International Journal of Industrial Entomology and Biomaterials

  • 제33권2호
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  • Pages.113-120
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  • 2016
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
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Comparison of gloverin gene expression patterns between domesticated and wild silkworms

  • Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Choi, Kwang-Ho (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Kim, Sung-Wan (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Park, Seung-Won (Department of Biotechnology, Catholic University of Daegu)
  • 투고 : 2016.11.08
  • 심사 : 2016.11.11
  • 발행 : 2016.12.31
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초록

Bombyx mandarina is widely accepted as ancestor of B. mori. Silkworms are served as well-characterized models for understanding the mechanism for the genetic regulation of development. In this study, we performed RNA-Seq analysis to examine tissue-expression of gloverin isoforms of the silk-gland, mid-gut, and fat body in B. mandarina. BLAST analysis revealed that four gloverin isoform gene sequences of B. mandarina were highly similar to B. mori. To identify the difference between two species, the expression profile of gloverin was measured by semi- RT-PCR analysis. The specific expression of gloverin isoform genes was observed mainly in the fat body from B. mori but not B. mandarina. However, all of tissues in the wild-type silkworm could induce the upregulation of compared with the B. mori. To validate the sudden increase in gloverin gene expression in the mid-gut tissue of B. mandarina, we were using qRT-PCR. Relative mRNA expression rate of gloverin at the wild-type silkworm was much higher than domestic silkworm. Comparative genomics between domesticated and wild silkworms showed different tissue-expression levels in some of immune related genes. These results are suggesting a trend toward decreasing immunity related genes expression during domestication. Further studies are needed to elucidate the silkworm domestication and an invaluable resource for wild silkworm genomics research.

