한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제19권3호
  • /
  • Pages.135-144
  • /
  • 2015
  • /
  • 2465-9525(pISSN)
  • /
  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지
DOI QR코드

DOI QR Code

Four Members of Heat Shock Protein 70 Family in Korean Rose Bitterling (Rhodeus uyekii)

  • Kim, Jung Hyun (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Dong, Chun Mae (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Julan (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • An, Cheul Min (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Baek, Hae Ja (Dept. of Marine Biology, Graduate School, Pukyong National University) ;
  • Kong, Hee Jeong (Biotechnology Research Division, National Fisheries Research and Development Institute)
  • 투고 : 2015.06.17
  • 심사 : 2015.07.07
  • 발행 : 2015.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Heat shock protein (HSP) 70, the highly conserved stress protein families, plays important roles in protecting cells against heat and other stresses in most animal species. In the present study, we identified and characterized four Hsp70 (RuHSP4, RuHSC70, RuHSP12A, RuGRP78) family proteins based on the expressed sequence tag (EST) analysis of the Korean rose bitterling R. uyekii cDNA library. The deduced RuHSP70 family has high amino acid identities of 72-99% with those of other species. Phylogenetic analysis revealed that RuHsp70 family clustered with fish groups (HSP4, HSC70, HSP12A, GRP78) proteins. Quantitative RT-PCR analysis showed the specific expression patterns of RuHsp70 family members in the early developmental stages and several tissues in Korean rose bitterling. The expression of 4 groups of Hsp70 family was detected in all tested tissue. Particularly, Hsp70 family of Korean rose bitterling is highly expressed in hepatopancreas and sexual gonad (testis and ovary). The expression of Hsp70 family was differentially regulated in accordance with early development stage of Rhodeus uyekii.

