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Quality characteristics of fermented vinegar prepared with the detoxified Rhus verniciflua extract

무독화 옻 추출물로 제조한 발효식초의 품질 특성

  • Baek, Seong Yeol (Department of Agro-food Resources, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Choong Hwan (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Park, Yoo Kyoung (Department of Medical Nutrition, Kyunghee University) ;
  • Choi, Han-Seok (Department of Agro-food Resources, National Institute of Agricultural Sciences, RDA) ;
  • Mun, Ji-Young (Department of Agro-food Resources, National Institute of Agricultural Sciences, RDA) ;
  • Yeo, Soo-Hwan (Department of Agro-food Resources, National Institute of Agricultural Sciences, RDA)
  • 백성열 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 이충환 (건국대학교 생명공학과) ;
  • 박유경 (경희대학교 의학영양학과) ;
  • 최한석 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 문지영 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 여수환 (농촌진흥청 국립농업과학원 농식품자원부)
  • Received : 2015.07.15
  • Accepted : 2015.08.21
  • Published : 2015.10.30

Abstract

In this study, vinegar was produced using urushiol-free fermented Rhus verniciflua extract to create a lacquer with added value. The effect of manufacturing conditions on the quality of vinegar using detoxified R. verniciflua extract for fermentation was investigated. The acidity of the vinegar for inoculations with various liquid starter contents was 4.8~4.9%, and it was similar among all treatment groups. The acidity of vinegar was higher when the initial alcohol content was high. The acetic acid yields were 82.8%, 84.4%, 77.7%, and 69.5%, and the maximum yield was observed when the initial alcohol content was 6%. For acetic acid fermentation using different amounts of detoxified R. verniciflua extracts, the acidity of the vinegar with the extract after fermentation was 5.3~5.9%. However, the acidity of vinegar without the extract was 5.5%. The intensity of the brown color was high for vinegar without the extract. Hunter's L values were high for vinegar with an extract content of 2%. Acetic acid (53.3~65.8 mg/mL) was the predominant acid. Arginine ($190.3{\sim}333.3{\mu}g/mL$), proline ($125.6{\sim}290.8{\mu}g/mL$), alanine ($126.1{\sim}270.9{\mu}g/mL$), and glutamic acid ($159.0{\sim}262.4{\mu}g/mL$) were the predominant amino acids in detoxified R. verniciflua vinegar.

본 연구에서는 옻의 활용과 부가가치 증대를 위해 우루시올 성분이 제거된 무독화 옻 추출물을 첨가한 식초제조 조건 및 품질 특성을 조사하였다. 종초 접종량을 달리하여 무독화 옻 추출물 첨가한 식초의 초산 발효에서는 발효 후반 종초 접종량에 따른 산도는 4.8~4.9%로 차이가 나타나지 않았다. 초기 알코올 농도에 따른 초산 발효에서는 초기 알코올 농도가 높을수록 산도가 높은 경향을 나타내었으며, 초산 수율은 82.8%, 84.4%, 77.7% 및 69.5%로, 초기 알코올 농도 6% 시험구에서 가장 높았으며, 알코올 8% 시험구에서 낮은 것으로 나타났다. 무독화 옻 추출물 농도에 따른 초산 발효에서는 옻 추출물 첨가한 식초의 산도는 5.3~5.9%로 무첨가 식초의 산도 5.5%에 비해 0.4% 높았으며, 옻 추출물 농도에 따른 차이는 크지 않았다. 무독화 옻 추출물 농도에 따른 갈색도는 무첨가 식초가 옻 추출물 첨가 식초보다 갈색의 정도가 높았으며 색도는 옻 추출물 2% 첨가 식초의 명도가 가장 높게 나타났다. 무독화 옻 추출물 농도에 따른 주요 유기산은 acetic acid로 분석되었으며 함량은 53.3~65.8 mg/mL의 범위를 나타내었다. 무독화 옻식초의 주요 아미노산은 arginine($190.3{\sim}333.3{\mu}g/mL$), proline($125.6{\sim}290.8{\mu}g/mL$), alanine($126.1{\sim}270.9{\mu}g/mL$), glutamic acid($159.0{\sim}262.4{\mu}g/mL$)이며, 무첨가 식초에 비해 높은 유리 아미노산은 alanine과 glutamic acid로 나타났다.

