Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
권호 표지
DOI QR코드

DOI QR Code

Inter simple sequence repeat (ISSR)-PCR based polymorphism of Agaricus bisporus strains and monokayon isolates

Inter simple sequence repeat (ISSR)-PCR에 의한 양송이버섯(Agaricus bisporus) 계통과 단핵균주의 다형성 분석

  • Min, Kyong-Jin (Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Kong, Won-Sik (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kang, Hee-Wan (Graduate School of Future Convergence Technology, Hankyong National University)
  • 민경진 (한경대학교 미래융합기술대학원) ;
  • 공원식 (농촌진흥청 국립특작과학원 버섯과) ;
  • 강희완 (한경대학교 미래융합기술대학원)
  • Received : 2015.08.04
  • Accepted : 2015.09.30
  • Published : 2015.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Twenty Inter simple sequence repeat (ISSR) primers were used to assess genetic diversity of 64 Agaricus strains including 45 A. bisporus strains and other 19 Agaricus spp. ISSR primers, (GA)T, (AG)YC, (GA)C and (CTC) amplified PCR polymorphic bands between the Agaricus species or within A. bisporus strains. PCR polymorphic bands were inputted for UPGMA cluster analysis. The varieties, Saea, Saedo, Saejeong and Saeyeon that have recently been developed in Korea were involved in the same group with closely genetic relationship of coefficient similarity over 0.92, whereas, other Korean strains were genetically related to A. bisporus strains that were introduced from USA, Eroupe and Chinese. Furthermore, ISSR-PCR polymorphism could potentially be used to identify homokaryon isolates.

본 연구에서는 국내외에서 수집한 A. bisporus 45계통과 19 Agaricus spp.를 포함한 64 Agaricus 계통으로부터 genomic DNA를 추출하고 7 종류의 ISSR primer를 사용하여 PCR 다형성 분석을 실시 한 바 (GA)T, (AG)YC, (GA)C and (CTC)의 ISSR primer에서 양송이 계통간 PCR다형성이 관찰 되었다. ISSR-PCR다형성 밴드가 유전적 유사도 산출에 이용되어 UPGMA cluster분석을 적용 dendrogram을 작성 한 결과 A. bisporus의 계통은 7 group으로 분류 되었으며 유사성 함수가 group 간에는 유사성 함수가 0.78에서 0.89의 유연관계를 보였다. 국내에서 최근에 개발된 새정, 새아, 새도, 새연과 교배모본인 ASI1346이 같은 그룹내에서 0.90이상의 유사성함수로 근연관계를 나타내었다. ISSR-PCR다형성 검출 결과 ASI 1038와 유래 단핵균주 S1038297와 ASI 1346S유래 S 737-110는 PCR다형성 밴드가 현저히 적게 증폭 된 것을 확인 할 수 있었다.

Keywords

References

  1. Foulongne-Oriol M. 2009. Novel microsatellite markers suitable for genetic studies in the white button mushroom Agaricus bisporus. Appl Microbiol Biotechnol. 84: 1125-1135. https://doi.org/10.1007/s00253-009-2030-8
  2. Kang HW. 2012. Genetic Divesity Analysis of Fungal Species by Universal Rice Primer (URP)-PCR. Kor J Mycol. 40: 78-85. (in Korea) https://doi.org/10.4489/KJM.2012.40.2.78
  3. Kang HW, Park DS, Park YJ, You CH, Lee BM, Go SJ. 2001. Genomic differentiation among oyster mushroom cultivars released in Korea by URP-PCR fingerprinting. Mycobiology. 29: 85-89.
  4. Khush RS, Becker E, Wach M. 1992. DNA amplification polymor-phisms of the cultivated mushroom Agaricus bisporus. Appl Environ Microbiol. 58: 2971-2977
  5. Mahmudul IN, Fan XL, Bian YB. 2010. Screening of homokaryotic protoclones of Agaricus bisporus(J. Lge) imbach by colony characters and ISSR markers. Bangladesh J Bot. 39: 119-122
  6. Mahmudul IN, Bian YB. 2011. Differentiation of homokaryons and heterokaryons of Agaricus bisporus with inter-simple sequence repeat markers. Microbiol Res. 166:226-236. https://doi.org/10.1016/j.micres.2010.03.001
  7. Malekzadeh K, Shahri BJM, Mohsenifard E. 2011. Use of ISSR markers for strain identification in the button mushroom, Agaricus bisporus. Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products, Arcachon, France pp. 30-34.
  8. May B, Royse DJ. 1982. Confirmation of crosses between lines of Agaricus brunnescens by isozyme analysis. Experimental Mycol. 6: 283-292. https://doi.org/10.1016/0147-5975(82)90121-9
  9. Min KJ, Kim JK, Oh YH, Kong WS, Kang HW. 2014. Genetic Diversity of Agaricus bisporus Strains by PCR Polymorphism. Kor J Mycol. 42: 1-8 (In Korea) https://doi.org/10.4489/KJM.2014.42.1.1
  10. Oh YL, Jang KY, Jhune CS, Kong WS, Yoo YB, Shin PG, Seo JS. 2013. Quality changes in Agaricus bisporus varieties due to period and temperature during their storage. J Mushroom Sci Prod. 11:137-144. (In Korea) https://doi.org/10.14480/JM.2013.11.3.137
  11. Sonnenberg ASM, Johan JPB, Hendrickx PM, Lavrijssen B, Gao W, Weijn A, Mes JJ. 2011. Breeding and strain protection in the button mushroom Agaricus bisporus. Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products, Arcachon, France. pp.7-15.
  12. Wang ZS, Chen MY, Cai ZX, Liao JH, Li HR, Guo ZJ, Lu ZH. 2011. DNA fingerprinting of genetic diversity of Agaricus bisporus. Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products, Arcachon, France, pp. 1-8
  13. Yadav MC, Challen MP, Singh SK, Elliott TJ. 2007. DNA analysis reveals genomic homogeneity and single nucleotide polymorphism in 5.8S ribosomal RNA gene spacer region among commercial cultivars of the button mushroom Agaricus bisporus in India. Curr Sci. 93:1383-1391.