Journal of Digital Convergence (디지털융복합연구)

The Society of Digital Policy and Management (한국디지털정책학회)
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Optimization of enzymatic hydrolysis of viscera waste proteins of black body fowl(Yeonsan Ogae) to produce peptides using a commercial protease and it's characters analysis

단백질 분해효소를 이용한 연산오계 내장 펩타이드 생산 최적화 및 특성분석

  • Choi, So-Young (Department of Food Science and Biotechnology, Joongbu University) ;
  • Kim, A-Yeon (Department of Food Science and Biotechnology, Joongbu University) ;
  • Song, Yu-Rim (Department of Food Science and Biotechnology, Joongbu University) ;
  • Ji, Joong-Gu (Department of Oriental Health Care, Joongbu University) ;
  • Yoo, Sun-Kyun (Department of Food Science and Biotechnology, Joongbu University)
  • 최소영 (중부대학교 식품생명과학과) ;
  • 김아연 (중부대학교 식품생명과학과) ;
  • 송유림 (중부대학교 식품생명과학과) ;
  • 지중구 (중부대학교 한방건강관리학과) ;
  • 유선균 (중부대학교 식품생명과학과)
  • Received : 2015.11.12
  • Accepted : 2016.01.20
  • Published : 2016.01.28
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Abstract

Yeonsan Ogae has been known as supporting health and high efficacy of treatment. In recent days, as the efficacy of functional peptides has known, the optimization of oligo peptides production and its characteristics from Ogae viscera has been performed. Response surface method was used to perform the optimizaion of enzyme hydrolysis. The range of processes was temperature (40, 50 and $60^{\circ}C$), pH(6.0, 7.0 and 8.0), and enzyme(1, 2 and 3%). The degree of hydrolysis, amono acids, molecular weight of products were analyzed. The optimum process of enzyme hydrolysis were determined as temperature $58^{\circ}C$, pH 7.5, and enzyme concetration 3%. At optimum conditions, the degree of hydrolysis after 2 h reaction was 75-80%. The total amino acids of amino acid and were 386.15 mg/100 g and 155.26 mg/100 g, respectively. The molecular weight of products by using Maldi-TOF was ranged from 300 to 1,000 Da.

연산오계는 오래전부터 건강기능 증진 및 치료 효능이 높은 것으로 알려져 왔다. 최근 건강 기능식품 소재로 기능성 펩타이드 효능이 알려짐에 따라, 연산오계 내장 부산물로 부터 올리고 펩타이드 최적 생산 공정 및 생성물 특성에 대하여 연구를 수행하였다. 최적 효소가수 분해 공정 표면반응 분석을 이용하여 수행하였다. 최적 공정 조건을 확립하기 위해서 온도 (40, 50, $60^{\circ}C$), pH (pH 6.0, 7.0, 8.0), 효소 (1, 2, 3%) 범위에서 수행을 하였다. 생성물에 대한 가수분해도, 아미노산, 분자량 분포를 분석하였다. 효소 가수분해 최적 온도는 $58^{\circ}C$, pH 7.5, 효소의 농도는 3% 이었다. 최적 조건에서 2 시간 효소 가수분해를 한 결과 75-80% 이었다. 구성 아미노산의 총 함량은 386.15 mg/100 g 이었고 유리 아미노산 총량은 155.26 mg/100 g. 분자량를 Maldi-TOF 으로 분석을 한 결과 90% 이상이 300-1,000 Da 분포를 보여주었다.

Keywords

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