The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Antioxidant and Antimicrobial Properties of Various Solvent Extracts from Robus idaeus

추출 용매에 따른 산딸기 추출물의 항산화 및 항균 활성

  • Lee, Mi-Hee (Dept. of Food and Nutrition and Cook, Taegu Science University) ;
  • Lee, Jeong-Eun (Dept. of Food and Nutrition and Cook, Taegu Science University)
  • 이미희 (대구과학대학교 식품영양조리과) ;
  • 이정은 (대구과학대학교 식품영양조리과)
  • Received : 2015.07.24
  • Accepted : 2015.09.23
  • Published : 2015.10.31
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Abstract

The objective of the present study was to determine the total phenol and flavonoid contents and to evaluate the antioxidant potential, of different solvent extracts (ethyl acetate, n-butanol, chloroform, and water) from Robus idaeus in various radical scavenging models (DPPH activity, superoxide dismutase (SOD) activity, reducing power, and nitrite scavenging activity), along with their antimicrobial potential. Measurement of total phenol and flavonoid content of the ethyl acetate extract of R. idaeus was found to be significantly higher than those of the other extracts. The ethyl acetate extract (at a concentration of $1,000{\mu}g/mL$) showed significantly higher reducing power and DPPH radical scavenging activity as compared to the other extracts. Results were dose-dependent. Moreover, the ethyl acetate extract of R. idaeus ($1,000{\mu}g/mL$) showed potent antioxidant efficacy ($85.5{\pm}1.18%$) as evidenced by nitrite scavenging ability at pH 1.2. All solvent extracts of R. idaeus showed lower SOD-like activity (13.72~20.54%). In addition, the antimicrobial activity of all solvent extracts except water extract showed strong inhibition (inhibitory zones in mm) of Staphylococcus aureus ($19.40{\pm}1.00mm$) and Bacillus cereus ($20.50{\pm}0.21mm$) growth. In particular, ethyl acetate extracts (100 mg/mL) showed antimicrobial activity comparable to that of tetracycline (0.01 mg/mL), which was used as a positive control. The results of this study indicate that the ethyl acetate extract of R. idaeus is a natural antioxidant and antimicrobial, with enriched phenols and flavonoids concentration, that has potential in the development of health-enhancing food products.

