Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Quality Characteristics and Evaluation of Physiological Activities of Moju Made with Hovenia dulcis Thunb.

헛개나무를 이용한 모주의 품질 특성 및 생리활성(in vivo) 효능 검증

  • Park, Yeon-Hee (Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Yu, Ok-Kyeong (Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Bae, Cho-Rong (Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Cha, Youn-Soo (Department of Food Science and Human Nutrition, Chonbuk National University)
  • 박연희 (전북대학교 식품영양학과) ;
  • 유옥경 (전북대학교 식품영양학과) ;
  • 배초롱 (전북대학교 식품영양학과) ;
  • 차연수 (전북대학교 식품영양학과)
  • Received : 2015.07.01
  • Accepted : 2015.10.08
  • Published : 2015.11.30
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Abstract

The purpose of this study was to assess the quality characteristics of Moju made with Hovenia dulcis Thunb. and its physiological effects on ICR mice. According to the sensory score, we selected Moju made with 1% Hovenia dulcis Thunb. among Moju made with 0, 0.5, 1, 5, and 10% Hovenia dulcis Thunb. Compared to Moju made without Hovenia dulcis Thunb., Moju made with 1% Hovenia dulcis Thunb. had higher proportions of moisture (86.77 g/100 g) and carbohydrates (11.86 g/100 g). The mean values of the physicochemical analyses were as follows: pH 4.91, acidity 0.28, $^{\circ}Brix$ 12.63, reducing sugar 68.97, alcohol content 0.1, alcohol density 0.998. Moju made with 1% Hovenia dulcis Thunb. did not have effects on DPPH radical scavenging activity; however, superoxide dismutase activity was significantly higher than that of Moju made without Hovenia dulcis Thunb. For assessing physiological activities, 4-week-old male ICR mice were divided into six groups (n=10): normal control group (NC), ethanol-administered group (EC), EC plus low-dose Moju made with 0% Hovenia dulcis Thunb. (MCL), EC plus high-dose Moju made with 0% Hovenia dulcis Thunb. (MCH), EC plus low-dose Moju made with 1% Hovenia dulcis Thunb. (MDL), and EC plus high-dose Moju made with 1% Hovenia dulcis Thunb. (MDH). Serum triglyceride (TG) level was reduced by 11.17% and 19.61% in the MDL and MDH groups, respectively, compared to the EC group. Serum total-cholesterol levels of MDL and MDH groups were significantly lower as compared to the EC group. Serum high-density lipoprotein-cholesterol levels of the MDL and MDH groups were significantly higher than those of the EC group. Liver TG levels were significantly reduced in the MCL and MDL groups. From these results, Moju made with Hovenia dulcis Thunb. demonstrated antioxidant activity and reduction of hyperlipidemia markers. Therefore, Moju made with Hovenia dulcis Thunb. can serve as a non-alcoholic beverage and functional food source.

본 연구는 헛개나무(Hovenia dulcis Thunb.) 함량을 달리한 모주를 제조한 후 관능검사를 통해 최종 레시피를 선정하였으며, 개발 모주를 실험군, 일반 모주를 대조군으로 하여 일반성분, 이화학적 특성 및 항산화 활성을 측정하였다. 생리활성 검증을 위한 동물실험은 6개의 군[증류수섭취군(NC), 알코올섭취군(EC), 알코올+일반 모주 저농도섭취군(MCL), 알코올+일반 모주 고농도섭취군(MCH), 알코올+헛개나무 모주 저농도섭취군(MDL), 알코올+헛개나무 모주 고농도섭취군(MDH)]으로 나눠 실시하였다. 헛개나무 모주의 일반성분 분석 결과 수분 86.77 g/100 g, 탄수화물 11.86 g/100 g을 함유한 것으로 측정되었다. 이화학적 분석결과 수율 60%, pH 4.91, 산도 0.28%, 당도 $12.63^{\circ}Brix$, 환원당 68.97 mg/mL, 알코올 농도 0.1%, 알코올 밀도 $0.998g/cm^3$로 측정되었으며 대조군과 유의적인 차이는 없었다. SOD 활성은 헛개나무 모주가 대조군에 비해 유의적으로 높게 측정되었다. 헛개나무 모주를 실험동물(ICR mice)에게 4주 동안 투여한 결과 혈중 중성지방은 알코올섭취군(EC)에 비해 헛개나무 모주 저농도섭취군(MDL), 헛개나무 모주 고농도섭취군(MDH)에서 낮은 경향을 보였고, 혈중 총 콜레스테롤은 알코올섭취군(EC)에 비해 헛개나무 모주 저농도섭취군(MDL), 헛개나무 모주 고농도섭취군(MDH)에서 유의적으로 낮았다. HDL-콜레스테롤은 알코올섭취군(EC)에 비해 헛개나무 모주 저농도섭취군(MDL), 헛개나무 모주 고농도섭취군(MDH)에서 유의적으로 높았다. 간 중 중성지방은 에탄올섭취군(EC)과 비교하여 일반 모주 저농도섭취군(MCL)과 헛개나무 모주 저농도섭취군(MDL)에서 유의적으로 낮았다. 이상의 결과로 본 연구는 헛개나무 모주를 개발하고, 개발된 모주의 일반성분, 이화학적 특성, 항산화 활성 및 체내 지질 수준을 확인하였으며, 이를 통해 헛개나무를 이용한 모주가 무알코올 음료 및 건강기능성 식품 소재로써 활용 가능한 것으로 사료된다.

