Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Inhibitory Effects of Ssangbohwan on Osteoclast Differentiation and Bone Resorption

쌍보환 추출물의 파골세포 분화 억제와 골 흡수 억제효과

  • Kim, Seong Joung (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Jeong Ju (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kim, June Hyun (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Jo, So Hyun (Department of Oriental Medical Ophthalmology & Otolaryngology & Dermatology, College of Oriental Medicine, Wonkwang University) ;
  • Park, Min Cheol (Department of Oriental Medical Ophthalmology & Otolaryngology & Dermatology, College of Oriental Medicine, Wonkwang University) ;
  • Jo, Eun Heui (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Wonkwang University)
  • 김성중 (원광대학교 한의과대학 침구의학교실) ;
  • 이정주 (원광대학교 한의과대학 침구의학교실) ;
  • 김준현 (원광대학교 한의과대학 침구의학교실) ;
  • 조소현 (원광대학교 한의과대학 안이비인후피부학교실) ;
  • 박민철 (원광대학교 한의과대학 안이비인후피부학교실) ;
  • 조은희 (원광대학교 한의과대학 침구의학교실)
  • Received : 2015.08.06
  • Accepted : 2015.09.09
  • Published : 2015.09.20
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Abstract

Purpose : The first purpose of this study is to find out whether the water extract of Rehmanniae Radix Preparat(RRP), Cuscutae Semen(CS) and their combination(Ssangbohwan, SBH) have the effect of suppressing Receptor activator of nuclear factor kappa-B ligand(RANKL)-induced osteoclast differentiation. The second purpose of this study is to find out whether the water extract of RRP, CS and SBH have the effect of inhibiting osteoporosis in an osteoporosis model induced by lipopolysaccharide(LPS). Methods : After promoting differentiation of osteoclasts by treating the RANKL, we observed the effect by the administration of RRP, CS and SBH. In addition, by means of Reverse transcription polymerase chain reaction(RT-PCR), we assayed mRNA expression levels of NFATc1, c-Fos, TRAP and GAPDHS(Glyceraldehyde-3-phosphate dehydrogenase, spermatogeni) from bone marrow macrophages(BMMs). Similarly, the protein expression levels of NFATc1 (Nuclear factor of activated T-cells, cytoplasmic1), C-Fos, MAPKs(Mitogen-activated protein kinases) and ${\beta}$-actin in cell lysates were analyzed by means of Western Blotting. Finally, we determined the anti-osteoporotic effects of RRP, CS and SBH, through the use of Lipopolysaccharide-induced bone-loss mouse. Results : RRP, CS and SBH showed remarkable inhibitive effect on RANKL-treated osteoclast differentiation without cytotoxicity. SBH inhibited the phosphorylation of p38, Jun N-terminal kinases(JNK), and I-${\kappa}B$ and down-regulated the induction of c-Fos and NFATc1 by RANKL. RRP, CS suppressed degradation of I-${\kappa}B$, but it did not affect c-Fos and NFATc1 by RANKL. Lastly, in vivo data showed that RRP and SBH prevented bone erosion by LPS treatment. Conclusions : These results demonstrate SBH can be effective remedy for bone-loss diseases such as osteoporosis.