Keywords

References

  1. Kwon SH, Jeong EJ, Lee GD, Jeong YJ (2000) Reparation method of fruit vinegars by two stage fermentation and beverages including vinegar. Food Ind Nutr, 5, 18-24
  2. Kim DH (1999) Studies on the production of vinegar from fig. J Korean Soc Food Sci Nutr, 28, 53-60
  3. Lee WJ, Kim SS (1998) Preparation of Sikhe with brown rice. Korean J Food Sci Technol, 30, 146-150
  4. Jeong YJ, Lee MH (2000) A view and prospect of vinegar using Kyungpook special products (persimmon, apple and grape). Food Ind Nutr, 5, 53-59
  5. MFDS (2014) Korean Food Standards Codex. Ministry of Food & Drug Safety, Cheongju, Korea, p 56, 165-166
  6. Jung NC (1998) Biological activity of urushiol and flavonoids from Lac tree (Rhus verniciflua Stokes). Ph D Thesis. Chonnam National University, Gwangju, Korea, p 9-21
  7. Kim JB (2003) Identification of antioxidative component from stem bark of Rhus verniciflua. Korean J Food Nutr, 16, 60-65
  8. Lee JC, Kim J, Lim KT, Jang YS (2002) Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and anti-apoptotic properties. Biochim Biophys Acta, 1570, 181-191 https://doi.org/10.1016/S0304-4165(02)00196-4
  9. Son YO, Lee KY, Lee JC, Jang HS, Kim JG, Jeon YM, Jang YS (2005) Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines. Toxicol Lett, 135, 115-125
  10. Park KY, Jung GO, Lee KT, Choi JW, Choi MY, Kim GT, Jung JJ, Park HJ (2004) Antimutagenic activity of flavonoids from the heartwood of Rhus verniciflua. J Ethnopharmacol, 90, 73-79 https://doi.org/10.1016/j.jep.2003.09.043
  11. Lee JD, Huh JE, Jeon GS, Yang HR, Woo HS, Choi DY, Park DS (2009) Flavonol-rich RVHxR from Rhus verniciflua Stokes and its major compound fisetin inhibits inflammation-related cytokines and angiogenic factor in rheumatoid arthritic fibroblast-like synovial cells and in vivo models. Int Immunopharmacol, 9, 268-276 https://doi.org/10.1016/j.intimp.2008.11.005
  12. Lee DS, Jeong GS, Li B, Park H, Kim YC (2010) Anti-inflammatory effects of sulfuretin from Rhus verniciflua Stokes via the induction of heme oxygenase-1 expression in murine macrophages. Int Immunopharmacol, 10, 850-858 https://doi.org/10.1016/j.intimp.2010.04.019
  13. Kim JS, Kwon YS, Chun WJ, Kim TY, Sun J, Yu CY, Kim MJ (2010) Rhus verniciflua Stokes flavonoid extracts have anti-oxidant, antimicrobial and ${\alpha}$-glucosidase inhibitory effect. Food Chem, 120, 539-543 https://doi.org/10.1016/j.foodchem.2009.10.051
  14. KFS (2008) The investigation of forestry management of Korea. Korea Forest Service, Daejeon, Korea, p 12-19
  15. Choi HS, Yeo SH, Jeong ST, Choi JH, Park HS, Kim MK (2012) Preparation and characterization of urushiol free fermented Rhus verniciflua stem bark (FRVSB) extracts. Korean J Food Sci Technol, 44, 173-178 https://doi.org/10.9721/KJFST.2012.44.2.173
  16. Choi HS, Yeo SH, Jeong ST, Choi JH, Kang JE, Kim MK (2012) Effect of the extracts from fermented-Rhus verniciflua stem bark with fomitella fraxinea on the growth and enzyme activity of soybean productfermenting microorganisms. Korean J Mycol, 40, 235-243 https://doi.org/10.4489/KJM.2012.40.4.235
  17. Choi HS, Kim MK, Park HS, Yun SE, Mun SP, Kim JS, Sapkota K, Kim S, Kim TY, Kim SJ (2007) Biological detoxification of lacquer tree (Rhus verniciflua Stoles) stem bark by mushroom species. Food Sci Biotechnol, 16, 935-942
  18. Baek SY, Park HY, Lee CH, Yeo SH (2014) Comparison of the fermented property and isolation of acetic-acid bacteria from traditional Korean vinegar. Korean J Food Preserv, 21, 903-907 https://doi.org/10.11002/kjfp.2014.21.6.903