Keywords

References

  1. Ancos B, Gonzalez EM, Cano MP. 2000. Ellagic acid, vitamin C, and total phenolic contents and radical scavenging capacity affected by freezing and frozen storage in raspberry. J Agric Food Chem 48:4565-4570 https://doi.org/10.1021/jf0001684
  2. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181:1198-1200
  3. Choi JW, Yoo YM, Kim MY, Nam JH, Nugroho A, Park HJ. 2008. Anti-hyperlipidemic and anti-hyperlipidemic effects of the triterpenoid-rich fractions from Rubus coreanus and Rubus crataegifolius and their main component, niga-ichigoside F1, in Streptozotocin-induced diabetic rats. Natural Product Sciences 14:260-264
  4. Choi SY, Cho HS, Sung NJ. 2006. The antioxidative and nitrite scavenging ability of solvent extracts from wild grape (Vitis coignetiea) skin. J Korean Soc Food Sci Nutr 35:961-966 https://doi.org/10.3746/jkfn.2006.35.8.961
  5. Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12:239-249.
  6. Gordon MF. 1990. The mechanism of antioxidant action in vitro. In B. J. F. Hudson, Food Antioxidants. London: Elsevier Applied Science p 1-18
  7. Han WC, Ji SH, Surh J, Kim MH, Lee JC, Kim SH, Jang KH. 2010. Effect of supplementation of Rubus crataegifolius on fermentation characteristics of Rosa rugosa wine. J East Asian Dietary Life 20:321-327
  8. Hwang CR, Hwang IG, Kim HY, Kang TS, Kim YB, Joo SS, Lee JS, Jeong HS. 2011. Antioxidant compound and activity of dropwort (Oenanthe javanica) ethanol extracts. J Korean Soc Food Sci Nutr 40:316-320 https://doi.org/10.3746/jkfn.2011.40.2.316
  9. Jang KW, Park SH, Ha SD. 2003. Market trends in functional foods. Food Sci Industry 36:17-25
  10. Jeon SM, Lee JY, Kim HW, Lee YM, Jang HH, Hwang KA, Kim HR, Park DS. 2012, Antioxidant activity of extracts and fractions from Aster scaber. J Korean Soc Food Sci Nutr 41:1197-1204 https://doi.org/10.3746/jkfn.2012.41.9.1197
  11. Jeong HJ, Kim SA, Kwon JH, Kim HK. 2008. Physiological activities of Gardeniae fructus extracts by microwave-assisted extraction as affected by solvents. J Korean Soc Food Sci Nutr 37:282-287 https://doi.org/10.3746/jkfn.2008.37.3.282
  12. Ju MJ, Do JR, Kwon JH, Kim HK. 2009. Physiological activities of mistletoe extracts from Viscum album L. J Korean Soc Food Sci Nutr 38:529-534 https://doi.org/10.3746/jkfn.2009.38.5.529
  13. Kang DY, Shin MO, Shon JH, Bae SJ. 2009. The antioxidative and antimicrobial effects of Celastrus orbiculatus. J Life Sci 19:52-57 https://doi.org/10.5352/JLS.2009.19.1.052
  14. Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds (in Korean). Korean Food Sci Technol 28:232-239
  15. Kim DS, Ahn BW, Yeum DM, Lee DH, Kim SB, Park YH. 1987a. Degradation of carcinogenic nitrosamine formation factor by natural food components. 1. Nitrite scavenging effect of vegetable extracts. Bull Korean Fish Soc 20:463-468
  16. Kim DS, Ahn BW, Yeum DM, Lee DH, Park YH, Kim DS. 1987b. Degradation of carcinogenic nitrosamine formation factor by natural food components. 2. Nitrite scavenging effect of seaweed extracts. Bull Korean Fish Soc 20:469-473
  17. Kim JY, Lee JA, Park SY. 2007. Antibacterial activities of Oenothera laciniata extracts. J Korean Soc Food Sci Nutr 36:255-261 https://doi.org/10.3746/jkfn.2007.36.3.255
  18. Kim KH, Kim HJ, Byun MW, Yook HS. 2012. Antioxidant and antimicrobial activities of ethanol extract from six vegetables containing different sulfur compounds. Korean Soc Food Sci Nutr 41:577-583 https://doi.org/10.3746/jkfn.2012.41.5.577
  19. Kim MJ, Byun MW, Jang MS. 1996. Physiological and antibacterial activity of bamboo (Sasa coreana Nakai) leaves. J Korean Soc Food Nutr 25:135-142
  20. Kim SM, Cho YS, Sung SK. 2001. The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sci Technol 33:626-632
  21. Lee HH, Moon YS, Yun HK, Park PJ, Kwak EJ. 2014. Contents of bioactive constituents and antioxidant activities of cultivated and wild raspberries. Kor J Hort Sci Technol 32:115-122
  22. Lee SO, Lee Hj, Yu MH, Im HG, Lee IS. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J Food Sci Technol 37:233-240
  23. Lim JA, Yun BW, Baek SH. 2007. Antioxidative activity and nitrite scavenging ability of methanol extract from Salvia plebeia R. Br. Korean J Medicinal Crop Sci 15:183-188
  24. Nam JH, Jung HJ, Tapondjou LA, Lee KT, Choi JW, Kim WB, Park HJ. 2007. The anti-hyperlipidemic effect and constituents of the $19\alpha$-hydroxyursane-type triterpenoid fraction obtained from the leaves of Rubus crataegifolius. Natural Product Sciences 13:152-159
  25. Oyaizu M. 1986. Studies of products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J Nutr 44:307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  26. Park YO, Lim HS, 2009. Antioxidant activities of bamboo (Sasa borealis) leaf extract according to extraction solvent. J Korean Soc Food Sci Nutr 38:1640-1648 https://doi.org/10.3746/jkfn.2009.38.12.1640
  27. Yu MH, Chae IG, Jung YT, Kim HI, Lee IS. 2011. Antioxidative and antimicrobial activities of methanol extract from Rosmarinus officinalis L. and their fractions. J Life Sci 21:375-384 https://doi.org/10.5352/JLS.2011.21.3.375
  28. Zhang L, Li J, Hogan S, Chung H, Welbaum GE, Zhou K. 2010. Inhibitory effect of raspberries on starch digestive enzyme and their antioxidant properties and phenolic composition. Food Chem 119:592-599 https://doi.org/10.1016/j.foodchem.2009.06.063

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