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Dynamics of the mobile insert helix in the domain III-IV of Aux/IAA17 probed by site-directed spin labeling and paramagnetic NMR spectroscopy

  • Han, Mookyoung (Department of Agricultural Biotechnology, Seoul National University) ;
  • Suh, Jeong-Yong (Department of Agricultural Biotechnology, Seoul National University)
  • 투고 : 2015.08.02
  • 심사 : 2015.09.25
  • 발행 : 2015.10.10

초록

The plant hormone auxin is involved in all stages of plant development. Aux/IAAs are the transcriptional repressors that bind to the Auxin Response Factors (ARFs) to regulate the gene expression upon auxin release. Aux/IAA have highly conserved C-terminal domains (domains III-IV) that mediate both homotypic and heterotypic interactions between Aux/IAA and ARF family proteins. Recent studies revealed that the conserved domains III-IV share a common ${\beta}$-grasp fold that oligomerizes in a front-to-back manner. In particular, Aux/IAA contains a mobile insert helix in the domain III-IV, whereas ARFs do not. Here, we investigated the dynamics of the insert helix using paramagnetic NMR spectroscopy. The insert helix exhibited fast motions in the ps-ns time scale from $^{15}N$ relaxation data, but the amplitude of the motion is likely limited to the local neighborhood. Our result suggests that the motion of the helix may have functional implications in protein-protein interactions for transcriptional regulations.

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참고문헌

  1. M. Han, Y. Park, I. Kim, E. H. Kim, T. K. Yu, S. Rhee, and J. Y. Suh, Proc. Natl. Acad. Sci. USA, 111, 18613 (2014) https://doi.org/10.1073/pnas.1419525112
  2. H. M, Nanao, T. Vinos-Poyo, G. Brunoud, E. Thevenon, M .Mazzoleni, Mast, and R. Dumas, Nat. commun., 5 (2014)
  3. D. A. Korasick, C. S. Westfall, S. G. Lee, Nanao, H. M, R. Dumas, G. Hagen, and L. C. Strader, Proc. Natl. Acad. Sci. USA, 111, 5427 (2014) https://doi.org/10.1073/pnas.1400074111
  4. D. C. Dinesh, M. Kovermann, M. Gopalswamy, A. Hellmuth, L. I. A. C. Villalobos, and H. Lilie, S.Abel, .Proc. Natl. Acad. Sci. USA, 112, 6230(2015) https://doi.org/10.1073/pnas.1424077112
  5. T. Zheng, A. Boyle, H. R. Marsden, D. Valdink, G. Martelli, and J. Raap, A. Kros, Org. Biomol. Chem., 13, 1159 (2015) https://doi.org/10.1039/C4OB02125H
  6. J. Y. Suh, T. K. Yu, Y. J. Yun, and K. O. Lee, J. Kor. Magn. Reson. Soc. 18, 1 (2014)
  7. I. G. Lee, K. Y. Lee, J. H. Kim, S. Chae, and B. J. Lee, J. Kor. Magn. Reson. Soc., 17, 54 (2013) https://doi.org/10.6564/JKMRS.2013.17.1.054

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