Keywords

References

  1. Ministry of Health & Welfare. 2008 national health statistics [Internet]. Sejong City [cited 2010 March 18]. table2-62. Available from : http://www.mw.go.kr/front_new/jb/sjb030301vw.jsp?PAR_MENU_ID=03&MENU_ID=0321&CONT_SEQ=264493
  2. Goldmann DR. American college of physicians Complete hone medical guide. New York : DK Publishing, Inc. 2003 : 368.
  3. Statistical Information Center. Health insurance review & assessment service [Internet]. Seoul [cited 2013 January 31] Available from : http://www.hira.or.kr/dummy.do?pgmid=HIRAA020041000000&cmsurl=/cms/notice/02/1316013_24959.html
  4. Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature. 2003 ; 423(6937) : 337-42. https://doi.org/10.1038/nature01658
  5. Kim JY. Effects of Cuscuta japonica Chois on gene expression in RANKL-induced RAW 264.7 cell. Spkom. 2010 ; 14(2) : 77-89.
  6. Kim JK, Kim SW, Lee BE, Hwang HH, Kwon JS, Ko SY. The effect of herbal formulation(OPB) rehmannia gutinosa lbosch and eleutherococcus senticosus max extracts on bone density and bone biochemical marker in ovariectomized rats. J Oral Med Pain. 2007 ; 32(1) : 35-43.
  7. Park JW, Choi SS, Kim CW, Park BR. Effects of Ssangbohwan on the ovariectomized rat model of postmenopausal osteoporosis. The Journal of Traditional Korean Medicine. 2000 ; 10(1) : 241-54.
  8. Cho SI. Effects of the Rehmanniae Radix Preparat on ovariectomized rats. Kor J Herbology. 2005 ; 20(4) : 61-7.
  9. Kim JH, Kim HS, Jun JY et al. Effects of Cuscutae Semen extract on prevention of osteoporosis in ovariectomized rats. J Korean Obstet Gynecol. 2012 ; 25(4) : 1-11. https://doi.org/10.15204/JKOBGY.2012.25.4.001
  10. Shin CS, Choi Hj, Kim MJ et al. Prevalence and risk factors of osteoporosis in Korea : a communitybased cohort study with lumbar spine and bone mineral density. Bone. 2010 ; 47 : 378-87. https://doi.org/10.1016/j.bone.2010.03.017
  11. Katzung BG. Basic & clinical pharmacology. Seoul : Daehan Medical Books. 2002 : 771-89.
  12. Chung YS. The medical treatment of osteoporosis. Korean J Med. 2010 ; 79(3) : 250-3.
  13. Ann JY, Kim JH, Ki JY, Kwak HB, Oh JM, Kim YK. Inhibitory effects of water extract of cervi parvum cornu, carthami tinctorii fructus and combination on osteoclast differentiation and bone resorption. HFS. 2010 ; 19(2) : 167-82.
  14. Lee JW, Seo BI, Park JH, Roh SS, Kim YH, Kim MR. Effect of mantidis ootheca and mori fructus on treatment of osteoporosis in ovariectomized rats. Kor J Herbology. 2009 ; 24(1): 59-71.
  15. Hwang JS, Seo BI, Park JH et al. Effect of cervi cornu on treatment of osteoporosis in ovariectomized rats. Kor J Herbology. 2010 ; 25(2) : 1-10.
  16. Kim JH. Review of recent research on the osteoporosis - is mainly dependent on the oriental medicine clinical and experimental study. The Acupuncture. 1998 ; 15(2) : 437-54.
  17. Kim WH, Choi DY. Jangboobyeonjeungnonchi. Seoul : Sungbosa. 1988 : 286, 293.
  18. Ann JY, Ki JY, Kim JH et al. A review of antiosteoporosis effects of herbal extracts in Korean journals. Herbal Formula Science. 2008 ; 16(2) : 47-69.
  19. Jin J. Suchinyangnosinseo.(Heumjeongsagojeonseojaboo) Seoul : Daesungmoonhwasa. 1995 : 386.
  20. Xu HG. Commonly used Chinese medicine knowledge. Shanghai : Shanghai Science and Technology Press. 1983 : 174-5.
  21. Sin MG. Clinical herbal medicine. Seoul : Younglimsa 2006 : 226-7, 248-51.
  22. Sun SM. Qian Jin Yi Fang. Taibei : Free Press. 1982 : 19.
  23. Lee CH. Uihak-ipmun. Seoul : Hansungsa. 1984 : 194-5.
  24. Beijing University of Chinese Medicine Teaching with WH Group with Prescription Medicine. Four subway vernacular solutions include medicinal song. Beijing : People's Health Publishing House. 1978 : 4-5.
  25. Zhongshan Medical College. Clinical application of traditional Chinese medicine. Guangdong : Guangdong People's Publishing House. 1975 : 82-4, 374-6.
  26. Lee GU, Woo HW, Park YS. Modern clinical psychiatry. Seoul : Hana-Uihaksa. 1988 : 495-501.
  27. Kim DH. Pharmacology of traditional oriental medicine. Seoul : Shinil Books. 2005 : 690-1.
  28. Jinfeng Z. Experimental and clinical studies on the effect of Shou Tai Wan and additives on threatened abortion. Journal of Integrated Traditional and Western Medicine. 1987 ; 7 : 407.
  29. Linmao Y. Integrating Chinese and western medicine to treat infantile uterus. Herbal Journal of Traditional Chinese Medicine. 1992 ; 14(4) : 40.
  30. Xiumei D. Researches on semen cuscutae botanicals. Research and Development of Chinese Medicine in Chang Bai Shan. 1992 ; 1(2) : 53.
  31. Matsuo K, Galson DL, Zhao C et al. Nuclear factor of activated T-cells(NFAT) rescues osteoclastogenesis in precursors lacking c-Fos. J Biol Chem. 2004 ; 279(25) : 26475-80. https://doi.org/10.1074/jbc.M313973200
  32. Dougall WC, Glaccum M, Charrier K et al. RANK is essential for osteoclast and lymph node development. Genes Dev. 1999 ; 13(18) : 2412-24. https://doi.org/10.1101/gad.13.18.2412
  33. Takayanagi H. Mechanistic insight into osteoclast differentiation in osteoimmunology. J Mol Med. 2005 ; 83(3) : 170-9. https://doi.org/10.1007/s00109-004-0612-6
  34. Takayanagi H, Kim S, Taniguchi T. Signaling crosstalk between RANKL and interferons in osteoclast differentiation. Arthritis Res. 2002 ; 4(3) : 227-32. https://doi.org/10.1186/ar581
  35. Shimizu H, Nakagami H, Tsukamoto I et al. NFkappaB decoyoligodeoxynucleotides ameliorates osteoporosis through inhibition of activation and differentiation of osteoclasts. Gene Ther. 2006 ; 13(12) : 933-41. https://doi.org/10.1038/sj.gt.3302711
  36. Qureshi ST, Larivire L, Leveque G et al. Endotoxin-tolerant mice have mutations in toll-like receptor 4(Tlr4). J Exp Med. 1999 ; 189(4) : 615-25. https://doi.org/10.1084/jem.189.4.615
  37. Abu-Amer Y, Ross FP, Edwards J, Teitelbaum SL. Lipopolysaccharide-stimulated osteoclastogenesis is mediated by tumor necrosis factor via its P55 receptor. J Clin Invest. 1997 ; 100(6) : 1557-65. https://doi.org/10.1172/JCI119679
  38. Lee JA, Ban HR, Yang SJ, Cho SH, Jin CS. Effects of the Rehmanniae Radix Preparat on osteoporotic tats induced by ovariectomy. J Korean Obstet Gynecol. 2004 ; 17(4) : 112-24.
  39. Kim JH, Kim HS, Jun JY et al. Effects of Cuscutae Semen extract on prevention of osteoporosis in ovariectomized rats. J Korean Obstet Gynecol. 2012 ; 25(4) : 1-11. https://doi.org/10.15204/JKOBGY.2012.25.4.001