Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
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Effect of Olive Flounder, Paralichthys olivaceus, Immune Genes Stimulation by Molecular Adjuvant in Vitro Culture Condition

In vitro 조건에 따른 molecular adjuvant의 넙치, Paralichthys olivaceus 면역유전자 자극 효과

  • Received : 2015.09.09
  • Accepted : 2015.10.12
  • Published : 2015.10.31
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Abstract

Adjuvant is an immune enhancer commonly used during vaccination to enhance the host immune response. In the present study, we produced the several recombinant protein from immune related gene of olive flounder (Paralichthys olivaceus). Especially, to produce the soluble type of recombinant protein, we constructed the MBP (Maltose binding protein) fusion G-CSF (Granulocyte colony stimulating factor) recombinant protein among the flounder immune related genes. To verify the immune stimulatory effect and safety of this recombinant protein (rPoGCSF), expression changes of several immune genes were tested using quantitative real-time PCR method with gene specific primer from flounder head kidney leukocytes. As a result, we confirmed that the rPoGCSF has an ability of immune stimulatory effect, also it has broad range of pH and temperature.

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References

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