Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Development of a Single-nucleotide Polymorphism Marker for the Sw-5b Gene Conferring Disease Resistance to Tomato spotted wilt virus in Tomato

  • Lee, Hyung Jin (Department of Horticultural Biotechnology and Institute of Life Science & Resources, College of Life Science, Kyung Hee University) ;
  • Kim, Boyoung (Department of Horticultural Biotechnology and Institute of Life Science & Resources, College of Life Science, Kyung Hee University) ;
  • Bae, Chungyun (Department of Horticultural Biotechnology and Institute of Life Science & Resources, College of Life Science, Kyung Hee University) ;
  • Kang, Won-Hee (Department of Plant Science, Seoul National University) ;
  • Kang, Byoung-Cheorl (Department of Plant Science, Seoul National University) ;
  • Yeam, Inhwa (Horticulture and Breeding Major, School of Bioresource Sciences, Andong National University) ;
  • Oh, Chang-Sik (Department of Horticultural Biotechnology and Institute of Life Science & Resources, College of Life Science, Kyung Hee University)
  • Received : 2015.02.24
  • Accepted : 2015.06.12
  • Published : 2015.10.31
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Abstract

Tomato spotted wilt virus (TSWV) causes one of the most destructive viral diseases that threatens global tomato production. Sw-5b was reported as the resistance gene effective against TSWV. The objective of this research was to develop a single-nucleotide polymorphism (SNP) marker to distinguish tomato cultivars resistant to TSWV from susceptible cultivars for marker-assisted breeding. First, we determined genotypes for TSWV resistance in 32 commercial tomato cultivars using the previously reported Sw-5b gene-based marker. Then, DNA sequences of Sw-5b alleles in tomato cultivars showing resistant or susceptible genotypes were analyzed; a single SNP was found to distinguish tomato cultivars resistant to TSWV from susceptible cultivars. Based on the confirmed SNP, a SNP primer pair was designed. Using this new SNP sequence and high-resolution melting analysis, the same 32 tomato cultivars were screened. The results were perfectly correlated with those from screening with the Sw-5b gene-based marker. These results indicate that the SNP maker developed in this study will be useful for better tracking of resistance to TSWV in tomato breeding.

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References

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  1. Natural Resources Resistance to Tomato Spotted Wilt Virus (TSWV) in Tomato (Solanum lycopersicum) vol.22, pp.20, 2015, https://doi.org/10.3390/ijms222010978