with arthritis, the arthritis when cartilage destruction is accompanied mainly MMP-9 activity was confirmed to evaluate the inhibition effect. These results show that fermentation using lactobacillus increases major biological activities and inflammatory response-restraining effects of ACR. This study is expected to be a basis for studying the preventive effect of fACR on arthritis.

본 연구는 독활의 기능을 부가시키고자 발효과정을 통해 융합-발효 추물물을 얻어 항염증효과를 확인 하고자 한다. 독활을 이용하는데 한계가 있어 유산균주들을 활용한 발효과정을 통해 이용 가능한 균주를 선별하고, 독활의 열수 추출물과 비교하여 총 폴리페놀, 아미노산 총량, 주요 생리활성 미네랄 성분 분석 결과 발효과정을 통해 증가함을 확인하였다. 독활의 발효추출물의 항염증 효과를 확인하고자 관절염 염증반응과 관련된 사이토카인들을 측정하였고, 관절염시 주로 동반되는 연골조직 파괴를 억제 효능을 평가하기 위해 MMP-9 활성도를 확인하였다. 이러한 결과들은 유산균을 이용한 발효과정을 통해 독활의 주요 생리활성이 증가하고 염증반응 억제 효과도 증가하는 것으로 보여진다. 이 연구는 독활의 관절염 예방적 효과를 연구하는데 있어 기초 연구가 될 것으로 기대된다.

