Journal of Korean Biological Nursing Science

Korean society of Biological Nursing Science (한국기초간호학회)
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High-fat Intake is Associated with Alteration of Peripheral Circadian Clock Gene Expression

고지방식이에 의한 말초 생체시계 유전자 발현 변화

  • Park, Hyun-Ki (Biobehavioral Center, Mo-Im Kim Nursing Research Institute, Yonsei University) ;
  • Park, Jae-Yeo (Biobehavioral Center, Mo-Im Kim Nursing Research Institute, Yonsei University) ;
  • Lee, Hyangkyu (Department of Clinical Nursing, College of Nursing, Yonsei University)
  • 박현기 (연세대학교 간호대학 김모임연구소 생행동연구센터) ;
  • 박재여 (연세대학교 간호대학 김모임연구소 생행동연구센터) ;
  • 이향규 (연세대학교 간호대학 김모임연구소 생행동연구센터)
  • Received : 2016.11.03
  • Accepted : 2016.11.20
  • Published : 2016.11.30
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Abstract

Purpose: Recent studies demonstrated disruption of the circadian clock gene is associated with the development of obesity and metabolic syndrome. Obesity is often caused by the high calorie intake, In addition, the chronic stress tends to contribute to the increased risk for obesity. To evaluate the molecular mechanisms, we examined the expression of circadian clock genes in high fat diet-induced mice models with the chronic stress. Methods: C57BL/6J mice were fed with a 45% or 60% high fat diet for 8 weeks. Daily immobilization stress was applied to mice fed with a 45% high fat for 16 weeks. We compared body weight, food consumption, hormone levels and metabolic variables in blood. mRNA expression levels of metabolic and circadian clock genes in both fat and liver were determined by quantitative RT-PCR. Results: The higher fat content induced more severe hyperglycemia, hyperlipidemia and hyperinsulinemia, and these results correlated with their relevant gene expressions in fat and liver tissues. Chronic stress had only minimal effects on metabolic variables, but it altered the expression patterns of metabolic and circadian clock genes. Conclusion: These results suggest that the fat metabolism regulates the function of the circadian clock genes in peripheral tissues, and stress hormones may contribute to its regulation.

Keywords

References

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