Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Selection and Characterization of Bacillus licheniformis MH48 for the Biocontrol of Pine Wood Nematode (Bursaphelenchus xylophilus)

소나무재선충 생물학적 방제를 위한 Bacillus licheniformis MH48의 선발 및 특성 규명

  • Jeong, Min-Hae (Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Yang, Seo-Young (Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Lee, Yong-Sung (Division of Applied Bioscience and Biotechnology, Chonnam National University) ;
  • Ahn, Young-Sang (Division of Forest Resources, Chonnam National University) ;
  • Park, Yun-Serk (Purne Co., Ltd., Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Han, Hye-rim (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute) ;
  • Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, Chonnam National University)
  • 정민해 (전남대학교 응용생물공학부) ;
  • 양서영 (전남대학교 응용생물공학부) ;
  • 이용성 (전남대학교 응용생물공학부) ;
  • 안영상 (전남대학교 산림자원학부) ;
  • 박윤석 ((주)푸르네) ;
  • 한혜림 (국립산림과학원 산림병해충연구과) ;
  • 김길용 (전남대학교 응용생물공학부)
  • Received : 2015.03.09
  • Accepted : 2015.06.17
  • Published : 2015.09.30
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Abstract

Pine wilt disease (PWD) caused by pine wood nematode, Bursaphelenchus xylophilus, has become the most serious threat to pine trees in Korea. This study was subjected to investigate effective biological control agent against PWD. To select nematocidal bacteria against PWD, Bacillus licheniformis MH48 was selected among five bacteria due to its high nematocidal potential. B. licheniformis MH48 was tested for cell growth and protease activity to evaluate its nematicidal potential. In the B. licheniformis MH48, cell numbers were highest three days after incubation, while protease activity was highest after seven days. In the effect of different concentrations of B. licheniformis MH48 culture broth against B. xylophilus, 20% concentration of culture broth showed approximately 80% of pine wood nematode mortality compared to the control. Especially, pine wood nematode's cuticle layers were degraded two days after treatment of B. licheniformis MH48 culture broth. The present study suggests that B. licheniformis MH48 can be one of the potential biocontrol candidates against pine wood nematode due to its ability to produce protease.

소나무재선충(Bursaphelenchus xylophilus)에 의해서 발생하는 소나무재선충병은 우리나라 소나무림에 피해를 주고 있다. 본 연구는 친환경 소나무재선충 방제제 개발을 위하여 살선충 활성이 뛰어난 미생물을 선발하고자 실험을 수행하였다. 소나무재선충에 대하여 살선충 효과를 나타내는 미생물을 선발하기 위해 5종 미생물의 배양액 처리를 통해 살선충 활성이 뛰어난 Bacillus licheniformis MH48을 선발하였다. B. licheniformis MH48의 살선충 효과를 검증하기 위해 세포 생육과 단백질 분해 효소 활성도를 분석하였는데, 세포 생육은 배양 3일째 가장 높았고 단백질 분해 효소 활성은 7일째에 가장 높은 활성을 보였다. 또한, B. licheniformis MH48의 배양액 농도에 따른 소나무재선충의 살선충 활성을 조사한 결과, 키틴-젤라틴(CG) 배지와 키틴-선충(CN) 배지 모두 20% 배양액 처리시 치사율이 80% 이상으로 나타났다. 특히, B. licheniformis MH48 배양액 처리 후 시간이 지남에 따라 소나무재선충의 표피가 분해되는 것을 관찰하였다. 이러한 결과로 B. licheniformis MH48은 소나무재선충을 생물학적으로 방제할 수 있는 방제제로서 가능성과 가치가 있다고 사료된다.

Keywords

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