  19. Chen XH, Lou WY, Zong MH, Smith TJ (2011) Optimization of culture conditions to produce high yields of active Acetobacter sp. CCTCC M209061 cells for anti-Prelog reduction of prochiral ketones. BMC Biotech, 11, 110-121 https://doi.org/10.1186/1472-6750-11-110
  20. NAAS (2013) Quality analysis manual for fermented food. Semyeongmunhwasa, National Academy of Agricultural Science, RDA, Wanju, Korea, p 93-94
  21. Hitachi High-Technologies Corporation. L-8900 Amino Acid Analyzer. (2014) Available from: http://www.hitachihitec.com/global/science/lc/l8900.html#jump2. Accessed
  22. Kim YT, Seo KI, Jung YJ, Lee YS, Shin KH (1997) The production of vinegar using citron (Citrus junos Seib) juice. J East Soc Dietary Life, 7, 301-307
  23. Jeong YJ, Seo JH, Park NY, Shin SR, Kim GS (1999) Changes in the components of persimmon vinegars by two stages fermentation. Korean J Postharvest Sci Technol, 6, 228-232
  24. Seo JH, Jeong YJ, Kim JN, Woo CJ, Yoon SR, Kim DH (2001) Quality comparison of potato vinegars produced by various Acetobacter bacteria. Korean J Postharvest Sci Technol, 8, 60-65
  25. Kim MH, Choi UK (2006) Acetic acid fermentation by Acetobacter sp. SK-7 using Maesil juice. Korean J Food Culture, 21, 420-425
  26. Hong SM, Kang MJ, Lee JH, Jeong JH, Kwon SH, Seo KI (2012) Production of vinegar using Rubus coreanus and its antioxident activities. Korean J Food Preserv, 19, 594-603 https://doi.org/10.11002/kjfp.2012.19.4.594
  27. Kim YD, Kang SH, Kang SG (1996) Studies on the acetic acid fermentation using maesil juice. J Korean Soc Food Sci Nutr, 25, 695-700
  28. Oh YJ (1992) A study on cultural conditions for acetic acid production employing pear juice. J Korean Soc Food Nutr, 21, 377-380
  29. Kim KE, Choi OS, Lee YJ, Kim HS, Bae TJ (2001) Processing of vinegar using the sea tangle (Laminaria japonica) extract. Korean J Life Sci, 11, 211-217
  30. Woo SM, Yeo SH, Kwon JH, Kim SH, Jeong YJ (2014) Quality characteristics of high-acidity vinegar prepared with grape juice. Korean J Food Preserv, 22, 100-107
  31. Lee SW, Kwon JH, Yoon SR, Woo SM, Yeo SH, Jeong YJ (2011) Quality characteristics of brown rice vinegar prepared sing varying amounts of nuruk (an amylolytic enzyme preparation) and employing different fermentation conditions. Korean J Food Preserv, 18, 26-32 https://doi.org/10.11002/kjfp.2011.18.1.026
  32. Joo KH, Cho MH, Park KJ, Jeong SW, Lim JH (2009) Effects of fermentation method and brown rice content on quality characteristics of brown rice vinegar. Korean J Food Preserv, 16, 33-39
  33. Nakanc S (1988) Food useful for preventing alcohol in toxication containing persimmon-vinegar and optimum fruits, with blood alcohol concentration reducing action. Japan Patent 63, 562-566
  34. Woo SM, Jo YJ, Lee SW, Kwon JH, Yeo SH, Jeong YJ (2012) Quality comparison of static-culture and commercial brown rice vinegars. Korean J Food Preserv, 19, 301-307 https://doi.org/10.11002/kjfp.2012.19.2.301
  35. Woo SM, Yeo SH, Kwon JH, Kim SH, Jeong YJ (2015) Quality characteristics of high-acidity vinegar prepared grape juice. Korean J Food Preserv, 22, 100-107 https://doi.org/10.11002/kjfp.2015.22.1.100
  36. Shin JS, Jeong YJ (2003) Changes in the components of acetic acid fermentation of brown rice using raw starch digesting enzyme. J Korean Soc Food Sci Nutr, 32, 381-387 https://doi.org/10.3746/jkfn.2003.32.3.381

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