Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지
DOI QR코드

DOI QR Code

Hepatoprotective effect of Bifidobacterium adolescentis SPM0212 on carbon tetrachloride induced hepatotoxicity

사염화탄소로 유도된 간 손상에 대한 비피도박테리움 어돌레센티스 SPM0212의 보호효과

  • Received : 2015.08.03
  • Accepted : 2015.09.22
  • Published : 2015.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Probiotics are microbial food supplements or components of bacteria which have traditionally been added to dairy foods for extra health boost. Our aim was to evaluate the hepatoprotective effect of Bifidobacterium adolescentis SPM0212 as probiotics, which we previously found has potential anti-hepatitis B virus activity. The study was conducted using Wistar albino rats and probiotics were treated orally for 9 days consecutively and acute liver injury was induced by administration of carbon tetrachloride ($CCl_4$) on the 7th and 8th days. Liver damage was assessed by quantifying serum activities of glutamate oxaloacetate transaminase (SGOT) and glutamate pyruvate transaminase (SGPT), as well as by histopathological examination. B. adolescentis SPM0212 significantly prevented the elevation of SGOT and SGPT levels, and reduced the negative effect of $CCl_4$ on body and organ weights. Histopathological study revealed the livers of the carbon tetrachloride treated rats showed almost complete loss of normal hepatocyte architecture, but that rats treated with B. adolescentis SPM0212 showed minimal damage and normal hepatocyte architecture. Our results suggest that B. adolescentis SPM0212 be considered useful probiotics for protecting the liver from xenobiotics and hepatitis B virus, and as well as useful as a functional food for maintaining human health.

프로바이오틱스는 미생물 식품 보조제 또는 건강증진을 위해 전통적으로 유제품에 첨가되어온 세균의 구성요소이다. 본 연구에서는 프로바이오틱스로 이용되는 Bifidobacterium adolescentis SPM0212 균주의 간 보호효과를 평가하였으며, 이전 연구에서는 B형 간염 바이러스에 항 바이러스 활성을 나타내는 균주로 확인되었다. 본 연구는 Wistar albino 랫드를 이용하여 수행되었으며 프로바이오틱스를 연속해서 9일 동안 경구로 투여하고 7일째와 8일째에는 사염화탄소를 투여하여 급성 간 손상을 유도하였다. 간 손상 정도는 혈중 glutamate oxaloacetate transaminase (SGOT)와 glutamate pyruvate transaminase (SGPT)의 수치와 병리조직학적 시험을 통해 평가하였다. 그 결과, B. adolescentis SPM0212는 SGOT와 SGPT 수치의 증가를 유의적으로 억제하였으며, 사염화탄소가 체중과 장기무게에 미치는 부정적인 영향을 감소시켰다. 또한 병리조직학적 시험결과, 사염화탄소만 투여한 랫드의 간은 정상적인 간세포의 구조가 거의 소실된 반면에 사염화탄소와 B. adolescentis SPM0212를 투여한 랫드의 간은 아주 적은 손상과 정상적인 간세포의 구조를 가지고 있었다. 따라서 본 연구결과 B. adolescentis SPM0212 균주는 건강 유지를 위한 기능성 식품뿐만 아니라 제노바이오틱스나 B형 간염 바이러스로 부터 간을 보호하기위한 프로바이오틱스로 유용하게 사용될 수 있음을 시사한다.