Keywords

References

  1. Chang KS, Yu YJ. 1981. Studies on the components of sogukju and commercial yakju. Korean J Food Sci Technol 13: 307-313.
  2. Cha JY, Cho YS. 2001. Biofunctional activities of citrus flavonoids. J Korean Soc Agric Chem Biotechnol 44: 122-128
  3. Jo JH. 1999. The recovery of our traditional liquor. Seohaemunjib, Seoul, Korea. p 168.
  4. Lee BY, Kim SJ, Doo HS, Kwon TH, Kim JW. 2011. Physicochemical and sensory characteristics of Moju sold at restaurants located in Jeonju. Korean J Food Preserv 18: 907-915 https://doi.org/10.11002/kjfp.2011.18.6.907
  5. Kwon YH, Jo SJ, Kim HR, Lee HJ, Kim JH, Ahn BH. 2009. Physicochemical properties and volatile compounds in Jeonju Moju. Korean J Food Sci Technol 41: 503-508.
  6. Choi JY, Kim JH, Kim G, Kim CK, Choi MS. 2014. Effect of fermented Hovenia dulcis Thunb fruit water extract on biomarker for liver injury and body weight changes in rats given oral administration of ethanol. Korean J Food Preserv 21: 412-420. https://doi.org/10.11002/kjfp.2014.21.3.412
  7. Kim MH, Chung YT, Lee JH, Park YS, Shin MK, Kim HS, Kim DH, Lee HY. 2000. Hepatic detoxification activity and reduction of serum alcohol concentration of Hovenia dulcis THUNB from Korea and China. Korean J Medicinal Crop Sci 8: 225-233.
  8. An SW, Kim YG, Kim MH, Lee BI, Lee SH, Kwon HI, Hwang B, Lee HY. 1999. Comparison of hepatic detoxification activity and reducing serum alcohol concentration of Hovenia dulcis THUNB and Alnus japonica Steud. Korean J Medicinal Crop Sci 7: 263-268.
  9. Lee SE, Bang JK, Seong NS. 2004. Inhibitory activity on angiotensin converting enzyme and antioxidant activity of Hovenia dulcis Thunb. cortex extract. Korean J Medicinal Crop Sci 12: 79-84.
  10. Park SH, Chang EY, Chang JS, Yoon KY. 2009. Protective effect of Hovenia dulcis Thumb leaves extract on hepatic injury induced by benzo(${\alpha}$)pyrene in mice. J Korean Soc Food Sci Nutr 38: 569-573. https://doi.org/10.3746/jkfn.2009.38.5.569
  11. Cho JY, Moon JH, Park KH. 2000. Isolation and identification of 3-methoxy-4-hydroxybenzoic acid and 3-methoxy- 4-hydroxycinnamic acid from hot water extracts of Hovenia dulcis Thunb and confirmation of their antioxidative and antimicrobial activity. Korean J Food Sci Technol 32: 1403-1408.
  12. Choi S, Park GS. 2005. A study on the noodle quality made from Hovenia dulcis composite flour. J Korean Soc Food Sci Nutr 34: 1586-1592. https://doi.org/10.3746/jkfn.2005.34.10.1586
  13. Kim HH, Park GS, Jeon JR. 2007. Quality characteristics and storage properties of Sikhe prepared with extracts from Hovenia dulcis THUNB. Korean J Food Cookery Sci 23: 848-857.
  14. Hwang SJ. 2011. Quality characteristics of soybean Dasik containing different amount of Hovenia dulcis Thunb. extract. J East Asian Soc Dietary Life 21: 520-525.
  15. Won SB, Oh KH, Jung SY, Song HS. 2012. Sensory evaluation of Hutgae (Hovenia dulcis Thunb) extract for soy sauce development. Korean J Food & Nutr 25: 266-273. https://doi.org/10.9799/ksfan.2012.25.2.266
  16. Lee JK, Jo HJ, Kim KI, Yoon JA, Chung KH, Song BC, An JH. 2013. Physicochemical characteristics and biological activities of makgeolli supplemented with the fruit of Akebia quinata during fermentation. Korean J Food Sci Technol 45: 619-627. https://doi.org/10.9721/KJFST.2013.45.5.619
  17. Jeon MH, Lee WJ. 2011. Characteristics of blueberry added makgeolli. J Korean Soc Food Sci Nutr 40: 444-449. https://doi.org/10.3746/jkfn.2011.40.3.444
  18. Choi KW, Lee JK, Jo HJ, Lee KJ, Yoon JA, An JH, Chung KH. 2013. Fermentation characteristics of makgeolli made with loquat fruits (Eriobotrya japonica Lindley). J Korean Soc Food Sci Nutr 42: 975-982. https://doi.org/10.3746/jkfn.2013.42.6.975