Keywords

References

  1. N. H. Dang, X. F. Zhang, M. S. Zheng, K. H. Son, H. W. Chang, H. P. Kim, K. H. Bae, S. S. Kang, " Inhibitory constituents against cyclooxygenases from Aralia cordata Thunb", Arch Pharm Res, Vol. 28, pp. 28-33, 2005. https://doi.org/10.1007/BF02975131
  2. W. S. Han, "Isolation of the antimicrobial compounds from Aralia cordata Thunb. extract", Koren J, Medicinal Crop Sci Vol. 13, pp. 182-185, 2005.
  3. S. I. Jeong, W. S. Han, Y. H. Yun, K. J. Kim, "Continentalic acid from Aralia continentalis shows activity against methicillin-resistant Staphylococcus aureus", Phytother Res, Vol. 20, pp. 511-514, 2006. https://doi.org/10.1002/ptr.1894
  4. H. J. Park, M. S. Hong, J. S. Lee, K. H. Leem, C. J. Kim,J. W. Kim and S. Lim, "Effects of Aralia continentalis on hyperalgesia with peripheral inflammation", Phytother Res Vol. 19, pp. 511-513, 2005. https://doi.org/10.1002/ptr.1693
  5. B. S. Jeon, J. W. Park, B. K. Kim, H. K. Kim, T. S. Jung, J. R. Hahm, D. R. Kim, Y. S. Cho, J. Y. Cha, "Fermented mushroom milk supplemented dietary fiber prevents the onset of obesity and hypertriglyceridemia in Otsuka Long-Evans Tokushima Fatty (OLETF) rats", Diabetes Obes Metab, Vol. 7, pp. 709-715, 2005. https://doi.org/10.1111/j.1463-1326.2005.00456.x
  6. H. T. Trinh, S. J. Han, S. W. Kim, Y. C. Lee, D. H. Kim, "Bifidus fermentation increases hypolipidemic and hypoglycemic effects of red ginseng", J Microbiol Biotechnol, Vol. 17, No. 7, pp. 1127-1133, 2007.
  7. S. I. Lee, J. G. Shin, S. D. Kim, "Effect of ginsengchungkukjang effects on lipid profiles of serum in alcohol administered diabetic-induced rats", J. Korean Soc Food Sci Nutr, Vol. 34, pp. 1362-1366, 2005. https://doi.org/10.3746/jkfn.2005.34.9.1362
  8. S. Y. Kim, S. I. Jeong, S. Z. Kim, J. S. Shim, S. I. Jang, "Effects of Continentalic from Aralia Continentalis on Growth Inhibition and Apoptosis Induction in Human Leukemia HL-60 Cells", Korean J Oriental Physiol & Pathol, Vol. 23, No. 6, pp. 1314-1319, 2009.
  9. L. M. Baek, L. Y. Park, K. S. Park, S. H. Lee, "Effect of Starter Cultures on the Fermentative Characteristics of Cheonggukjang", Korean J Food Sci Technol, Vol. 40, No. 4, pp. 400-405, 2008.
  10. E. H. Han, T. S. Lee, B. S. Noh, D. S. Lee, "Volatile flavor components in mash of takju prepared by using different nuruks", Korean J Food Sci Technol, Vol. 29, pp. 563-570, 1997.
  11. H. R. Park, S. J. Hong, "Research on Natural Medicine for Wellness and Oral Health", J Digital Convergence Vol.13, No.5, pp.357-363, 2015.
  12. Y. H. Pyo, "Effect of Monascus-fermentation on the content of GABA and free amino acids in soybean", J Korean Soc Food Sci Nutr, Vol. 37, pp. 1208-1213, 2008. https://doi.org/10.3746/jkfn.2008.37.9.1208
  13. J. H. Lee, J. S. Park, "Antioxidant Activities of Rice Bran Extracts for Wellness Convergence", J Digital Convergence, Vol.13, No. 2 pp.401-406, 2015. https://doi.org/10.14400/JDC.2015.13.2.401
  14. M. L. Buckley, D. P. Ramji, "The influence of dysfunctional signaling and lipid homeostasis in mediating the inflammatory responses during atherosclerosis", Biochim Biophys Acta, Vol. 1852, No. 7, pp.1498-1510, 2015. https://doi.org/10.1016/j.bbadis.2015.04.011
  15. Michael Schuliga, "NF-kappaB Signaling in Chronic Inflammatory Airway Disease", Biomolecules, Vol. 5, No. 3, pp. 1266-1283, 2015. https://doi.org/10.3390/biom5031266
  16. C. Y. Wenham, M. McDermott, P. G. Conaghan, "Biological Therapies in Osteoarthritis", Curr Pharm Des, Vol. 21, No. 17, pp. 2206-2215, 2015. https://doi.org/10.2174/1381612821666150310144940
  17. G. P. Amorino, R. L. Hoover, "Interactions of monocytic cells with human endothelial cells stimulate monocytic metalloproteinase production", Am J Pathol, Vol. 152, pp. 199-207, 1998.
  18. C. N. Andrew, "Metalloproteinases expression in monocytes and macrophages and its relationship to atherosclerotic plaque instability", Arterioscler Thromb Vasc Biol, Vol. 28, pp. 2108 -2114, 2008. https://doi.org/10.1161/ATVBAHA.108.173898
  19. A. Bar-Or, R. K. Nuttall, M. Duddy, A. Alter, H. J. Kim, I. Ifergan, C. J. Pennington, P. Bourgoin, D. R. Edwards, V. W. Yong, "Analyses of all matrix metalloproteinase members in leukocytes emphasize monocytes as major inflammatory mediators in multiple sclerosis", Brain, Vol. 126, pp. 2738-2749, 2003. https://doi.org/10.1093/brain/awg285
  20. J. Lim, Y. Cho, D. H. Lee, B. H. Jung, H. S. Kang, T. J. Kim, K. J. Rhee, T. U. Kim, Y. S. Kim, "Upregulation of MMP is Mediated by MEK1 Activation During Differentiation of Monocyte into Macrophage", Exp Biomed Sci, Vol. 18, No. 2, pp. 104-111, 2012.

Cited by

  1. Mouse Single Oral Dose Toxicity Test of Lactobacillus-fermented Araliae Continentalis Radix Aqueous Extracts (fACR) vol.26, pp.2, 2016, https://doi.org/10.5352/JLS.2016.26.2.204