Keywords

References

  1. Adawi, D., Kasravi, F.B., Molin, G., and Jeppsson, B. 1997. Effect of Lactobacillus supplementation with and without arginine on liver damage and bacterial translocation in an acute liver injury model in the Rat. Hepatology 25, 642-647. https://doi.org/10.1002/hep.510250325
  2. Han, S.Y., Huh, C.S., Ahn, Y.T., Lim, K.S., Baek, Y.J., and Kim, D.H. 2005a. Hepatoprotective effect of lactic acid bacteria. J. Microbiol. Biotechnol. 15, 887-890.
  3. Han, S.Y., Huh, C.S., Ahn, Y.T., Lim, K.S., Baek, Y.J., and Kim, D.H. 2005b. Hepatoprotective effect of lactic acid bacteria, inhibitors of $\beta$-glucuronidase production against intestinal microflora. Arch. Pharm. Res. 28, 325-329. https://doi.org/10.1007/BF02977800
  4. Jain, M., Kapadia, R., Jadeja, N.R., Thounaojam, C.M., Ranjitsinh, V.D., and Mishra, S.H. 2011. Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf. Asian Pac. J. Trop. Biomed. 10, 443-447.
  5. Kataria, J., Li, N., Wynn, J.L., and Neu, J. 2009. Probiotic microbes: do they need to be alive to be beneficial?. Nutr. Rev. 67, 546-550. https://doi.org/10.1111/j.1753-4887.2009.00226.x
  6. Kim, J.H., Kim, H.J., Son, J.H., Chun, H.N., Yang, J.O., Choi, S.J., Paek, N.S., Choi, G.H., and Kim, S.K. 2003. Effect of Lactobacillus fermentum MG590 on alcohol metabolism and liver function in rats. J. Microbiol. Biotechnol. 13, 919-925.
  7. Kim, Y., Lee, D., Kim, D., Cho, J., Yang, J., and Chung, M. 2008. Inhibition of proliferation in colon cancer cell lines and harmful enzyme activity of colon bacteria by Bifidobacterium adolescentis SPM0212. Arch. Pharm. Res. 31, 468-473. https://doi.org/10.1007/s12272-001-1180-y
  8. Kitada, M., Igarashi, K., Hirose, S., and Kitagawa, H. 1979. Inhibition by polyamines of lipid peroxide formation in rat liver microsomes. Biochem. Biophys. Res. Commun. 87, 388-394. https://doi.org/10.1016/0006-291X(79)91808-4
  9. Kumaravelu, P., Dakshinamoorthy, D.P., Subramaniam, S., Devaraj, H., and Devaraj, N.S. 1995. Effect of eugenol on drugmetabolizing enzymes of carbon tetrachloride-intoxicated rat liver. Biochem. Pharmacol. 49, 1703-1707. https://doi.org/10.1016/0006-2952(95)00083-C
  10. Lee, D.K., Kang, J.Y., Shin, H.S., Park, I.H., and Ha, N.J. 2013. Antiviral activity of Bifidobacterium adolescentis SPM0212 against Hepatitis B virus. Arch. Pharm. Res. 36, 1525-1532. https://doi.org/10.1007/s12272-013-0141-3
  11. Lin, C.C. and Huang, P.C. 2000. Antioxidant and hepatoprotective effects of Acathopanax senticosus. Phytother. Res. 14, 489-494. https://doi.org/10.1002/1099-1573(200011)14:7<489::AID-PTR656>3.0.CO;2-G
  12. Low, D., Thomas, N.W., and Fry, J.R. 1995. Lobar variation of carbon tetrachloride hepatotoxicity in the rat. Toxicol. Lett. 81, 1-4. https://doi.org/10.1016/0378-4274(95)03405-6
  13. Luna, L.G. 1968. Manual histology staining methods of armed forces institute of pathology, pp. 1-31. 3rd ed. McGraw-Hill, New York, USA.
  14. Martin, P. and Fiedman, L.S. 2012. Assessment of liver function and diagnostic studies, pp. 1-19. In Fiedman, L.S. and Keefe, E.B. (eds.), Handbook of liver diseases, 3rd ed. Churchill Livingstone, Philadelphia, USA.
  15. McCormack, S.A. and Johnson, L.R. 1991. Role of polyamines in gastrointestinal mucosal growth. Am. J. Physiol. 260, 795-806.
  16. Muriel, P. 2007. Some experimental models of liver damage, pp. 119-130. In Sahu, S.C. (ed.), Hepatotoxicity: from genomics to in vitro and in vivo models. John Wiley & Sons Ltd., Chichester, UK.
  17. Nallamilli, B.R., Kumar, C.P., Reddy, K.V., Prasanna, M.L., Maruthi, V., and Sucharita, P. 2013. Hepatoprotective activity of Cichorium intybus (Linn.) root extract against carbon tetrachloride induced hepatotoxicity in albino Wistar rats. Drug Invent. Today 5, 311-314. https://doi.org/10.1016/j.dit.2013.08.005
  18. Nanji, A.A., Khettry, U., and Sadrzadeh, S.M.H. 1994. Lactobacillus feeding reduces endotoxemia and severity of experimental alcoholic liver (disease). Proc. Soc. Exp. Bio. Med. 205, 243-247. https://doi.org/10.3181/00379727-205-43703