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참고문헌

  1. Axen A, Carlsson A, Engstrom A, Bennich H (1997) Gloverin, an antibacterial protein from the immune hemolymph of Hyalophora pupae. Eur J Biochem 247, 614-619. https://doi.org/10.1111/j.1432-1033.1997.00614.x
  2. Bulet P, Hetru C, Dimarcq JL, Hoffmann D (1999) Antimicrobial peptides in insects: structure and function. Dev Comp Immunol 23, 329-344. https://doi.org/10.1016/S0145-305X(99)00015-4
  3. Castillo JC, Creasy T, Kumari P, Shetty A, Shokal U, Tallon LJ (2015) Drosophila anti-nematode and antibacterial immune regulators revealed by RNA-Seq. BMC Genomics 16, 519-528. https://doi.org/10.1186/s12864-015-1690-2
  4. Cheng T, Fu B, Wu Y, Long R, Liu C, Xia Q (2015) Transcriptome sequencing and positive selected genes analysis of Bombyx mandarina. PLOS ONE 10, e0122837. https://doi.org/10.1371/journal.pone.0122837
  5. Costa V, Aprile M, Esposito R, Ciccodicola A (2013) RNA-Seq and human complex diseases: recent accomplishments and future perspectives. Eur J Hum Genet 21, 134-142. https://doi.org/10.1038/ejhg.2012.129
  6. Daines B, Wang H, Wang L, Li Y, Han Y, Emmert D, Gelbart W, Wang X, Li W, Gibbs R, Chen R (2011) The Drosophila melanogaster transcriptome by paired-end RNA sequencing. Genome Res 21, 315-324. https://doi.org/10.1101/gr.107854.110
  7. de Klerk E, den Dunnen JT, 't Hoen PA (2014) RNA sequencing: from tag-based profiling to resolving complete transcript structure. Cell Mol Life Sci 71, 3537-3551. https://doi.org/10.1007/s00018-014-1637-9
  8. Ekblom R, Galindo J (2011) Applications of next generation sequencing in molecular ecology of non-model organisms. Heredity 107, 1-15. https://doi.org/10.1038/hdy.2010.152
  9. Hara S, Yamakawa M (1995a) Moricin, a novel antibacterial peptide, isolated from the silkworm, Bombyx mori. J Biol Chem 270, 29923-29927. https://doi.org/10.1074/jbc.270.50.29923
  10. Hara S, Yamakawa M (1995b) A novel antibacterial peptide family isolated from the silkworm, Bombyx mori. Biochem J 310, 651-656. https://doi.org/10.1042/bj3100651
  11. Hoffman JA, Kafatos FC, Janeway CA, Ezekowitz RA (1999) Phylogenetic perspectives in innate immunity. Science 284, 1313-1318. https://doi.org/10.1126/science.284.5418.1313
  12. Hoffmann JA (2003) The immune response of Drosophila. Nature 426, 33-38. https://doi.org/10.1038/nature02021
  13. Huang L, Cheng T, Xu P, Cheng D, Fang T, Xia Q (2009) A genome-wide survey for host response of Silkworm, Bombyx mori during pathogen bacillus bombyseptieus infection. PLos ONE 4, e8098. https://doi.org/10.1371/journal.pone.0008098
  14. Hultmark D (2003) Drosophila immunity: paths and patterns. Curr Opin Immunol 15, 12-19. https://doi.org/10.1016/S0952-7915(02)00005-5
  15. Kaneko Y, Furukawa Y, Tanaka H, Yamakawa M (2007) Expression of Antimicrobial Peptide Genes Encoding Enbocin and Gloverin Isoforms in the Silkworm, Bombyx mori. Biosci Biotechnol Biochem 71, 2233-2241. https://doi.org/10.1271/bbb.70212
  16. Kaneko Y, Furukawa S, Tanaka H, Yamakawa M (2007) Expression of antimicrobial peptide genes encoding Enbocin and Gloverin isoforms in the silkworm, Bombyx mori. Biosci Biotechnol Biochem 71, 2233-2241. https://doi.org/10.1271/bbb.70212
  17. Kawaoka S, Katsuma S, Daimon T, Isono R, Omuro N, Mita K, Shimada T (2008) Functional analysis of four Gloverin-like genes in the silkworm, Bombyx mori. Arch Insect Biochem Physiol 67, 87-96. https://doi.org/10.1002/arch.20223
  18. Kurata S, Ariki S, Kawabata S (2006) Recognition of pathogens and activation of immune responses in Drosophila and horseshoe crab innate immunity. Immunobiology 211, 237-249. https://doi.org/10.1016/j.imbio.2005.10.016
  19. Lemaitre B (2004) The road to Toll. Nat Rev Immunol 4, 521-527. https://doi.org/10.1038/nri1390
  20. Lemaitre B, Hoffmann J (2007) The host defense of Drosophila melanogaster. Annu Rev Immunol 25, 697-743. https://doi.org/10.1146/annurev.immunol.25.022106.141615
  21. Liu T, Tang S, Zhu S, Tang Q, Zheng X (2014) Transcriptome comparison reveals the patterns of selection in domesticated and wild ramie (Boehmeria nivea L. Gaud). Plant Mol Biol 86, 85-92. https://doi.org/10.1007/s11103-014-0214-9
  22. Mrinal N, Nagaraju J (2008) Intron loss is associated with gain of function in the evolution of the gloverin family of antibacterial genes in Bombyx mori. J Biol Chem 283, 23376-23387. https://doi.org/10.1074/jbc.M801080200
  23. Ozsolak F, Milos PM (2011) RNA sequencing: advances, challenges and opportunities. Nat Rev Genet 12, 87-98. https://doi.org/10.1038/nrg2934
  24. Park SW, Kang SW, Goo TW, Kim SR, Lee GG, Paik SY (2010) Tissue-specific gene expression analysis of silkworm (Bombyx mori) by quantitative real-time RT-PCR. BMB Rep 43, 480-484. https://doi.org/10.5483/BMBRep.2010.43.7.480
  25. Szovenyi P, Perroud PF, Symeonidi A, Stevenson S, Quatrano RS, Rensing SA et al. (2015) De novo assembly and comparative analysis of the Ceratodon purpureus transcriptome. Mol Ecol Resour 15, 203-215. https://doi.org/10.1111/1755-0998.12284
  26. Tanaka H, Ishibashi J, Fujita K, Nakajima Y, Sagisaka A et al. (2008) A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori. Insect Biochem Mol Biol 38, 1087-1110. https://doi.org/10.1016/j.ibmb.2008.09.001
  27. Wang Z, Gerstein M, Snyder M (2009) RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet 10, 57-63. https://doi.org/10.1038/nrg2484
  28. Weinstock GM, Robinso GE et al. (2006) Insights into social insects from the genome of the honeybee Apis mellifera. Nature 443, 931-949. https://doi.org/10.1038/nature05260
  29. Xia Q, Zhou Z, Lu C, Cheng D, Dai F et al. (2004) A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science 306, 1937-1940. https://doi.org/10.1126/science.1102210
  30. Xia Q, Cheng D, Duan J, Wang G, Cheng T, Zha X, Liu C et al. (2007) Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori. Genome Biol 8, R162. https://doi.org/10.1186/gb-2007-8-8-r162
  31. Xiang H, Li X, Dai F, Xu X, Tan A, Chen L et al. (2013) Comparative methylomics between domesticated and wild silkworms implies possible epigenetic influences on silkworm domestication. BMC genomics 14, 646. https://doi.org/10.1186/1471-2164-14-646
  32. Xuan J, Yu Y, Qing T, Guo L, Shi L (2013) Next-generation sequencing in the clinic: promises and challenges. Cancer Lett 340, 284-295. https://doi.org/10.1016/j.canlet.2012.11.025
  33. Yang J, Wang X, Tang S, Shen Z, Wu J (2015) Peptidoglycan Recognition Protein S2 From Silkworm Integument: Characterization, Microbe-Induced Expression, and Involvement in the Immune-Deficiency Pathway. J Insect Sci 15.
  34. Zhao Q, Han MJ, Sun W, Zhang Z (2014) Copy number variations among silkworms. BMC genomics 15, 251-259. https://doi.org/10.1186/1471-2164-15-251