키워드

참고문헌

  1. Arai A, Naruse K, Mitani H, Shima A (1995) Cloning and characterization of cDNAs for 70-kDa heat-shock proteins (Hsp70) from two fish species of the genus oryzias. Jpn J Genet 70:423-433. https://doi.org/10.1266/jjg.70.423
  2. Bausero MA, Gastpar R, Multhoff G, Asea A (2005) Alternative mechanism by which IFN-${\gamma}$ enhances tumor recognition: Active release of heat shock protein 72. J Immunol 175:2900-2912. https://doi.org/10.4049/jimmunol.175.5.2900
  3. Bruns AF, Yuldasheva N, Latham AM, Bao L, Pellet-Many C, Frankel P (2012) A heat-shock protein axis regulates VEGFR2 proteolysis, blood vessel development and repair. PLoS ONE 7:e48539. https://doi.org/10.1371/journal.pone.0048539
  4. Currie S, Moyes CD, Tufts BL (2000) The effects of heat shock and acclimation temperature on heat shock protein 70 and heat shock protein30 mRNA expression in rainbow trout: in vivo and in vitro comparisons. J Fish Biol 56:398-408. https://doi.org/10.1111/j.1095-8649.2000.tb02114.x
  5. Currie S, Tufts BL (1997) Synthesis of stress protein 70 (Hsp70) in rainbow trout (Oncorhynchus mykiss) red blood cells. J Exp Biol 200:607-614.
  6. Feder ME, Hofmann GE (1999) Heat-shock proteins, molecular chaperones, and the stress response: Evolutionary and ecological physiology. Annual Reviews Physiology 61:243-282. https://doi.org/10.1146/annurev.physiol.61.1.243
  7. Gething MJ, Sambrook J (1992) Protein folding in the cell. Nature 355:33-45. https://doi.org/10.1038/355033a0
  8. Hunt C, Morimoto RI (1985) Conserved features of eukaryotic HSP70 genes revealed by comparison with the nucleotide sequence of human HSP70. Proc Natl Acad Sci USA 82:6455-6459. https://doi.org/10.1073/pnas.82.19.6455
  9. Im JS, Ghil SH (2013) Cloning of heat shock protein 70 and its expression profile under an increase of water temperature in Rhynchocypris kumgangensis. J Korean Society on Water Environment 29:232-238.
  10. Kim JL, Kong HJ, Kim HS, Kim WJ, Kim DG, Nam BH, Kim YO, An CM (2014) Fish myogenic regulatory protein LUC71: Characterization and expression analysis in Korean rose bitterling (Rhodeus uyekii). Dev Reprod 18:251-258. https://doi.org/10.12717/DR.2014.18.4.251
  11. Kong HJ, Kang HS, Kim HD (1996) Expression of the heat shock proteins in HeLa and fish CHSE-214 cell exposed to heat shcok. Korean J Zool 39:123-131.
  12. Kothary PK, Burgess EA, Candido EPM (1984) The heatshock phenomenon in cultured cells of rainbow trout: hsp70 mRNA synthesis and turnover. Biochim Biophys Acta 783:137-143. https://doi.org/10.1016/0167-4781(84)90005-8
  13. Lele Z, Engel S, Krone PH (1997) Hsp47 and Hsp70 gene expression is differentially regulated in a stress- and tissue-specific manner in zebra-fish embryos. Dev Genet 21:123-133. https://doi.org/10.1002/(SICI)1520-6408(1997)21:2<123::AID-DVG2>3.0.CO;2-9
  14. Lim EH, Brenner S (1999) Short-range linkage relationships, genomic organization and sequence comparisons of a cluster of five hsp70 genes in Fugu rubipes. Cell Mol Life Sic 55:668-678. https://doi.org/10.1007/s000180050323
  15. Lindquist S (1992) Heat-shock proteins and stress tolerance in microorganisms. Curr Opin Genet Dev 2:748-755. https://doi.org/10.1016/S0959-437X(05)80135-2
  16. Lund SG, Caissie D, Cunjak RA, Vijayan MM, Tufts BL (2002) The effects of environmental heat stress on heat shock mRNA and protein expression in Miramachi Atlantic Salmon (Salmo salar) parr. Can J Fish Aquat Sci 59:1553-1562. https://doi.org/10.1139/f02-117
  17. Margulis BA, Antropova OY, Kharazova AD (1989) 70 kDa heat shock proteins from mollusc and human cells have common structural and functional domains. Comp Biochem Physiol 94b:621-623.
  18. Molina A, Biemar F, Muller F, Iyengar A, Prunet P, Maclean N, Martial JA, Muller M (2000) Cloning and expression analysis of an inducible HSP70 gene from tilapia fish. FEBS Letters 474:5-10. https://doi.org/10.1016/S0014-5793(00)01538-6
  19. Moseley P (2000) Stress proteins and the immune response. Immunopharmacology 48:299-302. https://doi.org/10.1016/S0162-3109(00)00227-7
  20. Ojima N, Yamashita M, Watabe S (2005a) Comparative expression anlysis of two paralogous Hsp70s in rainbow trout cells exposed to heat stress. Biochem Biophys Res Commun 1681:99-106.
  21. Ojima N, Yamashita M, Watabe S (2005b) Quantitative mRNA expression profiling of heat-shock protein families in rainbow trout cells. Biochem Biophys Res Commun 329:51-57. https://doi.org/10.1016/j.bbrc.2005.01.097
  22. Pockley AG (2003) Heat shock proteins as regulators of the immune response. Lancet 362:469-476. https://doi.org/10.1016/S0140-6736(03)14075-5
  23. Polanowska Grabowska R, Simon Jr Carl G, Falchetto R, Shabanowitz J, Hunt Donald F, Gear Adrian RL (1997) Platelet adhesion to collagen under flow causes dissociation of a phosphoprotein complex of heat-shock proteins and protein phosphatase 1. Blood 90:1516-1526.
  24. Roberts RJ, Agius C, Saliba C, Bossier P, Sung YY (2010) Heat chock proteins (chaperones) in fish and shellfish and their potential role in relation to fish health: a review. J Fish Dis 33:789-801. https://doi.org/10.1111/j.1365-2761.2010.01183.x
  25. Sanders BM, Martin LS (1993) Stress proteins as biomarkers of contaminant exposure in archived environmental samples. The Science of the Total Environment 139:459-470.
  26. Srivastava P (2002) Roles of heat-shock proteins in innate and adaptive immunity. Nat Rev Immunol 2:185-194. https://doi.org/10.1038/nri749
  27. Welch WJ (1991) The role of heat shock proteins as molecular chaperones. Curr Opin Cell Biol 3:1033-1038. https://doi.org/10.1016/0955-0674(91)90125-I
  28. Wu X, Tan J, Cai M, Liu X (2014) Molecular cloning, characterization, and expression analysis of a heat shock protein (HSP) 70 gene from Paphia undulata. Gene 543:275-285. https://doi.org/10.1016/j.gene.2013.11.103