  19. Lee JS, Lee JS. 2007. Physiological function and development of beverage from Grifola frondosa. J Korean Soc Food Sci Nutr 36: 1241-1247. https://doi.org/10.3746/jkfn.2007.36.10.1241
  20. Park GS, Kim HH. 2005. Physicochemical and sensory characteristics of extract from leaf, fruit stalk and stem of Hovenia dulcis Thunb. J East Asian Soc Dietary Life 15: 65-70.
  21. AOAC. 1995. Official methods of analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC, USA. Chapter 45, p 70.
  22. Kato A, Minoshima Y, Yamamoto J, Adachi I, Watson AA, Nash RJ. 2008. Protective effects of dietary chamomile tea on diabetic complications. J Agric Food Chem 56: 8206- 8211. https://doi.org/10.1021/jf8014365
  23. Tung YT, Wu JH, Hsieh CY, Chen PS, Chang ST. 2009. Free radical-scavenging phytochemicals of hot water extracts of Acacia confusa leaves detected by an on-line screening method. Food Chem 115: 1019-1024. https://doi.org/10.1016/j.foodchem.2009.01.026
  24. Lanoix D, Lacasse AA, Reiter RJ, Vaillancourt C. 2013. Melatonin: the watchdog of villous trophoblast homeostasis against hypoxia/reoxygenation-induced oxidative stress and apoptosis. Mol Cell Endocrinol 381: 35-45. https://doi.org/10.1016/j.mce.2013.07.010
  25. Moon YJ, Cha YS. 2008. Effects of persimmon-vinegar on lipid metabolism and alcohol clearance in chronic alcoholfed rats. J Med Food 11: 38-45. https://doi.org/10.1089/jmf.2007.071
  26. Folch J, Lees M, Sloane Stanley GH. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497-509.
  27. Park CS, Lee TS. 2002. Quality characteristic of takju prepared by wheat flour nuruks. Korean J Food Sci Technol 34: 296-302.
  28. Lee JW, Shim JY. 2010. Quality characteristics of makgeolli during freezing storage. Food Eng Prog 14: 328-334.
  29. Song JC, Park HJ, Shin WC. 2006. Suppression of solid matters precipitation of Takju and its quality improvement by carrageenan. Korean J Food & Nutr 19: 288-295.
  30. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  31. Park YM, Kim SJ, Jo KH, Yang EJ, Jung ST. 2006. Anticariogenic and antioxidant activities from medicinal herbs. J Korean Soc Food Sci Nutr 35: 284-293. https://doi.org/10.3746/jkfn.2006.35.3.284
  32. Joo SY. 2013. Antioxidant activities of medicinal plat extracts. J Korean Soc Food Sci Nutr 42: 512-519. https://doi.org/10.3746/jkfn.2013.42.4.512
  33. Masaki H, Sakaki S, Atsumi T, Sakurai H. 1995. Active-oxygen scavenging activity of plant extracts. Biol Pharm Bull 18: 162-166. https://doi.org/10.1248/bpb.18.162
  34. Baraona E, Lieber CS. 1972. Effects of chronic ethanol feeding on serum lipoprotein metabolism in the rat. J Clin Invest 49: 769-778.
  35. Lee JS, Kim NY, Lee KH, Kim GS, Park HJ, Choi JW, Kim SH. 2000. Effects of flower of Pueraria lobata on lipid peroxidation and activities of alcohol metabolic enzymes in alcohol-treated rats. J Korean Soc Food Sci Nutr 29: 935-942
  36. Rifkind BM, Tamir I, Heiss G, Wallace RB, Tyroler HA. 1979. Distribution of high density and other lipoproteins in selected LRC prevalence study populations: a brief survey. Lipids 14: 105-112. https://doi.org/10.1007/BF02533578
  37. Hong YL, Kim MH, Ahn C, Lee HY, Kim JD. 2000. Studies on the biological activities of the extracts from Hovenia dulcis Thumb. J Agr Sci 11: 1-11.
  38. Decarli LM, Lieber CS. 1967. Fatty liver in the rat after prolonged intake of ethanol with a nutritionally adequate new liquid diet. J Nutr 91: 331-336. https://doi.org/10.1093/jn/91.3_Suppl.331
  39. Lieber CS, Jones DP, DeCarli LM. 1965. Effects of prolonged ethanol intake: production of fatty liver despite adequate diets. J Clin Invest 44: 1009-1021. https://doi.org/10.1172/JCI105200