  19. Nurrochmad, A., Margono, S.A., Sardjiman, Hakim, A.R., Ernawati, Kurniawati, E., and Fatmawati, E. 2013. Hepatoprotective and antioxidant activity of pentagamavunon-0 against carbon tetrachloride-induced hepatic injury in rats. Asian Pac. J. Trop. Biomed. 6, 438-442. https://doi.org/10.1016/S1995-7645(13)60070-X
  20. Osman, N., Adawi, D., Ahrne, S., Jeppsson, B., and Molin, G. 2007. Endotoxin- and D-galactosamine induced liver injury improved by the administration of Lactobacillus, Bifidobacterium and blueberry. Dig. Liver Dis. 39, 849-856. https://doi.org/10.1016/j.dld.2007.06.001
  21. Ou, C., Chiu, Y., Lin, S., Chang, Y., Huang, H., and Lin, M. 2012. Hepatoprotective effect of lactic acid bacteria in the attenuation of oxidative stress from tert-butyl hydroperoxide. J. Food Drug Anal. 20, 101-110.
  22. Parvez, S., Malik, K.A., Ah Kang, S., and Kim, H.Y. 2006. Probiotics and their fermented food products are beneficial for health. J. Appl. Microbiol. 100, 1171-1185. https://doi.org/10.1111/j.1365-2672.2006.02963.x
  23. Plaza-Diaz, J., Gomez-Llorente, C., Abadia-Molina, F., Saez-Lara, M.J., Cpmpana-Martin, L., Munoz-Quezada, S., Romero, F., Gil, A., and Fontana, L. 2014. Effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCm I-4035 and Lactobacillus rhamnosus CNCM I-4036 on hepatic steatosis in Zucker rats. PLoS One 9, e98401. https://doi.org/10.1371/journal.pone.0098401
  24. Recknagel, R.O., Glende Jr., E.A., Dolak, J.A., and Waller, R.L. 1989. Mechanisms of carbon tetrachloride toxicity. Pharmacal. Ther. 43, 139-154. https://doi.org/10.1016/0163-7258(89)90050-8
  25. Reid, G. 2005. The importance of guidelines in the development and application of probiotics. Curr. Pharm. Des. 11, 11-16. https://doi.org/10.2174/1381612053382395
  26. Samal, P.K. and Dangi, J.S. 2014. Isolation, preliminary characterization and hepatoprotective activity of polysaccharides from Tamarindus indica L. Carbohydr. Polym. 102, 1-7. https://doi.org/10.1016/j.carbpol.2013.10.059
  27. Solga, S.F. 2003. Probiotics can treat hepatic encephalopathy. Med. Hypotheses 61, 307-313. https://doi.org/10.1016/S0306-9877(03)00192-0
  28. Teselkin, Y.O., Babenkova, I.V., Kolhir, V.K., Baginskaya, A.I., Tjukavkina, N.A., Kolesnik, Y.A., Selivanova, I.A., and Eichholz, A.A. 2000. Dihydroquercetin as a means of antioxidative defence in rats with tetrachloromethane hepatitis. Phytother. Res. 14, 160-162. https://doi.org/10.1002/(SICI)1099-1573(200005)14:3<160::AID-PTR555>3.0.CO;2-Y
  29. Uemitsu, N. and Nakayoshi, H. 1984. Evaluation of liver weight changes following a single oral administration of carbon tetrachloride in rats. Toxicol. Appl. Pharmacol. 75, 1-7. https://doi.org/10.1016/0041-008X(84)90069-3
  30. Wang, G.F., Shi, L.P., Ren, Y.D., Liu, Q.F., Liu, H.F., Zhang, R.J., Li, Z., Zhu, F.H., He, P.L., Tang, W., et al. 2009. Anti-hepatitis B virus activity of chlorogenic acid, quinic acid and caffeic acid in vivo and in vitro. Antiviral Res. 83, 186-190. https://doi.org/10.1016/j.antiviral.2009.05.002
  31. Wang, Y., Li, Y., Xie, J., Zhang, Y., Wang, J., Sun, X., and Zhang, H. 2013. Protective effects of probiotic Lactobacillus casei Zhang against endotoxin- and D-galactosamine-induced liver injury in rats via anti-oxidative and anti-inflammatory capacities. Int. Immunopharmacol. 15, 30-37. https://doi.org/10.1016/j.intimp.2012.10.026
  32. Xing, H.C., Li, L.J., Xu, K.J., Shen, T., Chen, Y.B., Sheng, J.F., Chen, Y., Fu, S.Z., Chen, C.L., Wang, J.G., et al. 2006. Protective role of supplement with foreign Bifidobacterium and Lactobacillus in experimental hepatic ischemia-reperfusion injury. J. Gastroenterol. Hepatol. 21, 647-656. https://doi.org/10.1111/j.1440-1746.2006.04306.x
  33. Yun, J.H., Kim, Y.A., Chung, M.J., Kang, B.Y., and Ha, N.J. 2007. Hepatoprotective and anti-fatigue effects of lactic acid bacteria (Lactobacillus acidophilus, Bifidobacterium bifidum and Streptococcus thermophilus). J. Toxicol. Pub. Health 23, 11-17.
  34. Zedan, Z.K. 2011. Hepatoprotective effect of Lactobacillus plantarum against Salmonella typhimurium in mice. J. Al-Nahrain Univ. 14, 126-131. https://doi.org/10.22401/JNUS.14.4.17
  35. Zhao, H.Y., Wang, H.J., Lu, Z., and Xu, S.Z. 2004. Intestinal microflora in patients with liver cirrhosis. Chin. J. Dig. Dis. 5, 64-67. https://doi.org/10.1111/j.1443-9573.2004.00157.x