Phylogenetic diversity of marine bacteria dependent on the port environment around the Ulleng Island

울릉도 항구의 해양환경에 따른 해양미생물의 분포 변화

  • 강용호 (영남대학교 생명공학부) ;
  • 안민경 (영남대학교 생명공학부)
  • Received : 2015.08.25
  • Accepted : 2015.09.24
  • Published : 2015.09.30


Pyrosequencing of 16S rDNA tags was used to obtain the bacterial diversity and community structure in the uncultured seawaters as well as in the cultured seawaters, which were collected from the 7 ports (Cheonbu, Hyunpo, Taeha, Namyang, Sadong, Dodong, and Jeodong) and 1 seashore (Guam) around the Ulleng island, Korea. Alphaproteobacteria were the most abundant group in the clean seawaters such as seawaters of Taeha and Sadong ports. Gammaproteobacteria proportion increased depending upon the wastewater amounts mixed with the seawaters such as seawaters of Namyang, Dodong, and Jeodong ports. The genuses of Alteromonas (from samples of Cheonbu, Taeha, Guam, Namyang, Sadong), Shewanella (from sample of Jeodong), and Vibrio (from samples of Hyunpo and Dodong) were dominant group in each of the cultured seawaters incubated in marine broth (Difoco). The results suggest that the incoming wastewaters to the port seawaters contribute to the dynamic change of the marine bacterial community around the Ulleng island.

울릉도에서 7곳의 항구(천부항, 현포항, 태하항, 남양항, 사동항, 도동항, 저동항)와 1곳의 해변(구암)에서 표층해수를 채취하였다. 배양하지 않은 시료(uncultured samples)와 배양한 시료(cultured samples)에서 각각 미생물의 16S rDNA를 pyrosequencing하여 해양미생물의 분포를 조사하였다. 태하항과 사동항의 해수처럼 청정한 해수에서는 Alphaproteobacteria 분포율이 높았고, 남양항, 도동항, 저동항의 해수처럼 생활하수나 하천수의 유입이 많은 곳에서는 Gammaproteobacteria 분포율이 증가하였다. Marine broth로 배양한 시료(cultured samples)에서는 Alteromonas (천부항, 태하항, 구암해변, 남양항, 사동항), Shewanella (저동항), Vibrio (현포항, 도동항) 속이 우점으로 분포하였다. 본 연구결과는 항구로 유입되는 하천수나 생활하수가 해양미생물의 분포에 큰 변화를 초래한다는 것을 시사한다.


Supported by : 국토해양부


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