Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Effects of Feeder Cell Types on Culture of Mouse Embryonic Stem Cell In Vitro

  • Park, Yun-Gwi (Stem Cell Research Center, Jeju National University) ;
  • Lee, Seung-Eun (Stem Cell Research Center, Jeju National University) ;
  • Kim, Eun-Young (Stem Cell Research Center, Jeju National University) ;
  • Hyun, Hyuk (Stem Cell Research Center, Jeju National University) ;
  • Shin, Min-Young (Stem Cell Research Center, Jeju National University) ;
  • Son, Yeo-Jin (Stem Cell Research Center, Jeju National University) ;
  • Kim, Su-Young (Dept. of Preventive Medicine, College of Medicine, Jeju National University) ;
  • Park, Se-Pill (Stem Cell Research Center, Jeju National University)
  • Received : 2015.05.01
  • Accepted : 2015.05.22
  • Published : 2015.09.30
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Abstract

The suitable feeder cell layer is important for culture of embryonic stem (ES) cells. In this study, we investigated the effect of two kinds of the feeder cell, MEF cells and STO cells, layer to mouse ES (mES) cell culture for maintenance of stemness. We compare the colony formations, alkaline phosphatase (AP) activities, expression of pluripotency marker genes and proteins of D3 cell colonies cultured on MEF feeder cell layer (D3/MEF) or STO cell layers (D3/STO) compared to feeder free condition (D3/-) as a control group. Although there were no differences to colony formations and AP activities, interestingly, the transcripts level of pluripotency marker genes, Pou5f1 and Nanog were highly expressed in D3/MEF (79 and 93) than D3/STO (61and 77) or D3/- (65 and 81). Also, pluripotency marker proteins, NANOG and SOX-2, were more synthesized in D3/MEF ($72.8{\pm}7.69$ and $81.2{\pm}3.56$) than D3/STO ($32.0{\pm}4.30$ and $56.0{\pm}4.90$) or D3/- ($55.0{\pm}4.64$ and $62.0{\pm}6.20$). These results suggest that MEF feeder cell layer is more suitable to mES cell culture.

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References

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